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IL-2 Protein, Human

24 Cited Publications
カスタマーレビュー

Based on 24 publication(s) in Google Scholar

IL-2 Protein, Human activates STAT, PI3K and MAPK pathways. IL-2 Protein, Human increases NK-cell cytolytic activity as a T-cell growth factor. IL-2 Protein, Human regulates the proliferation of activated B-cells and regulatory T-cells. IL-2 Protein, Human is the recombinant human-derived IL-2 Protein, expressed by E. coli.

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  • Species: Human
  • Source: E. coli
  • 保管条件:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-2 Protein, Human
  • Experimental Validation Results for IL-2 Protein, Human
  • IL-2 Protein, Human Product Image
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  • 生物活性
  • Technical Parameters
  • Product Properties
  • ドキュメント
  • 参考文献
  • Help & FAQs
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Use of MCE Products

生物活性

製品説明

IL-2 Protein, Human activates STAT, PI3K and MAPK pathways. IL-2 Protein, Human increases NK-cell cytolytic activity as a T-cell growth factor. IL-2 Protein, Human regulates the proliferation of activated B-cells and regulatory T-cells. IL-2 Protein, Human is the recombinant human-derived IL-2 Protein, expressed by E. coli.

Background

Recombinant human interleukin-2 (IL-2) expressed as Escherichia coli is isolated as insoluble aggregates of protein (inclusion bodies) after cell breakage. Interleukin 2 (IL-2), a cytokine secreted by activated T cells, promotes the differentiation and proliferation of lymphoid cells as well as enhances the cell-mediated immune response to infections. Therefore IL-2 has been used as an adjunctive immunotherapeutic agent to control some infectious diseases, such as leishmaniasis, leprosy, and HIV infection[1].

体外実験

IL-2 Protein (Human, 500 U/mL, 42 hours-5 days) induces cytotoxic activity in mouse splenocytes and human peripheral blood lymphocytes, which exhibits a killing effect on tumor cells[2].

Verified Bioactivity

1. The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
2. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells.The ED50 for this effect is <3.0 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • アクセッション番号

    P60568 (A21-T153)

  • 遺伝子ID
    3558  [NCBI]
  • Molecular Construction
    • N-term
    • IL-2 (A21-T153)
      Accession # P60568
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • 別名

    IL2; T Cell Growth Factor; Interleukin 2; Aldesleukin; IL-2; Involved In Regulation Of T-Cell Clonal Expansion; TCGF; T-Cell Growth Factor; Interleukin-2; Lymphokine

  • AA Sequence

    APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

  • Predicted Molecular Mass

    16.9 kDa

  • 分子量

    Approximately 14-16 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • 純度

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

性状

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 10 mM sodium citrate, pH 4.0.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM sodium citrate, pH 4.0, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

輸送条件

Room temperature in continental US; may vary elsewhere.

参考文献

[1]. Zhang R, et al. Therapeutic effects of recombinant human interleukin 2 as adjunctive immunotherapy against tuberculosis: A systematic review and meta-analysis. PLoS One. 2018 Jul 19;13(7):e0201025. [Content Brief]

[2]. Devos R, et al., Induction of cytolytic cells by pure recombinant human interleukin 2. Eur J Immunol. 1984 Nov;14(11):1057-60. [Content Brief]

Calculators

再構成計算機

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
希釈計算機

濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)

濃度 (開始) 濃度 (開始)
×
体積 (開始) 体積 (開始)
=
濃度 (終了) 濃度 (終了)
×
体積 (終了) 体積 (終了)
比活性計算機
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     The ED50 is 0.1 ng/mL as measured by CTLL-2 cells, corresponding to a specific activity of 1 × 107 units/mg.

  • IL-2 Protein, Human Product Image
    1/16
  • Experimental Validation Results for IL-2 Protein, Human
  • Experimental Validation Results for IL-2 Protein, Human
  • IL-2 Protein, Human Product Image