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Art. -Nr.: HY-145514B

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dTAGV-1-NEG TFA

dTAGV-1-NEG TFA is an inactive FKBP12^F36V PROTAC degrader, the diastereomer of dTAGV-1 (HY-145514D), and a heterobifunctional negative control molecule. dTAGV-1-NEG TFA does not degrade any protein.
(Pink: FKBP12 ligand (HY-114420); Blue: ligand (HY-173627); Black: linker (HY-W012001)).

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Formel: C₇₀H₉₁F₃N₆O₁₆S
Molecular Weight:1361.56

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (275 KB)

dTAGV-1-NEG TFA Chemical Structure

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Other In-stock Forms of dTAGV-1-NEG TFA

dTAGV-1-NEG

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

dTAGV-1-NEG TFA is an inactive FKBP12^F36V PROTAC degrader, the diastereomer of dTAGV-1 (HY-145514D), and a heterobifunctional negative control molecule. dTAGV-1-NEG TFA does not degrade any protein^[1]. (Pink: FKBP12 ligand (HY-114420); Blue: ligand (HY-173627); Black: linker (HY-W012001)).

IC₅₀ & Target

FKBP12

Alle PROTACs Isoform-spezifische Produkte anzeigen :

Alle FKBP Isoform-spezifische Produkte anzeigen :

In Vitro

dTAGV-1-NEG (0.1 nM-10 μM; 24 h) does not induce the degradation of FKBP12^WT-Nluc or FKBP12^F36V-Nluc in 293FT cells^[1].
dTAGV-1-NEG (500 nM; 1-24 h) does not induce the degradation of FKBP12^F36V-KRAS^G12V in PATU-8902 FKBP12^F36V-KRAS^G12V and KRAS^-/- cells, nor does it alter the related signaling pathways^[1].
dTAGV-1-NEG (500 nM; 4 h) does not induce significant degradation of any proteins, including LACZ-FKBP12^F36V, in PATU-8902 LACZ-FKBP12^F36V cells^[1].
dTAGV-1-NEG (0.01 nM-10 μM; 120 h) does not reduce the viability of PATU-8902 FKBP12^F36V-KRAS^G12V and KRAS^-/- 3D spheroids^[1].
dTAGV-1-NEG (1 μM; 24 h) does not induce the degradation of FKBP12^F36V-GFP or FKBP12^F36V-EWS/FLI, nor does it alter the expression levels of EWS/FLI target proteins in the EWS502 cell line^[1].
dTAGV-1-NEG (1 μM; 8 days) does not inhibit the growth of EWS502 FKBP12^F36V-EWS/FLI and EWS/FLI^-/- cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

PATU-8902 FKBP12^F36V-KRAS^G12V, PATU-8902 KRAS^-/-
Concentration:

500 nM
Incubation Time:

1 h, 2 h, 4 h, 8 h, 24 h
Result:

Did not induce degradation of FKBP12^F36V-KRAS^G12V, nor alter levels of downstream signaling proteins pERK1/2, ERK1/2, pAKT S473, or AKT, compared to DMSO-treated controls across all tested time points.

Western Blot Analysis^[1]

Cell Line:

EWS502 FKBP12^F36V-GFP, EWS502 FKBP12^F36V-EWS/FLI, EWS502 EWS/FLI^-/-
Concentration:

1 μM
Incubation Time:

24 h
Result:

Did not induce degradation of FKBP12^F36V-GFP or FKBP12^F36V-EWS/FLI, nor alter levels of the EWS/FLI downstream target NKX2-2, compared to DMSO-treated controls.

Cell Viability Assay^[1]

Cell Line:

EWS502 FKBP12^F36V-EWS/FLI, EWS502 EWS/FLI^-/-
Concentration:

1 μM
Incubation Time:

up to 8 days
Result:

Did not inhibit growth of cells, with growth rates matching those of DMSO-treated controls over the 8-day period.

Chemical Information

Molecular Weight 1361.56
Formel C₇₀H₉₁F₃N₆O₁₆S
SMILES

O=C([C@H]1N(C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)CCCC1)O[C@@H](C3=CC=CC=C3OCC(NCCCCCCC(N[C@@H](C(C)(C)C)C(N4[C@@H](C(N[C@H](C5=CC=C(C6=C(C)N=CS6)C=C5)C)=O)C[C@H](O)C4)=O)=O)=O)CCC7=CC=C(OC)C(OC)=C7.OC(C(F)(F)F)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Nabet B, et al. Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nat Commun. 2020 Sep 18;11(1):4687. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise

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