Cat. No.: HY-N2005

Cycleanine

Customer Review

Based on 1 Customer Validation

Cycleanine is a potent vascular selective Calcium antagonist. Cycleanine has analgesic, muscle relaxant and anti-inflammatory activities. Cycleanine has potential for anti-ovarian cancer acting through the apoptosis pathway.

For research use only. We do not sell to patients.

Purity: 99.80%
CAS No.: 518-94-5
Formula: C₃₈H₄₂N₂O₆
Molecular Weight:622.75

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Cycleanine Chemical Structure

CAS No.: 518-94-5

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

L-type calcium channel

All Calcium Channel Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	7.6 μM Compound: 1	Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay Antiproliferative activity against human A2780 cells after 72 hrs by SRB assay	[PMID: 29588214]
IGROV-1	IC₅₀	14 μM Compound: 1	Antiproliferative activity against human IGROV1 cells after 72 hrs by SRB assay Antiproliferative activity against human IGROV1 cells after 72 hrs by SRB assay	[PMID: 29588214]
OVCAR-8	IC₅₀	10 μM Compound: 1	Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay Antiproliferative activity against human OVCAR8 cells after 72 hrs by SRB assay	[PMID: 29588214]

In Vitro

Cycleanine inhibits L-type Ca-current (ICaL) of single rat ventricular cardiomyocytes in a voltage- and frequency-dependent manner^[1].
Cycleanine shows modestly less potency against human OSE cells (normal) than the cancer cells^[2].
Cycleanine (20 μM; 48 hours) exhibits cytotoxicity for Ovcar-8, A2780, Igrov-1, and Ovcar-4 cell lines with IC₅₀s ranging from 7 to 14 μM^[2].
Cycleanine (20 μM; 24 hours) results in significant PARP cleavage (a marker of apoptosis)^[2].
Cycleanine (20 μM; 48 hours) causes a significant increase of the population of both early and late apoptotic cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

Ovcar-8 cells, A2780 cells, Igrov-1 cells, Ovcar-4 cells
Concentration:

20 μM
Incubation Time:

48 hours
Result:

Exhibited cytotoxicity for Ovcar-8, A2780, Igrov-1, and Ovcar-4 cell lines with IC₅₀ values of 10 μM, 7.6 μM, 14 μM, 7.2 μM, respectively.

Western Blot Analysis^[2]

Cell Line:

Ovcar-8 cells
Concentration:

20 μM
Incubation Time:

24 hours
Result:

Induced 1.1-fold increase in PARP-1 cleavage compared with carboplatin.

Apoptosis Analysis^[2]

Cell Line:

Ovcar-8 cells
Concentration:

20 μM
Incubation Time:

48 hours
Result:

Caused a significant increase of the population of both early and late apoptotic cells.

Cell Cycle Analysis^[2]

Cell Line:

Ovcar-8 cells
Concentration:

20 μM
Incubation Time:

48 hours
Result:

Increased the percentage of Ovcar-8 cells in subG1.

In Vivo

Cycleanine inhibits the KCl-induced contraction of rabbit aortic rings with an IC₅₀ of 0.8 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 518-94-5
Appearance Solid
Molecular Weight 622.75
Formula C₃₈H₄₂N₂O₆
Color White to off-white
SMILES

COC1=C(OC2=CC=C(C[C@](N(CC3)C)([H])C4=C3C=C5OC)C=C2)C([C@@](N6C)([H])CC7=CC=C(OC4=C5OC)C=C7)=C(CC6)C=C1OC
Structure Classification
- Alkaloids
- Alkaloid Dimers
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (8.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6058 mL

8.0289 mL

16.0578 mL

5 mM

0.3212 mL

1.6058 mL

3.2116 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Martínez JA, et al. Calcium antagonist properties of the bisbenzylisoquinoline alkaloid cycleanine. Fundam Clin Pharmacol. 1998;12(2):182-7. [Content Brief]

[2]. Uche FI, et al. Cytotoxicity Effects and Apoptosis Induction by Bisbenzylisoquinoline Alkaloids from Triclisia subcordata. Phytother Res. 2016 Sep;30(9):1533-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6058 mL	8.0289 mL	16.0578 mL	40.1445 mL
DMSO	5 mM	0.3212 mL	1.6058 mL	3.2116 mL	8.0289 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Cycleanine

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Membrane Transporter/Ion Channel Compound Library

Neuronal Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Macrocyclic Compound Library

Anti-Cardiovascular Disease Compound Library

Traditional Chinese Medicine Active Compound Library

Neuroprotective Compound Library

Alkaloids Library

Anti-Cancer Natural Product Library

Protein-protein Interaction Inhibitor Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Calcium Channel Blocker Library

Antihypertensive Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Ion Channel Compound Library

Anti-Ovarian Cancer Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

All Calcium Channel Isoforms