FM-74-103
FM-74-103 is a selective GSPT1 PROTAC degrader and broad-spectrum antiviral agent. FM-74-103 recruits GSPT1 to the Cereblon E3 ligase to form a ternary complex, thereby driving GSPT1 ubiquitination and proteasomal degradation. FM-74-103 inhibits the replication of IAV, SARS-CoV-2 and CMV (including Nucleozin (HY-50001)-resistant influenza A virus). FM-74-103 can be used in research related to influenza A virus infection, SARS-CoV-2 infection and cytomegalovirus infection.
(Pink: GSPT1 ligand (HY-50001); Blue: Cereblon ligand (HY-138793); Black: linker).
For research use only. We do not sell to patients.
- Formula: C39H39ClN6O8
- Molecular Weight:755.22
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cereblon |
FM-74-103 selectively degrades GSPT1 in cells and potently inhibits the replication of various viruses, including Nucleozin-resistant IAV, SARS-CoV-2 in A549-ACE2 cells, and CMV, with extremely low cytotoxicity[1].
FM-74-103 (1 μM) reduces the levels of all SARS-CoV-2 viral proteins in infected A549-ACE2 cells and downregulates the expression of GSPT1 and eRF1[2].
FM-74-103 (1 μM; 3 h pre-infection and 24 h post infection) inhibits the replication of AD169BADrUL131 virus in ARPE-19 cells by degrading GSPT1/eRF1 and inducing eIF2α phosphorylation, with no significant cytotoxicity[2].
FM-74-103 (1 μM; 3 h pre-infection and 24 h post infection) inhibits SARS-CoV-2 replication in 3D lung organoids, which is confirmed by the reduction in SARS-CoV-2 NP levels[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ARPE-19 cells infected with AD169BADrUL131 (GFP-expressing CMV) virus
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Concentration:1 μM
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Incubation Time:3 h pre-infection and 24 h post infection
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Result:Inhibit AD169BADrUL131 virus replication (reduced GFP fluorescence) in cells strongly.
Not reduce cell viability significantly.
Degrade GSPT1/eRF1 complex.
Induce integrated stress response (ISR) via phosphorylation of eIF2α, while total eIF2α levels remain constant.
Chemical Information
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Molecular Weight 755.22
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Formula C39H39ClN6O8
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SMILES
ClC1=CC([N+]([O-])=O)=CC=C1N2CCN(CC2)C(C3=C(ON=C3C4=C(C=CC=C4)OCCCCCC5=CC=C6C(CN(C6=O)C7C(NC(CC7)=O)=O)=C5)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Jin J, et al. PROTACs in Antivirals: Current Advancements and Future Perspectives. Molecules. 2025;30(16):3402. Published 2025 Aug 18. [Content Brief]
[2]. Zhao N, et al. Generation of host-directed and virus-specific antivirals using targeted protein degradation promoted by small molecules and viral RNA mimics. Cell Host Microbe. 2023;31(7):1154-1169.e10. [Content Brief]
[3]. An Q, et al. New strategies to enhance the efficiency and precision of drug discovery. Front Pharmacol. 2025;16:1550158. Published 2025 Feb 11. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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