Estradiol benzoate
Based on 17 publication(s) in Google Scholar
Estradiol benzoate (β-Estradiol 3-benzoate) is a HBx protein inhibitor and inhibits androgen and hepatitis B virus (HBV) transcription, replication. Estradiol benzoate shows antifertility effects, anti- Toxoplasma gondii activity and can improve memory behavior of Ovariectomy (Ovx) female mice.
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- Pureté: 99.90%
- CAS No.: 50-50-0
- Formule: C25H28O3
- Masse moléculaire:376.49
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Solvant et solubilité
- Pureté et documentation
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Publications Citing Use of MedChemExpress (MCE) Estradiol benzoate
More- J Nanobiotechnology. 2023 Mar 8;21(1):81. [Abstract]
- Redox Biol. 2026 May:92:104101. [Abstract]
- Phytomedicine. 2024 Nov 14:136:156227. [Abstract]
- J Med Chem. 2024 Sep 12;67(17):15387-15410. [Abstract]
- Anal Chem. 2026 Jun 16;98(23):16962-16970. [Abstract]
- J Ethnopharmacol. 2019 Dec 5:245:112181. [Abstract]
- Bioorg Chem. 2025 Feb 1:156:108235. [Abstract]
- Molecules. 2022 Aug 6;27(15):5000. [Abstract]
- Front Cell Dev Biol. 2022 Jul 22:10:854425. [Abstract]
- iScience. 2023 Oct 7;26(11):108158. [Abstract]
- Reprod Biomed Online. 2023 Nov;47(5):103304. [Abstract]
- Mol Med Rep. 2019 Mar;19(3):2144-2152. [Abstract]
- J Pharmacol Sci. 2024 Apr;154(4):236-245. [Abstract]
- Am J Transl Res. 2022 Apr 15;14(4):2184-2198. [Abstract]
- Trop J Pharm Res. 2019; 18 (11): 2349.
- SSRN. 2023 Jun 19.
- Evid Based Complement Alternat Med. 2022 Aug 30;2022:1406820. [Abstract]
Customer Validation & Images
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Histological Imaging/Staining
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
Activité biologique
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Bax |
Estradiol benzoate (5-75 μM, 24 h-48 h) has a downregulation effect on HBV translation, transcription, and replication in SBHX21 cells[3].
Estradiol benzoate (0-500 μM, 72 h) has toxicity to Human foreskin fibroblasts (HFFs)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HFFs
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Concentration:0-500 μM
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Incubation Time:72 h
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Result:Inhibited HFFs at the 50% toxicity of 34.11 μM.
Estradiol benzoate (0.015-15000 μg/kg, Subcutaneous injection, single dose) advances male puberty in male Sprague-Dawley (SD) rats[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ovx mice[1]
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Dosage:20, 100 or 200 μg/kg
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Administration:Subcutaneous injection (s.c.)
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Result:Significantly reversed the lowered uterine weight induced by Ovx. Had no obvious effects on locomotor activity in Ovx mice. Effectively reversed the changes of enlarging the width of synaptic cleft and reducing the thickness of PSD induced by Ovx. Increased the number and density of synaptic vesicles by 4-week replacement with EB (100 μg/kg).
Chemical Information
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CAS No. 50-50-0
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Appearance Solid
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Masse moléculaire 376.49
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Formule C25H28O3
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Color White to off-white
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SMILES
C[C@@]12[C@@H](O)CC[C@@]1([H])[C@]3([H])CCC4=C(C=CC(OC(C5=CC=CC=C5)=O)=C4)[C@@]3([H])CC2
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Synonyms
β-Estradiol 3-benzoate; 17β-Estradiol 3-benzoate
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (17)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Highly specific neutrophil-mediated delivery of albumin nanoparticles to ectopic lesion for endometriosis therapy. [Abstract]2023 Mar 8;21(1):81. PMID: 36890521
Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Mar 8;21(1):81. [Abstract]
Estradiol benzoate (3 μg) induced endometriosis-like lesions in diverse peritoneal locations of female mice.
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Redox Biol
Stromal cell-derived itaconate promotes endometriosis via macrophage NRF2 and lysosomal pH modulation. [Abstract]2026 May:92:104101. PMID: 41780193 -
Phytomedicine
Cryptotanshinone alleviates immunosuppression in endometriosis by targeting MDSCs through JAK2/STAT3 pathway. [Abstract]2024 Nov 14:136:156227. PMID: 39580997
Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227. [Abstract]
Estradiol benzoate (0.3 μg; i.m.;every three days). H&E staining of the vehicle group revealed endometrial epithelial cells and stromal cells within the endometriotic lesions, along with glandular hyperplasia in the lamina propria.
Estradiol benzoate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Nov 14:136:156227. [Abstract]
Morphology of endometriotic lesions excised from the peritoneum of mice. Estradiol benzoate (0.3 μg; i.m.;every three days) successfully induced EMS in mice.
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J Med Chem
2024 Sep 12;67(17):15387-15410. PMID: 39159497
Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Med Chem. 2024 Sep 12;67(17):15387-15410. [Abstract]
Estradiol benzoate (17β-Estradiol) (1 μM; drinking water) was used to sustain the MCF7 xenograft model in NCG mice.
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Anal Chem
High-Throughput Reactive Desorption Electrospray Ionization Mass Spectrometry for Targeted Derivatization and Analysis of Poorly Ionized Small Molecules. [Abstract]2026 Jun 16;98(23):16962-16970. PMID: 42223360 -
J Ethnopharmacol
An experimental study of the anti-dysmenorrhea effect of Chinese herbal medicines used in Jin Gui Yao Lue. [Abstract]2019 Dec 5:245:112181. PMID: 31446072
Estradiol benzoate purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2019 Dec 5:245:112181. [Abstract]
ICR female mice were treated with Estradiol benzoate (1 mg/kg; i.p.; 3 d). On the fourth day, the mice were injected with oxytocin (50 U/kg) to induce dysmenorrhea.
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Bioorg Chem
Dehydroepiandrosterone ameliorates primary dysmenorrhea by suppressing the SP1/Hsp90ab1/COX-2 signaling pathway. [Abstract]2025 Feb 1:156:108235. PMID: 39914036 -
Molecules
Identification of Estradiol Benzoate as an Inhibitor of HBx Using Inducible Stably Transfected HepG2 Cells Expressing HiBiT Tagged HBx. [Abstract]2022 Aug 6;27(15):5000. PMID: 35956950 -
Front Cell Dev Biol
Comprehensive Analysis of Quantitative Proteomics With DIA Mass Spectrometry and ceRNA Network in Intrahepatic Cholestasis of Pregnancy. [Abstract]2022 Jul 22:10:854425. PMID: 35938169 -
iScience
Analysis of microbiota reveals the underlying mechanism of PHF11 in the development of Enterococcus-regulated endometriotic cysts. [Abstract]2023 Oct 7;26(11):108158. PMID: 37915596 -
Reprod Biomed Online
Blocking sphingosine 1-phosphate receptor 1 with modulators reduces immune cells infiltration and alleviates endometriosis in mice. [Abstract]2023 Nov;47(5):103304. PMID: 37757611 -
Mol Med Rep
Upregulation of miR‑33b promotes endometriosis via inhibition of Wnt/β‑catenin signaling and ZEB1 expression. [Abstract]2019 Mar;19(3):2144-2152. PMID: 30664209 -
J Pharmacol Sci
Activation of σ-1 receptor mitigates estrogen withdrawal-induced anxiety/depressive-like behavior in mice via restoration of GABA/glutamate signaling and neuroplasticity in the hippocampus. [Abstract]2024 Apr;154(4):236-245. PMID: 38485341 -
Am J Transl Res
2022 Apr 15;14(4):2184-2198. PMID: 35559378 -
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Evid Based Complement Alternat Med
Huayu Jiedu Fang Protects Ovarian Function in Mouse with Endometriosis Iron Overload by Inhibiting Ferroptosis. [Abstract]2022 Aug 30;2022:1406820. PMID: 36082180
Solvant et solubilité
DMSO : 50 mg/mL (132.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 3.33 mg/mL (8.84 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (280 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Portuguese - PT (419 KB)
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Instruction de manipulation (2659 KB)
Références
[6]. Zovko M, et al. Macromolecular prodrugs XI. Synthesis and characterization of polymer-estradiol conjugate. Int J Pharm. 2004 Nov 5;285(1-2):35-41. [Content Brief]
[7]. García-Gómez E, et al. Role of sex steroid hormones in bacterial-host interactions. Biomed Res Int. 2013;2013:928290. [Content Brief]
[8]. Estradiol benzoate
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| Ethanol / DMSO | 1 mM | 2.6561 mL | 13.2806 mL | 26.5611 mL | 66.4028 mL |
| 5 mM | 0.5312 mL | 2.6561 mL | 5.3122 mL | 13.2806 mL | |
| DMSO | 10 mM | 0.2656 mL | 1.3281 mL | 2.6561 mL | 6.6403 mL |
| 15 mM | 0.1771 mL | 0.8854 mL | 1.7707 mL | 4.4269 mL | |
| 20 mM | 0.1328 mL | 0.6640 mL | 1.3281 mL | 3.3201 mL | |
| 25 mM | 0.1062 mL | 0.5312 mL | 1.0624 mL | 2.6561 mL | |
| 30 mM | 0.0885 mL | 0.4427 mL | 0.8854 mL | 2.2134 mL | |
| 40 mM | 0.0664 mL | 0.3320 mL | 0.6640 mL | 1.6601 mL | |
| 50 mM | 0.0531 mL | 0.2656 mL | 0.5312 mL | 1.3281 mL | |
| 60 mM | 0.0443 mL | 0.2213 mL | 0.4427 mL | 1.1067 mL | |
| 80 mM | 0.0332 mL | 0.1660 mL | 0.3320 mL | 0.8300 mL | |
| 100 mM | 0.0266 mL | 0.1328 mL | 0.2656 mL | 0.6640 mL |
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