Harringtonine
Based on 18 publication(s) in Google Scholar
Harringtonine is a natural Cephalotaxus alkaloid that inhibits protein synthesis. Harringtonine has anti-chikungunya virus (CHIKV) activities with an EC50 of 0.24 μM.
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- Pureza: 99.93%
- No. CAS: 26833-85-2
- Fòrmula: C28H37NO9
- Peso molecular:531.59
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) Harringtonine
More- Cell Res. 2024 Sep;34(9):648-660. [Abstract]
- Nat Metab. 2025 Apr 11. [Abstract]
- Nat Immunol. 2025 Jul;26(7):1127-1138. [Abstract]
- Nat Immunol. 2024 Jun;25(6):981-993. [Abstract]
- Cancer Res. 2025 Aug 8. [Abstract]
- Nat Commun. 2025 Oct 13;16(1):9075. [Abstract]
- Nat Commun. 2024 Aug 26;15(1):7061. [Abstract]
- Nucleic Acids Res. 2019 Apr 8;47(6):e33. [Abstract]
- Cell Death Differ. 2023 Jan;30(1):137-151. [Abstract]
- Proc Natl Acad Sci U S A. 2021 Feb 16;118(7):e2014457118. [Abstract]
- FASEB J. 2021 Jun;35(6):e21656. [Abstract]
- J Anim Sci. 2024 Aug 19:skae239. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- bioRxiv. 2026 May 19:2026.05.17.725791. [Abstract]
- Biomed Pharmacother. 2024 Jun 11:176:116907. [Abstract]
- bioRxiv. 2023 Jun 25.
- bioRxiv. 2023 Jan 17.
- bioRxiv. January 25, 2022.
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BHK-21 | EC50 |
0.24 μM
Compound: 22
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Antiviral activity against Chikungunya virus infected in BHK21 cells assessed as inhibition of viral replication after 24 hrs
Antiviral activity against Chikungunya virus infected in BHK21 cells assessed as inhibition of viral replication after 24 hrs
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[PMID: 24079775] |
| HeLa | IC50 |
4 μM
Compound: HT
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Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
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[PMID: 35148094] |
| HL-60 | IC50 |
0.047 μM
Compound: HT
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Antiproliferative activity against human HL60 cells after 62 hrs by MTS assay
Antiproliferative activity against human HL60 cells after 62 hrs by MTS assay
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[PMID: 29286665] |
| HL-60 | IC50 |
50.5 nM
Compound: HT
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Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
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[PMID: 35148094] |
| P388 | IC50 |
0.032 μg/mL
Compound: 3
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Antileukemic activity against P-388 leukemia cells.
Antileukemic activity against P-388 leukemia cells.
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10.1016/0960-894X(96)00302-2 |
Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit and therefore block aminoacyl-tRNA binding and peptide bond formation[1]. Harringtonine displays potent inhibition of Chikungunya virus infection with an EC50 of 0.24 μM. Harringtonine could inhibit other alphaviruses[2]. Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations. The mechanism of the antitumor action of harringtonine is considered to be an effect on protein synthesis and is characterized by breakdown of polysomes to monosomes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 26833-85-2
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Appearance Solid
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Peso molecular 531.59
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Fòrmula C28H37NO9
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Color White to light yellow
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SMILES
O=C([C@@](CC(OC)=O)(O)CCC(C)(O)C)O[C@H]1[C@](C2=CC(OCO3)=C3C=C2CCN4CCC5)([H])[C@]45C=C1OC
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (18)
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Journal Impact Factor
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Most Recent
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Cell Res
SMYD5 is a ribosomal methyltransferase that catalyzes RPL40 lysine methylation to enhance translation output and promote hepatocellular carcinoma. [Abstract]2024 Sep;34(9):648-660. PMID: 39103523 -
Nat Metab
NK cell-derived IFNγ mobilizes free fatty acids from adipose tissue to promote early B cell activation during viral infection. [Abstract]2025 Apr 11. PMID: 40217117 -
Nat Immunol
Cathepsin L-dependent positive selection shapes clonal composition and functional fitness of CD4+ T cells. [Abstract]2025 Jul;26(7):1127-1138. PMID: 40514418 -
Nat Immunol
An IFNγ-dependent immune-endocrine circuit lowers blood glucose to potentiate the innate antiviral immune response. [Abstract]2024 Jun;25(6):981-993. PMID: 38811816 -
Cancer Res
Caloric Restriction Enhances the Efficacy of Anti-Androgen Therapy in Prostate Cancer by Inhibiting Androgen Receptor Translation. [Abstract]2025 Aug 8. PMID: 40779415
Harringtonine purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Aug 8. [Abstract]
Cells were treated with 2 µg/ml harringtonine (HT) for 3 minutes before cell lysate preparation. The total transcript level for AR mRNA in each fraction was shown in the bar graphs and the percentage shift was calculated.
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Nat Commun
2025 Oct 13;16(1):9075. PMID: 41083459
Harringtonine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Oct 13;16(1):9075. [Abstract]
Ribosomal elongation speed was measured using harringtonine chase. Polysome profiles of CTL cells or A8-KO cells treated with harringtonine (2 μg/mL; 0–5 min) for indicated times were obtained.
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Nat Commun
2024 Aug 26;15(1):7061. PMID: 39187487
Harringtonine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 26;15(1):7061. [Abstract]
Harringtonine (2 μg/mL; 60–180 s). Smoothed metagene plots for a ribosome run-off assay with harringtonine (HAR) chase were generated.
Harringtonine purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 26;15(1):7061. [Abstract]
Harringtonine (2 μg/mL; 60–180 s).The expansion of the ribosome-free area over time by harringtonine chase. Linear regression revealed that the global elongation rate was 4.1 codon/s.
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Nucleic Acids Res
SpyCLIP: an easy-to-use and high-throughput compatible CLIP platform for the characterization of protein-RNA interactions with high accuracy. [Abstract]2019 Apr 8;47(6):e33. PMID: 30715466
Harringtonine purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2019 Apr 8;47(6):e33. [Abstract]
HAR: clusters identified upon acute translation suppression by Harringtonine (2 μg/ml; 1 h). Distribution of AGO2 SpyCLIP clusters in different regions of protein-coding genes and distribution of AGO2 SpyCLIP clusters in different genomic regions corrected by region size.
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Cell Death Differ
SOCS2-enhanced ubiquitination of SLC7A11 promotes ferroptosis and radiosensitization in hepatocellular carcinoma. [Abstract]2023 Jan;30(1):137-151. PMID: 35995846 -
Proc Natl Acad Sci U S A
2021 Feb 16;118(7):e2014457118. PMID: 33568529 -
FASEB J
C81-evoked inhibition of the TNFR1-NFκB pathway during inflammatory processes for stabilization of the impaired vascular endothelial barrier for leukocytes. [Abstract]2021 Jun;35(6):e21656. PMID: 34042211 -
J Anim Sci
Glucose inhibits the inflammatory response in goose fatty liver by increasing the ubiquitination level of PKA. [Abstract]2024 Aug 19:skae239. PMID: 39158360 -
Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
bioRxiv
2026 May 19:2026.05.17.725791. PMID: 42239437 -
Biomed Pharmacother
(Homo-)harringtonine prevents endothelial inflammation through IRF-1 dependent downregulation of VCAM1 mRNA expression and inhibition of cell adhesion molecule protein biosynthesis. [Abstract]2024 Jun 11:176:116907. PMID: 38865849 -
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Solvente y solubilidad
DMSO : 100 mg/mL (188.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.70 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
For harringtonine treatment studies with Sindbis virus, BHK21 cells are seeded into 96-well plates and infected with Sindbis virus at an MOI of 1 for 1 h prior to being washed twice with PBS and incubated with various concentrations of harringtonine (0.1 μM, 1 μM, 5 μM, and 10 μM) at 37°C with 5% CO2. Cell supernatants are harvested for plaque assays at 24 h postinfection[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (283 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Fresno M, et al. Inhibition of translation in eukaryotic systems by harringtonine. Eur J Biochem. 1977 Jan;72(2):323-30. [Content Brief]
[2]. Kaur P, et al. Inhibition of chikungunya virus replication by harringtonine, a novel antiviral that suppresses viral protein expression. Antimicrob Agents Chemother. 2013 Jan;57(1):155-67. [Content Brief]
[3]. Piao YF, et al. Growth inhibition of human myeloid leukemia cells in vitro by harringtonine. Gan To Kagaku Ryoho. 1990 Feb;17(2):281-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8811 mL | 9.4057 mL | 18.8115 mL | 47.0287 mL |
| 5 mM | 0.3762 mL | 1.8811 mL | 3.7623 mL | 9.4057 mL | |
| 10 mM | 0.1881 mL | 0.9406 mL | 1.8811 mL | 4.7029 mL | |
| 15 mM | 0.1254 mL | 0.6270 mL | 1.2541 mL | 3.1352 mL | |
| 20 mM | 0.0941 mL | 0.4703 mL | 0.9406 mL | 2.3514 mL | |
| 25 mM | 0.0752 mL | 0.3762 mL | 0.7525 mL | 1.8811 mL | |
| 30 mM | 0.0627 mL | 0.3135 mL | 0.6270 mL | 1.5676 mL | |
| 40 mM | 0.0470 mL | 0.2351 mL | 0.4703 mL | 1.1757 mL | |
| 50 mM | 0.0376 mL | 0.1881 mL | 0.3762 mL | 0.9406 mL | |
| 60 mM | 0.0314 mL | 0.1568 mL | 0.3135 mL | 0.7838 mL | |
| 80 mM | 0.0235 mL | 0.1176 mL | 0.2351 mL | 0.5879 mL | |
| 100 mM | 0.0188 mL | 0.0941 mL | 0.1881 mL | 0.4703 mL |
Harringtonine Related Classifications
HY-N0862 Related Classifications
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