dTAGV-1-NEG TFA
dTAGV-1-NEG TFA is an inactive FKBP12F36V PROTAC degrader, the diastereomer of dTAGV-1 (HY-145514D), and a heterobifunctional negative control molecule. dTAGV-1-NEG TFA does not degrade any protein.
(Pink: FKBP12 ligand (HY-114420); Blue: VHL ligand (HY-173627); Black: linker (HY-W012001)).
For research use only. We do not sell to patients.
- Formula: C70H91F3N6O16S
- Molecular Weight:1361.56
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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FKBP12 |
dTAGV-1-NEG (0.1 nM-10 μM; 24 h) TFA does not induce the degradation of FKBP12WT-Nluc or FKBP12F36V-Nluc in 293FT cells[1].
dTAGV-1-NEG (500 nM; 1-24 h) TFA does not induce the degradation of FKBP12F36V-KRASG12V in PATU-8902 FKBP12F36V-KRASG12V and KRAS-/- cells, nor does it alter the related signaling pathways[1].
dTAGV-1-NEG (500 nM; 4 h) TFA does not induce significant degradation of any proteins, including LACZ-FKBP12F36V, in PATU-8902 LACZ-FKBP12F36V cells[1].
dTAGV-1-NEG (0.01 nM-10 μM; 120 h) TFA does not reduce the viability of PATU-8902 FKBP12F36V-KRASG12V and KRAS-/- 3D spheroids[1].
dTAGV-1-NEG (1 μM; 24 h) TFA does not induce the degradation of FKBP12F36V-GFP or FKBP12F36V-EWS/FLI, nor does it alter the expression levels of EWS/FLI target proteins in the EWS502 cell line[1].
dTAGV-1-NEG (1 μM; 8 days) TFA does not inhibit the growth of EWS502 FKBP12F36V-EWS/FLI and EWS/FLI-/- cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PATU-8902 FKBP12F36V-KRASG12V, PATU-8902 KRAS-/-
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Concentration:500 nM
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Incubation Time:1 h, 2 h, 4 h, 8 h, 24 h
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Result:Did not induce degradation of FKBP12F36V-KRASG12V, nor alter levels of downstream signaling proteins pERK1/2, ERK1/2, pAKT S473, or AKT, compared to DMSO-treated controls across all tested time points.
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Cell Line:EWS502 FKBP12F36V-GFP, EWS502 FKBP12F36V-EWS/FLI, EWS502 EWS/FLI-/-
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Concentration:1 μM
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Incubation Time:24 h
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Result:Did not induce degradation of FKBP12F36V-GFP or FKBP12F36V-EWS/FLI, nor alter levels of the EWS/FLI downstream target NKX2-2, compared to DMSO-treated controls.
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Cell Line:EWS502 FKBP12F36V-EWS/FLI, EWS502 EWS/FLI-/-
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Concentration:1 μM
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Incubation Time:up to 8 days
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Result:Did not inhibit growth of cells, with growth rates matching those of DMSO-treated controls over the 8-day period.
Chemical Information
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Molecular Weight 1361.56
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Formula C70H91F3N6O16S
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SMILES
O=C([C@H]1N(C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)CCCC1)O[C@@H](C3=CC=CC=C3OCC(NCCCCCCC(N[C@@H](C(C)(C)C)C(N4[C@@H](C(N[C@H](C5=CC=C(C6=C(C)N=CS6)C=C5)C)=O)C[C@H](O)C4)=O)=O)=O)CCC7=CC=C(OC)C(OC)=C7.OC(C(F)(F)F)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
dTAGV-1-NEG TFA Related Classifications
HY-145514B Related Classifications
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