NH4-6

NH4-6 potently and selectively inhibits purified SIRT2 deacetylase activity with an IC₅₀ of 0.032 μM, shows weaker activity against SIRT1 and SIRT6, and does not inhibit SIRT3 at 83 μM^[1].
NH4-6 (25-50 μM; 6 h for permeability) exhibits weaker cytotoxicity than TM at 25 μM but stronger cytotoxicity at 50 μM in MCF7 and MDA-MB-231 breast cancer cells, correlating with its concentration-dependent cellular uptake enabled by superior aqueous solubility^[1].
NH4-6 (12 μM; 10 days) shows stronger inhibition of anchorage-independent growth than TM at 12 μM in MCF7 breast cancer cells^[1].
NH4-6 (25-100 μM; 6 h) increases α-tubulin acetylation (a marker of SIRT2 inhibition) in MCF7 breast cancer cells at 50 μM and 100 μM, but not at 25 μM, consistent with its concentration-dependent cellular uptake^[1].
NH4-6 (10-100 μM) inhibits SIRT1 activity (measured via p53 acetylation) in MCF7 breast cancer cells only at a concentration of 100 μM, likely due to its improved solubility enabling sufficient cellular accumulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.