Perfluorononanoic acid
Based on 1 Customer Validation
Perfluorononanoic acid (PFNA) is an orally active PPARα activator. Perfluorononanoic acid activates PPARα-mediated gene expression, including upregulating target genes associated with lipid metabolism and triglyceride storage. Perfluorononanoic acid exhibits certain developmental and reproductive toxicity. Perfluorononanoic acid causes hepatomegaly in pregnant mice, induces high postnatal mortality in neonatal mice, and leads to dose-dependent delays in eye-opening time and puberty onset in mouse offspring.
For research use only. We do not sell to patients.
- Purity: 99.9%
- CAS No.: 375-95-1
- Formula: C9HF17O2
- Molecular Weight:464.08
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Biological Activity
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PPARα |
Perfluorononanoic acid (6.25-400 µM; 6-72 h) reduces the viability of differentiated human HepaRG hepatocytes in a concentration- and time-dependent manner[1].
Perfluorononanoic acid (25-200 µM; 24 h) increases intracellular triglyceride levels in differentiated human HepaRG hepatocytes, with a BMC50 of 92 µM, and does not significantly alter intracellular cholesterol levels after 24 h of exposure[1].
Perfluorononanoic acid (100 µM; 24 h) alters the expression of 1024 genes in differentiated human HepaRG hepatocytes, induces pathways associated with tRNA aminoacylation, amino acid transport, and endoplasmic reticulum stress (the PERK/ATF4 signaling pathway), and simultaneously suppresses the cholesterol biosynthesis and glycolysis/gluconeogenesis pathways[1].
Perfluorononanoic acid (12.5-200 µM; 24 h) regulates gene expression in differentiated human HepaRG hepatocytes in a concentration-dependent manner, upregulating PPARα target genes and downregulating ADH4 and cholesterol biosynthesis-related genes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:differentiated human HepaRG liver cells
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Concentration:6.25, 12.5, 25, 50, 100, 200, 400 µM
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Incubation Time:6 h, 24 h, 72 h
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Result:Reduced viability obviously at 400 µM (6, 24, 72 h), 200 (24, 72 h).
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Cell Line:differentiated human HepaRG liver cells
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Concentration:12.5, 25, 50, 100, 200 µM
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Incubation Time:24 h
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Result:Caused concentration-dependent increases in expression of PPARα target genes: ANGPTL4 (100, 200 µM), PDK4 (50, 100, 200 µM), PLIN2 (50, 100, 200 µM), and PLIN4 (100 µM), with no significant effect on CPT1A.
Caused concentration-dependent decreases in expression of ADH4 (25, 50, 100, 200 µM) and cholesterogenic genes LSS (12.5, 25, 50, 100, 200 µM), FDPS (25, 50, 100, 200 µM), HMGCR (25, 50, 100, 200 µM), EBP (100, 200 µM), IDI1 (100, 200 µM), and ACAT2 (100, 200 µM).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CD-1 mice (female timed-pregnant; male and female offspring)[2]
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Dosage:1 mg/kg; 3 mg/kg; 5 mg/kg; 10 mg/kg
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Administration:oral gavage; daily; gestational day 1-17
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Result:Led to maternal weight loss and full litter resorption (10 mg/kg dose).
Induced dose‑dependent maternal and fetal hepatomegaly without affecting implantation or fetal viability (doses ≤5 mg/kg).
Caused 80% neonatal death within postnatal day 10 (5 mg/kg).
Activated PPARα, CAR, and PXR target genes in fetal and neonatal livers, and its accumulation persisted in tissues long after exposure, and surviving offspring showed delayed eye opening and puberty onset.
Chemical Information
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CAS No. 375-95-1
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Appearance Solid
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Molecular Weight 464.08
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Formula C9HF17O2
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Color White to off-white
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SMILES
C(=O)(C(C(C(C(C(C(C(C(F)(F)F)(F)F)(F)F)(F)F)(F)F)(F)F)(F)F)(F)F)O
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Synonyms
PFNA; Heptadecafluorononanoic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (215.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (107.74 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (641 KB)
- English - EN (641 KB)
- Français - FR (641 KB)
- Deutsch - DE (641 KB)
- Norwegian - NO (641 KB)
- Español - ES (641 KB)
- Swedish - SV (641 KB)
- Italian - IT (641 KB)
- Korean - KR (641 KB)
- Portuguese - PT (641 KB)
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Handling Instructions (2659 KB)
References
[1]. Louisse J, et al. Perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid (PFOS), and perfluorononanoic acid (PFNA) increase triglyceride levels and decrease cholesterogenic gene expression in human HepaRG liver cells. Arch Toxicol. 2020;94(9):3137-3155. [Content Brief]
[2]. Das KP, et al. Developmental toxicity of perfluorononanoic acid in mice. Reprod Toxicol. 2015;51:133-144. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 2.1548 mL | 10.7740 mL | 21.5480 mL | 53.8700 mL |
| 5 mM | 0.4310 mL | 2.1548 mL | 4.3096 mL | 10.7740 mL | |
| 10 mM | 0.2155 mL | 1.0774 mL | 2.1548 mL | 5.3870 mL | |
| 15 mM | 0.1437 mL | 0.7183 mL | 1.4365 mL | 3.5913 mL | |
| 20 mM | 0.1077 mL | 0.5387 mL | 1.0774 mL | 2.6935 mL | |
| 25 mM | 0.0862 mL | 0.4310 mL | 0.8619 mL | 2.1548 mL | |
| 30 mM | 0.0718 mL | 0.3591 mL | 0.7183 mL | 1.7957 mL | |
| 40 mM | 0.0539 mL | 0.2694 mL | 0.5387 mL | 1.3468 mL | |
| 50 mM | 0.0431 mL | 0.2155 mL | 0.4310 mL | 1.0774 mL | |
| 60 mM | 0.0359 mL | 0.1796 mL | 0.3591 mL | 0.8978 mL | |
| 80 mM | 0.0269 mL | 0.1347 mL | 0.2694 mL | 0.6734 mL | |
| 100 mM | 0.0215 mL | 0.1077 mL | 0.2155 mL | 0.5387 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Perfluorononanoic acid Related Classifications
HY-W203683 Related Classifications
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