RhoNox-4
Based on 1 Customer Validation
RhoNox-4 is a ferroptosis inhibitor and a fluorescence-activating indicator specific to Fe2+. RhoNox-4 selectively reacts with labile Fe2+ and oxidizes it to Fe3+, depleting labile Fe2+ involved in lipid peroxidation and inhibiting ferroptosis induced by ferroptosis inducers. RhoNox-4 is cell-permeable, accumulates in the endoplasmic reticulum and/or Golgi apparatus, and can be used for live-cell imaging as well as high-throughput screening of subcellular iron status modulators, making it applicable for iron biology research.
For research use only. We do not sell to patients.
- Purity: 98.65%
- CAS No.: 2711762-70-6
- Formula: C38H44N4O8
- Molecular Weight:684.78
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
RhoNox-4 (0.2-12.8 μM; 12 h) acts as a ferroptosis inhibitor in HT1080 cells treated with Erastin (HY-15763), maintaining approximately 80% cell viability at a concentration of 12.8 μM, with an IC50 of approximately 3.5 μM[1][4].
RhoNox-4 (0.2-12.8 μM; 12 h) significantly inhibits RSL-3 (HY-100218A)-induced ferroptosis in HT1080 cells, and reduces the cell mortality rate by more than 50% at effective concentrations[4].
RhoNox-4 (2 μM; 60 minutes) enables efficient and selective detection of Fe2+[2] in a cell-free HEPES buffer system.
RhoNox-4 (1 μM) can effectively penetrate the cell membrane of HepG2 cells and enable live-cell imaging of labile Fe2+[1].
RhoNox-4 (1 μM; 30 minutes) enables efficient live-cell high-throughput screening (HTS) targeting iron homeostasis regulators[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT1080 human fibrosarcoma cells
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Concentration:0.2, 0.8, 3.2, 12.8 μM
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Incubation Time:12 h
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Result:Exhibited potent inhibition of erastin-induced ferroptosis, with an IC50 value of 3.5 μM.
Restored HT1080 cell viability to over 90% relative to control levels at 12.8 μM, with statistically significant protection compared to erastin-only treatment.\nDemonstrated robust inhibition of RSL-3-induced ferroptosis, reducing cell death by more than 50% across effective concentrations.
Restored HT1080 cell viability to approximately 60% relative to control levels at 12.8 μM, with statistically significant protection compared to RSL-3-only treatment.
Chemical Information
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CAS No. 2711762-70-6
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Appearance Solid
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Molecular Weight 684.78
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Formula C38H44N4O8
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Color Off-white to pink
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SMILES
CC(C)(OC(N1CC[N+](C2=CC3=C(C4(C5=CC=C(C=C5O3)N6CCN(C(OC(C)(C)C)=O)CC6)OC(C7=C4C=CC=C7)=O)C=C2)(CC1)[O-])=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (73.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (1.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hirayama T, et al. -oxide-based and trioxolane-based fluorescent probes for monitoring labile iron and labile heme: an update. Journal of clinical biochemistry and nutrition. 2026 Jan;78(1):6-13. [Content Brief]
[2]. Hirayama T, et al. High-Throughput Screening for the Discovery of Iron Homeostasis Modulators Using an Extremely Sensitive Fluorescent Probe. ACS sensors. 2020 Sep 25;5(9):2950-2958. [Content Brief]
[3]. Tabata R, et al. Systemic Regulation of Iron Acquisition by Arabidopsis in Environments with Heterogeneous Iron Distributions. Plant & cell physiology. 2022 Jun 15;63(6):842-854. [Content Brief]
[4]. Kawai K, et al. Inhibition of ferroptosis by -oxide-based fluorescent probes selective oxidation of ferrous ions. Chemical science. 2025 Jun 25;16(25):11240-11245. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4603 mL | 7.3016 mL | 14.6032 mL | 36.5081 mL |
| 5 mM | 0.2921 mL | 1.4603 mL | 2.9206 mL | 7.3016 mL | |
| 10 mM | 0.1460 mL | 0.7302 mL | 1.4603 mL | 3.6508 mL | |
| 15 mM | 0.0974 mL | 0.4868 mL | 0.9735 mL | 2.4339 mL | |
| 20 mM | 0.0730 mL | 0.3651 mL | 0.7302 mL | 1.8254 mL | |
| 25 mM | 0.0584 mL | 0.2921 mL | 0.5841 mL | 1.4603 mL | |
| 30 mM | 0.0487 mL | 0.2434 mL | 0.4868 mL | 1.2169 mL | |
| 40 mM | 0.0365 mL | 0.1825 mL | 0.3651 mL | 0.9127 mL | |
| 50 mM | 0.0292 mL | 0.1460 mL | 0.2921 mL | 0.7302 mL | |
| 60 mM | 0.0243 mL | 0.1217 mL | 0.2434 mL | 0.6085 mL |
RhoNox-4 Related Classifications
HY-D3116 Related Classifications
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