1. Apoptosis
  2. Bcl-2 Family
  3. MIM1

MIM1 (Synonyms: Inhibitor of Mcl-1)

Cat. No.: HY-16695 Purity: >98.0%
Handling Instructions

MIM-1 is an inhibitor of myeloid cell factor 1 (Mcl-1).

For research use only. We do not sell to patients.

MIM1 Chemical Structure

MIM1 Chemical Structure

CAS No. : 509102-00-5

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10 mM * 1 mL in DMSO USD 69 In-stock
Estimated Time of Arrival: December 31
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Estimated Time of Arrival: December 31
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Description

MIM-1 is an inhibitor of myeloid cell factor 1 (Mcl-1).

IC50 & Target[1]

Mcl-1

 

In Vitro

MIM-1 selectively targets the BH3 binding groove of Mcl-1, with Bak-dependent apoptotic activity. To estimate the sensitivity of HA and T98G cells to the apoptosis inhibitor MIM-1, the colorimetric MTT assay is used to detect cell viability and to determine the IC50 value. The IC50 value of the HA cell line is almost 5-fold lower (16.10 µM) compared with the IC50 of the T98G cell line (80.20 µM) after MIM-1 inhibitor treatment.

Molecular Weight

347.43

Formula

C₁₇H₂₁N₃O₃S

CAS No.

509102-00-5

SMILES

OC1=C(O)C(/C=N/N2/C(SC=C2C)=N/C3CCCCC3)=CC=C1O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

References
Cell Assay
[1]

The IC50 value, defined as the concentration that reduces the global growth of cells by 50%, is determined for the apoptosis inhibitors ABT-737 and MIM-1, individually, for the human astrocyte (HA) and human GB (T98G) cell lines. The apoptosis inhibitor concentrations and treatment time periods are selected experimentally according to preliminary experiments. The final ABT-737 treatment is performed with 10-fold increasing concentrations in the range of 0.001-100 µM, and the final MIM-1 treatment is performed with 4-fold increasing concentrations in the range of 0.4-400 µM, for 48 h. The biochemical colorimetric MTT assay, based on the enzymatic conversion of MTT to a violet formazan salt, is used to assess the viability of the HA and T98G cells. Briefly, the cells in culture medium are seeded (3.5×103 cells/well for the HA cell line, 3.0×103 cells/well for T98G cell line) in 96-well microtiter plates. On the third day, the medium is changed to culture medium supplemented with the apoptosis inhibitor ABT-737 or MIM-1 at varied concentrations and incubation continued for another two days. After the treatment with the apoptosis inhibitors, cells are rinsed once with Dulbeccos phosphate buffer saline (DPBS) and further incubated in medium supplemented with 0.5 mg/mL MTT in a humidified atmosphere for 6 h. During a subsequent incubation for 16 h in medium containing SDS [5% (w/v)], the precipitated formazan, the amount of which is proportional to the number of live cells, is solubilized. The absorbance of the formazan-containing solution is measured at 540 nm using an ELISA plate reader. The absorbance is also determined for the medium of the control cells not exposed to the apoptosis inhibitors. The percentage of cell viability is calculated relative to the untreated control cells. The IC50 values are determined for both human brain cell lines after individual apoptosis inhibitor treatment.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

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Keywords:

MIM1Inhibitor of Mcl-1MIM 1MIM-1Inhibitor of Mcl1Inhibitor of Mcl 1Bcl-2 FamilyInhibitorinhibitorinhibit

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