2. PROTAC Anti-infection
  3. PROTACs Influenza Virus SARS-CoV CMV
  4. FM-74-103

FM-74-103 is a selective GSPT1 PROTAC degrader and broad-spectrum antiviral agent. FM-74-103 recruits GSPT1 to the Cereblon E3 ligase to form a ternary complex, thereby driving GSPT1 ubiquitination and proteasomal degradation. FM-74-103 inhibits the replication of IAV, SARS-CoV-2 and CMV (including Nucleozin (HY-50001)-resistant influenza A virus). FM-74-103 can be used in research related to influenza A virus infection, SARS-CoV-2 infection and cytomegalovirus infection.
(Pink: GSPT1 ligand (HY-50001); Blue: Cereblon ligand (HY-138793); Black: linker).

For research use only. We do not sell to patients.

FM-74-103

FM-74-103 Chemical Structure

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FM-74-103 Related Antibodies

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von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

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Description

FM-74-103 is a selective GSPT1 PROTAC degrader and broad-spectrum antiviral agent. FM-74-103 recruits GSPT1 to the Cereblon E3 ligase to form a ternary complex, thereby driving GSPT1 ubiquitination and proteasomal degradation. FM-74-103 inhibits the replication of IAV, SARS-CoV-2 and CMV (including Nucleozin (HY-50001)-resistant influenza A virus). FM-74-103 can be used in research related to influenza A virus infection, SARS-CoV-2 infection and cytomegalovirus infection[1][2][3]. (Pink: GSPT1 ligand (HY-50001); Blue: Cereblon ligand (HY-138793); Black: linker).

IC50 & Target[1]

Cereblon

 

In Vitro

FM-74-103 selectively degrades GSPT1 in cells and potently inhibits the replication of various viruses, including Nucleozin-resistant IAV, SARS-CoV-2 in A549-ACE2 cells, and CMV, with extremely low cytotoxicity[1].
FM-74-103 (1 μM) reduces the levels of all SARS-CoV-2 viral proteins in infected A549-ACE2 cells and downregulates the expression of GSPT1 and eRF1[2].
FM-74-103 (1 μM; 3 h pre-infection and 24 h post infection) inhibits the replication of AD169BADrUL131 virus in ARPE-19 cells by degrading GSPT1/eRF1 and inducing eIF2α phosphorylation, with no significant cytotoxicity[2].
FM-74-103 (1 μM; 3 h pre-infection and 24 h post infection) inhibits SARS-CoV-2 replication in 3D lung organoids, which is confirmed by the reduction in SARS-CoV-2 NP levels[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FM-74-103 Related Antibodies

Cell Viability Assay[2]

Cell Line: ARPE-19 cells infected with AD169BADrUL131 (GFP-expressing CMV) virus
Concentration: 1 μM
Incubation Time: 3 h pre-infection and 24 h post infection
Result: Inhibit AD169BADrUL131 virus replication (reduced GFP fluorescence) in cells strongly.
Not reduce cell viability significantly.
Degrade GSPT1/eRF1 complex.
Induce integrated stress response (ISR) via phosphorylation of eIF2α, while total eIF2α levels remain constant.
Molecular Weight

755.22

Formula

C39H39ClN6O8
SMILES

ClC1=CC([N+]([O-])=O)=CC=C1N2CCN(CC2)C(C3=C(ON=C3C4=C(C=CC=C4)OCCCCCC5=CC=C6C(CN(C6=O)C7C(NC(CC7)=O)=O)=C5)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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FM-74-103 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FM-74-103PROTACsInfluenza VirusSARS-CoVCMVSARS coronavirusCytomegalovirusARPE-19 cellscytomegalovirusA549-ACE2 cellseRF1influenza A viruseIF2αSARS-CoV-2NHDF cellscereblon E3 ligaseGSPT1Inhibitorinhibitorinhibit

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FM-74-103
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HY-184248
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