NS-417 free base
NS-417 free base is an ERK1, ERK2, and Akt kinase activator with neuroprotective, neurite outgrowth potentiating, and dopaminergic cell population enhancing activity. NS-417 free base enhances activation of ERK1, ERK2, and Akt kinase via growth factor stimulation. NS-417 free base rescues cells from growth factor withdrawal-induced death, stimulates neurite outgrowth, increases tyrosine hydroxylase-positive cell counts, and displays neurotrophic-like activity in in vitro models. NS-417 free base can be used for the research of parkinson’s disease.
For research use only. We do not sell to patients.
- CAS No.: 160753-58-2
- Formula: C18H16ClN3O2
- Molecular Weight:341.79
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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ERK1 |
ERK2 |
Akt |
NS-417 (0.01-10 μM; 4 days) free base prevents death of differentiated rat PC12 pheochromocytoma cells deprived of serum and NGF, with maximal survival effects observed at 2-5 μM after 4 days of incubation[1].
NS-417 (1-3 μM; 2 days) free base induces short neurite-like processes in undifferentiated rat PC12 pheochromocytoma cells and significantly potentiates neurite outgrowth induced by NGF, bFGF, and EGF after 2 days of incubation[1].
NS-417 (1-3 μM; 24 h) free base potentiates long-term (24-hour) NGF-induced ERK1/ERK2 and Akt activation in rat PC12 pheochromocytoma cells, and at 3 μM (10 min) enhances short-term (10-minute) ERK1/ERK2 activation induced by NGF, bFGF, and EGF[1].
NS-417 (1 μM; 7 days) free base increases the number of tyrosine hydroxylase-positive dopaminergic neurons in dissociated E14 rat ventral mesencephalic cultures by 81% after 7 days of incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:differentiated rat PC12 pheochromocytoma cells
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Concentration:0.01 μM; 0.02 μM; 0.05 μM; 0.1 μM; 0.2 μM; 1 μM; 2 μM; 5 μM; 10 μM
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Incubation Time:4 days
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Result:Prevented cell loss in a dose-dependent manner.
Exerted maximal survival effect at 2-5 μM, comparable to 3 nM NGF.
Resulted in significantly higher cell viability at 1, 2, 5, and 10 μM compared to untreated cells.
Preserved the neurites of surviving cells.
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Cell Line:rat PC12 pheochromocytoma cells
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Concentration:1 μM; 3 μM
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Incubation Time:24 h; 10 min
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Result:Potentiated 24-hour NGF-induced activation of ERK1/ERK2 and phosphorylation of Akt (Ser-473).
Enhanced ERK1/ERK2 activation induced by 10 minutes of stimulation with NGF, bFGF, or EGF at 3 μM.
Chemical Information
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CAS No. 160753-58-2
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Molecular Weight 341.79
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Formula C18H16ClN3O2
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SMILES
O=C1NC2=C(/C1=N/O)C=C(C3=C2CN(CC3)C)C4=CC=C(Cl)C=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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