2. Metabolic Enzyme/Protease Apoptosis
  3. Phosphodiesterase (PDE) Apoptosis
  4. Nauclefine

Nauclefine is an indole alkaloid isolated from Nauclea officinalis. Nauclefine acts as a PDE3A modulator to induce cancer cell apoptosis through a PDE3A-SLFN12-dependent death pathway.

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Nauclefine

Nauclefine Chemical Structure

CAS No. : 57103-51-2

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Based on 1 publication(s) in Google Scholar

Nauclefine Related Antibodies

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

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Description

Nauclefine is an indole alkaloid isolated from Nauclea officinalis. Nauclefine acts as a PDE3A modulator to induce cancer cell apoptosis through a PDE3A-SLFN12-dependent death pathway[1].

In Vitro

Nauclefine (250?nM, 48 h) can inhibit the viability of HeLa cells and induce apoptosis, resulting in cell death dependent on PDE3A and SLFN12. Nauclefine can bind PDE3A, but does not inhibit its phosphodiesterase activity, can promote the interaction between PDE3A and SLFN12, and promote cell death by increasing the stability of SLFN12[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nauclefine Related Antibodies
In Vivo

Nauclefine (5 mg/kg, intratumorally, once per day for 22 days) significantly reduces the overall growth of HeLa-luc cell tumors in female nude mice (Balb/c-nu) infected with HeLa cells, especially after treatment 6 Days later, the tumor volume is significantly reduced without causing any decrease in the body weight of the mice. Its inhibition of tumor growth depends on the expression of PDE3A and SLFN12[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

287.32

Formula

C18H13N3O
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1C2=C(C=C3C4=C(CCN13)C5=C(C=CC=C5)N4)C=CN=C2
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (69.61 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4804 mL 17.4022 mL 34.8044 mL
5 mM 0.6961 mL 3.4804 mL 6.9609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.65%

SDS (393 KB)

COA (252 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4804 mL 17.4022 mL 34.8044 mL 87.0110 mL
5 mM 0.6961 mL 3.4804 mL 6.9609 mL 17.4022 mL
10 mM 0.3480 mL 1.7402 mL 3.4804 mL 8.7011 mL
15 mM 0.2320 mL 1.1601 mL 2.3203 mL 5.8007 mL
20 mM 0.1740 mL 0.8701 mL 1.7402 mL 4.3505 mL
25 mM 0.1392 mL 0.6961 mL 1.3922 mL 3.4804 mL
30 mM 0.1160 mL 0.5801 mL 1.1601 mL 2.9004 mL
40 mM 0.0870 mL 0.4351 mL 0.8701 mL 2.1753 mL
50 mM 0.0696 mL 0.3480 mL 0.6961 mL 1.7402 mL
60 mM 0.0580 mL 0.2900 mL 0.5801 mL 1.4502 mL
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Nauclefine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nauclefine57103-51-2Phosphodiesterase (PDE)ApoptosisNauclea officinalisPDE3AapoptosiscancerInhibitorinhibitorinhibit

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