Hydroxocobalamin acetate
Based on 3 publication(s) in Google Scholar
Hydroxocobalamin acetate is an injectable natural vitamin B12. Hydroxocobalamin acetate binds to NO. Hydroxocobalamin acetate detoxifies Cyanide and NaSH. Hydroxocobalamin acetate reduces hypotension. Hydroxocobalamin acetate is used in research related to vitamin B12 deficiency, including pernicious anemia.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.90%
- CAS. Nr.: 22465-48-1
- Formel: C64H91CoN13O16P
- Molecular Weight:1388.39
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Speicherung:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Chemical Information
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Publications Citing Use of MedChemExpress (MCE) Hydroxocobalamin acetate
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Biologische Aktivität
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Human Endogenous Metabolite |
Hydroxocobalamin (1 mM) acetate shows no significant improvement in key mitochondrial respiratory states and no attenuation of superoxide production in Cyanide-poisoned peripheral blood mononuclear cells (PBMC) and human aortic smooth muscle cells (HASMC)[4].
Hydroxocobalamin (50-100 μM) acetate prevents hepatocyte cytotoxicity induced by NaSH in isolated rat hepatocytes[8].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Hydroxocobalamin (25-50 mg/kg; i.v.; 14 days) acetate significantly reduces Cyanide toxicity in rats ingesting Amygdalin (HY-N0190)[6].
Hydroxocobalamin (75-150 mg/kg; i.v.) acetate significantly reduces mortality from Potassium cyanide poisoning in adult beagle dogs, and is associated with cardiovascular recovery and improvement in acid-base status[7].
Hydroxocobalamin (0.25 mmol/kg; i.p.; 2 min after NaSH administration) acetate effectively prevents NaSH-induced death in mice at 24 h[8].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar strain albino rats (weight 150-165 g) with Cyanide poisoning induced by oral Amygdalin ingestion[6]
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Dosage:25 mg/kg or 50 mg/kg
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Administration:Intravenous injection, 14 days
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Result:Reduced blood cyanide and serum lactate levels in a dose-dependent manner.
Normalized packed cell volume (PCV), haemoglobin concentration, and blood pH.
Prevented histological alterations in the liver, with no significant histological change in the colour and size of the hepatic lobes.
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Animal Model:Adult beagle dogs (male and female, mean weight 8.9-9.2 kg) with acute cyanide poisoning induced by intravenous Potassium cyanide infusion[7]
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Dosage:75 mg/kg or 150 mg/kg
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Administration:Intravenous infusion
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Result:Significantly reduced mortality rate.
Caused 15 of 19 animals to survive through 14 days at 75 mg/kg, and all 18 animals survived through 14 days at 150 mg/kg.
Led to rapid cardiovascular recovery, with increased blood pressure starting 1-3 min after infusion.
Improved acid-base status, with lower arterial lactate concentrations and higher arterial pH values.
Chemical Information
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CAS. Nr. 22465-48-1
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Appearance Solid
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Molecular Weight 1388.39
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Formel C64H91CoN13O16P
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Color Brown to black
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SMILES
C[C@@]12[N]([Co+3]34([N]5=C[N@@]([C@@H](O[C@H](CO)[C@H]6OP7([O-])=O)[C@@H]6O)C8=C5C=C(C)C(C)=C8)([O-]C(C)=O)[N-]9C2[C@H](CC(N)=O)[C@@](C)(CCC(NC[C@H](C)O7)=O)C9=C(C)C([C@@H](CCC(N)=O)C%10(C)C)=[N]3C%10=CC%11=[N]4C([C@](CC(N)=O)(C)[C@@H]%11CCC(N)=O)=C%12C)=C%12[C@@H](CCC(N)=O)[C@]1(C)CC(N)=O
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Synonyms
Vitamin B12a acetate
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
2025 Jan 31;16(1):1233. PMID: 39890804 -
Nat Commun
Design of modular autoproteolytic gene switches responsive to anti-coronavirus drug candidates. [Abstract]2021 Nov 22;12(1):6786. PMID: 34811361 -
J Clin Transl Hepatol
Hydrogen Sulfide Promotes Platelet Autophagy via PDGFR-α/PI3K/Akt Signaling in Cirrhotic Thrombocytopenia. [Abstract]2024 Jul 28;12(7):625-633. PMID: 38993511
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (72.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (1.80 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (300 KB)
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SDS (419 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Cheungpasitporn W, et al. High-dose hydroxocobalamin for vasoplegic syndrome causing false blood leak alarm. Clin Kidney J. 2017 Jun;10(3):357-362. [Content Brief]
[2]. Wang H, et al. Oral vitamin B12 versus intramuscular vitamin B12 for vitamin B12 deficiency. Cochrane Database Syst Rev. 2018 Mar 15;3:CD004655. [Content Brief]
[3]. Greenberg SS, et al. Hydroxocobalamin (vitamin B12a) prevents and reverses endotoxin-induced hypotension and mortality in rodents: role of nitric oxide. J Pharmacol Exp Ther. 1995 Apr;273(1):257-65. [Content Brief]
[4]. Owiredu S, et al. In vitro comparison of hydroxocobalamin (B12a) and the mitochondrial directed therapy by a succinate prodrug in a cellular model of cyanide poisoning. Toxicol Rep. 2020 Sep 17;7:1263-1271. [Content Brief]
[5]. Greenberg SS, et al. Hydroxocobalamin (vitamin B12a) prevents and reverses endotoxin-induced hypotension and mortality in rodents: role of nitric oxide. J Pharmacol Exp Ther. 1995 Apr;273(1):257-65. [Content Brief]
[7]. Borron SW, et al. Efficacy of hydroxocobalamin for the treatment of acute cyanide poisoning in adult beagle dogs. Clin Toxicol (Phila). 2006;44 Suppl 1:5-15. [Content Brief]
[8]. Truong DH, et al. Prevention of hydrogen sulfide (H2S)-induced mouse lethality and cytotoxicity by hydroxocobalamin (vitamin B(12a)). Toxicology. 2007 Dec 5;242(1-3):16-22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7203 mL | 3.6013 mL | 7.2026 mL | 18.0065 mL |
| 5 mM | 0.1441 mL | 0.7203 mL | 1.4405 mL | 3.6013 mL | |
| 10 mM | 0.0720 mL | 0.3601 mL | 0.7203 mL | 1.8006 mL | |
| 15 mM | 0.0480 mL | 0.2401 mL | 0.4802 mL | 1.2004 mL | |
| 20 mM | 0.0360 mL | 0.1801 mL | 0.3601 mL | 0.9003 mL | |
| 25 mM | 0.0288 mL | 0.1441 mL | 0.2881 mL | 0.7203 mL | |
| 30 mM | 0.0240 mL | 0.1200 mL | 0.2401 mL | 0.6002 mL | |
| 40 mM | 0.0180 mL | 0.0900 mL | 0.1801 mL | 0.4502 mL | |
| 50 mM | 0.0144 mL | 0.0720 mL | 0.1441 mL | 0.3601 mL | |
| 60 mM | 0.0120 mL | 0.0600 mL | 0.1200 mL | 0.3001 mL |
Hydroxocobalamin acetate Related Classifications
HY-B2209B Related Classifications
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