NH4-6 

NH4-6 is a SIRT2 inhibitor with an IC₅₀ of 0.032 μM against SIRT2 and an IC₅₀ of 3 μM against SIRT1. NH4-6 inhibits the deacetylase activity of SIRT1. As a cytotoxic agent, NH4-6 reduces cancer cell viability, suppresses anchorage-independent growth of cancer cells, induces acetylation of α-tubulin, and promotes acetylation of p53. NH4-6 can be used in the research of breast cancer^[1].

NH4-6 potently and selectively inhibits purified SIRT2 deacetylase activity with an IC₅₀ of 0.032 μM, shows weaker activity against SIRT1 and SIRT6, and does not inhibit SIRT3 at 83 μM^[1].
NH4-6 (25-50 μM; 6 h for permeability) exhibits weaker cytotoxicity than TM at 25 μM but stronger cytotoxicity at 50 μM in MCF7 and MDA-MB-231 breast cancer cells, correlating with its concentration-dependent cellular uptake enabled by superior aqueous solubility^[1].
NH4-6 (12 μM; 10 days) shows stronger inhibition of anchorage-independent growth than TM at 12 μM in MCF7 breast cancer cells^[1].
NH4-6 (25-100 μM; 6 h) increases α-tubulin acetylation (a marker of SIRT2 inhibition) in MCF7 breast cancer cells at 50 μM and 100 μM, but not at 25 μM, consistent with its concentration-dependent cellular uptake^[1].
NH4-6 (10-100 μM) inhibits SIRT1 activity (measured via p53 acetylation) in MCF7 breast cancer cells only at a concentration of 100 μM, likely due to its improved solubility enabling sufficient cellular accumulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

718.82

C₃₃H₅₉IN₄O₃S

2375182-58-2

O=C(OCC1=CC=CC=C1)N[C@@H](CCCCNC(CCCCCCCCCCCCC)=S)C(NCC[N+](C)(C)C)=O.[I-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Hong JY, et al. A Glycoconjugated SIRT2 Inhibitor with Aqueous Solubility Allows Structure-Based Design of SIRT2 Inhibitors. ACS Chem Biol. 2019;14(8):1802-1810.