Anti-Mouse TREM2 Antibody (178)
Based on 1 Customer Validation
Anti-Mouse TREM2 Antibody (178) is an antibody targeting mouse TREM2, which blocks the binding of ligands to TREM2 and inhibits TREM2-mediated signaling pathways. Anti-Mouse TREM2 Antibody (178) inhibits the tumor growth of MCA/1956 sarcoma in mice. Anti-Mouse TREM2 Antibody (178) enhances IFNγ production by intratumoral CD8+ T cells and TNFα production by intratumoral CD4+ T cells. Anti-Mouse TREM2 Antibody (178) can be used in the research of sarcoma, colorectal cancer and breast tumors. The recommended isotype control is Rat IgG2a kappa, Isotype Control (HY-P990679).
For research use only. We do not sell to patients.
- Purity: 98.92%
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Rat IgG2a kappa
Mouse
|
TREM-2 |
TNF-α |
The Anti-Mouse TREM2 Antibody (178) (20 μg/mL; overnight) blocks ligand-induced activation of mouse TREM2 in stably transfected 2B4 reporter cells at a concentration of 20 μg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:C57BL/6J mice (mixed sexes, 8 weeks old at tumor injection, subcutaneous injection of 1×106 MCA/1956 sarcoma cells)[1]
-
Dosage:200 μg/mouse
-
Administration:i.p.; every 5 days; starting day 2 after tumor injection
-
Result:Significantly reduced MCA/1956 tumor growth compared to control antibody treatment.
Induced complete tumor regression in all treated mice when combined with suboptimal anti-PD-1.
Reduced the frequency of Ly6C+MHCII- and CD64+ myeloid subsets at day 10 after tumor injection when combined with suboptimal anti-PD-1.
Reduced the representation of Ly6CᵐᵒʷMHCII-, CD206+, CD63+, and CD9+ macrophage subsets at day 24, with no change in total macrophage numbers.
Augmented IFNγ production by intratumoral CD8+ T cells and TNFα production by intratumoral CD4+ T cells via ex vivo stimulation.
Was more effective at reducing tumor growth than constitutive TREM2 deficiency.
Had no off-target effects when administered to Trem2-/- mice.
Q99NH8
TREM-2
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
Flow cytometry; ELISA; in vitro TREM-2 blockade; in vivo TREM-2 blockade
Chemical Information
-
Appearance Liquid
-
Color Colorless to light yellow
-
SMILES
[Anti-Mouse TREM2 Antibody (178)]
-
Formulation
Please refer to the lot-specific COA for specific buffer information.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
-
Data Sheet (269 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse TREM2 Antibody (178) Related Classifications
HY-P991754 Related Classifications
Powered by Bioz