dTAGV-1-NEG
Based on 1 Customer Validation
dTAGV-1-NEG is an inactive FKBP12F36V PROTAC degrader, the diastereomer of dTAGV-1 (HY-145514D), and a heterobifunctional negative control molecule. dTAGV-1-NEG does not degrade any protein.
(Pink: FKBP12 ligand (HY-114420); Blue: VHL ligand (HY-173627); Black: linker (HY-W012001)).
For research use only. We do not sell to patients.
- Purity: 99.73%
- CAS No.: 2451573-87-6
- Formula: C68H90N6O14S
- Molecular Weight:1247.54
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All PROTACs Isoforms
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Biological Activity
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FKBP12 |
dTAGV-1-NEG (0.1 nM-10 μM; 24 h) does not induce the degradation of FKBP12WT-Nluc or FKBP12F36V-Nluc in 293FT cells[1].
dTAGV-1-NEG (500 nM; 1-24 h) does not induce the degradation of FKBP12F36V-KRASG12V in PATU-8902 FKBP12F36V-KRASG12V and KRAS-/- cells, nor does it alter the related signaling pathways[1].
dTAGV-1-NEG (500 nM; 4 h) does not induce significant degradation of any proteins, including LACZ-FKBP12F36V, in PATU-8902 LACZ-FKBP12F36V cells[1].
dTAGV-1-NEG (0.01 nM-10 μM; 120 h) does not reduce the viability of PATU-8902 FKBP12F36V-KRASG12V and KRAS-/- 3D spheroids[1].
dTAGV-1-NEG (1 μM; 24 h) does not induce the degradation of FKBP12F36V-GFP or FKBP12F36V-EWS/FLI, nor does it alter the expression levels of EWS/FLI target proteins in the EWS502 cell line[1].
dTAGV-1-NEG (1 μM; 8 days) does not inhibit the growth of EWS502 FKBP12F36V-EWS/FLI and EWS/FLI-/- cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PATU-8902 FKBP12F36V-KRASG12V, PATU-8902 KRAS-/-
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Concentration:500 nM
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Incubation Time:1 h, 2 h, 4 h, 8 h, 24 h
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Result:Did not induce degradation of FKBP12F36V-KRASG12V, nor alter levels of downstream signaling proteins pERK1/2, ERK1/2, pAKT S473, or AKT, compared to DMSO-treated controls across all tested time points.
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Cell Line:EWS502 FKBP12F36V-GFP, EWS502 FKBP12F36V-EWS/FLI, EWS502 EWS/FLI-/-
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Concentration:1 μM
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Incubation Time:24 h
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Result:Did not induce degradation of FKBP12F36V-GFP or FKBP12F36V-EWS/FLI, nor alter levels of the EWS/FLI downstream target NKX2-2, compared to DMSO-treated controls.
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Cell Line:EWS502 FKBP12F36V-EWS/FLI, EWS502 EWS/FLI-/-
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Concentration:1 μM
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Incubation Time:up to 8 days
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Result:Did not inhibit growth of cells, with growth rates matching those of DMSO-treated controls over the 8-day period.
Chemical Information
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CAS No. 2451573-87-6
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Appearance Solid
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Molecular Weight 1247.54
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Formula C68H90N6O14S
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Color White to off-white
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SMILES
O=C([C@H]1N(C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)CCCC1)O[C@@H](C3=CC=CC=C3OCC(NCCCCCCC(N[C@@H](C(C)(C)C)C(N4[C@@H](C(N[C@H](C5=CC=C(C6=C(C)N=CS6)C=C5)C)=O)C[C@H](O)C4)=O)=O)=O)CCC7=CC=C(OC)C(OC)=C7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (80.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.8016 mL | 4.0079 mL | 8.0158 mL | 20.0394 mL |
| 5 mM | 0.1603 mL | 0.8016 mL | 1.6032 mL | 4.0079 mL | |
| 10 mM | 0.0802 mL | 0.4008 mL | 0.8016 mL | 2.0039 mL | |
| 15 mM | 0.0534 mL | 0.2672 mL | 0.5344 mL | 1.3360 mL | |
| 20 mM | 0.0401 mL | 0.2004 mL | 0.4008 mL | 1.0020 mL | |
| 25 mM | 0.0321 mL | 0.1603 mL | 0.3206 mL | 0.8016 mL | |
| 30 mM | 0.0267 mL | 0.1336 mL | 0.2672 mL | 0.6680 mL | |
| 40 mM | 0.0200 mL | 0.1002 mL | 0.2004 mL | 0.5010 mL | |
| 50 mM | 0.0160 mL | 0.0802 mL | 0.1603 mL | 0.4008 mL | |
| 60 mM | 0.0134 mL | 0.0668 mL | 0.1336 mL | 0.3340 mL | |
| 80 mM | 0.0100 mL | 0.0501 mL | 0.1002 mL | 0.2505 mL |
dTAGV-1-NEG Related Classifications
HY-145514A Related Classifications
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