2. Academic Validation
  3. Nordentatin Inhibits Neuroblastoma Cell Proliferation and Migration through Regulation of GSK-3 Pathway

Nordentatin Inhibits Neuroblastoma Cell Proliferation and Migration through Regulation of GSK-3 Pathway

  • Curr Issues Mol Biol. 2022 Feb 24;44(3):1062-1074. doi: 10.3390/cimb44030070.
Chantana Boonyarat 1,  Panatchakorn Boonput 2,  Nantakorn Tongloh 2,  Rawiwun Kaewamatawong 2,  Suchada Chaiwiwatrakul 3,  Chavi Yenjai 4,  Pornthip Waiwut 2
Affiliations

Affiliations

  • 1 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
  • 2 Faculty of pharmaceutical sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
  • 3 Department of English, Faculty of Humanities and Social Sciences, Ubon Ratchathani Rajabhat University, Ubon Ratchathani 34000, Thailand.
  • 4 Natural Products Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
PMID: 35723293 DOI: 10.3390/cimb44030070
Abstract

Cancer is caused by abnormal cell changes leading to uncontrolled cell growth. The specific characteristics of Cancer cells, including the loss of apoptotic control and the ability to migrate into and invade the surrounding tissue, result in Cancer cell metastasis to Other parts of the body. Therefore, the inhibition of the proliferation, migration, and invasion of Cancer cells are the principal goals in the treatment of Cancer. This study aimed to investigate the inhibitory activity of nordentatin, a coumarin derivative isolated from Clausena harmandiana, regarding the proliferation and migration of human neuroblastoma cells (SH-SY5Y). Nordentatin at a concentration of 100 µM showed cell cytotoxicity toward SH-SY5Y that was significantly different from that of the control group (p < 0.01) at 24, 48, and 72 h. Moreover, nordentatin inhibited SH-SY5Y proliferation by inhibiting the antiapoptotic protein Mcl-1, leading to the cleavage of Caspase-3 and resulting in the inhibition of a migratory protein, MMP-9, through the GSK-3 pathway (compared with cells treated with a GSK inhibitor). These results suggest that nordentatin inhibited the proliferation and migration of neuroblastoma cells through the GSK-3 pathway.

Keywords

GSK-3; apoptosis; cell proliferation; migration; nordentatin.

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