1. Metabolic Enzyme/Protease
    Anti-infection
  2. Cathepsin
    SARS-CoV
  3. ONO-5334

ONO-5334 

Cat. No.: HY-108044 Purity: 99.83%
Handling Instructions

ONO-5334 is a potent, selective and orally active cathepsin K inhibitor with Ki values of 0.10 nM, 0.049 nM and 0.85 nM for human, rabbit and rat cathepsin K, respectively. ONO 5334 is an effective antiviral compound against SAR-COV-2 virus activity with an EC50 value of 500 nM. ONO-5334 has the potential for the study of osteoporosis and COVID-19 disease.

For research use only. We do not sell to patients.

ONO-5334 Chemical Structure

ONO-5334 Chemical Structure

CAS No. : 868273-90-9

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5 mg USD 500 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

ONO-5334 is a potent, selective and orally active cathepsin K inhibitor with Ki values of 0.10 nM, 0.049 nM and 0.85 nM for human, rabbit and rat cathepsin K, respectively. ONO 5334 is an effective antiviral compound against SAR-COV-2 virus activity with an EC50 value of 500 nM. ONO-5334 has the potential for the study of osteoporosis and COVID-19 disease[1].

IC50 & Target

Ki: 0.10 nM (human cathepsin K)
Ki: 0.049 nM (rabbit cathepsin K)
Ki: 0.85 nM (rat cathepsin K)[1]

In Vitro

ONO-5334 has inhibitory effects on human cathepsin S, human cathepsin L, human cathepsin B, porcine calpain Ι and porcine calpain II with Ki values of 0.83 nM, 1.7 nM, 32 nM, 82 nM and 69 nM, respectively[1].
ONO-5334 (0.1-1 μM; 24 hours) suppresses human osteoclast-mediated bone resorption. It potently reduces osteoclast-mediated release of CTX from bone slices as a dose dependent manner[1].
ONO-5334 (0-10 μM; pre-treated for 16 h) inhibits antiviral activities in a discernable dose-dependent manner in Vero E6 cells by designed to capture multicycle replication, exhibiting an EC50 value of 0.5 μM[2]/

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Vero E6 cells
Concentration: 0.001 μM, 0.003 μM, 0.1 μM, 0.3 μM, 1 μM, 2.5 μM
Incubation Time: Pre-treated for 16 h and then cultured for 24 hours
Result: Inhibited SARS-COV-2 virus replication in a dose-dependent manner.
In Vivo

ONO-5334 (oral administration; 0.12-15 mg/kg; single dose) can dose-dependently reduce PTHrP-induced increase in plasma calcium with significant effect (86% reduction) at 15 mg/kg. It also reduces PTHrP-induced increase in plasma CTX level in TPTX rats by 90% at 15 mg/kg[1].
ONO-5334 (oral administration; 0.3-30 mg/kg; 7 consecutive days) at 3 mg/kg or 30 mg/kg significantly decreases CTX (a bone resorption marker) concentration. On day 7, the reduction in serum CTX concentration by ONO-5334 at 3 mg/kg and 30 mg/kg was 62% and 79%, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Monkey[1]
Dosage: 0.3 mg/kg; 3 mg/kg
Administration: Oral administration; 7 consecutive days
Result: Reduced bone resorption markers but not bone formation markers in normal monkeys.
Clinical Trial
Molecular Weight

438.58

Formula

C₂₁H₃₄N₄O₄S

CAS No.

868273-90-9

SMILES

O=C(C(N/N=C(SC[[email protected]]1C)/N1C)=O)[[email protected]](C2CCOCC2)NC(C3CCCCCC3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
References
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Keywords:

ONO-5334ONO5334ONO 5334CathepsinSARS-CoVSARS coronaviruspostmenopausalosteoporosisosteoclastPBMCsbonemineraldensityBMDSAR-COV-2COVID-19Inhibitorinhibitorinhibit

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ONO-5334
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HY-108044
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