Cat. No.: HY-N0408

Picroside II

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

Picroside II is an antioxidant with oral activity that can reduce the production of ROS and protect the blood-brain barrier (BBB) after CI/R injury, offering neuroprotective effects. Picroside II has antioxidant, immune-regulating, antiviral properties, and inhibits apoptosis. Picroside II alleviates the inflammatory response in sepsis by suppressing the activation of the NLRP3 inflammasome and NF-κB pathways.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 39012-20-9
Formula: C₂₃H₂₈O₁₃
Molecular Weight:512.46

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Picroside II Chemical Structure

CAS No.: 39012-20-9

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All NF-κB Isoforms :

In Vitro

Picroside II (5-20 μM, 24 h) upregulates the expression of the bcl-2 gene in primary rat liver cells^[1].
Picroside II (0-300 μM, 24 h) inhibits the growth of HepG2 cells^[2].
Picroside II (10 μM, 20 h) reduces the transcription of genes involved in fatty acid uptake and synthesis in HepG2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

Primary rat hepatocytes
Concentration:

5, 10, 20 μM
Incubation Time:

24 h
Result:

Upregulated bcl-2.

Cell Viability Assay^[2]

Cell Line:

HepG2
Concentration:

0, 3, 10, 30, 100, 300 μM
Incubation Time:

24 h
Result:

Showed the inhibition rate of cell growth was 24%.

Western Blot Analysis^[2]

Cell Line:

HepG2
Concentration:

10 μM
Incubation Time:

20 h
Result:

Increased expression of SREBP-1 protein.

RT-PCR^[2]

Cell Line:

HepG2
Concentration:

10 μM
Incubation Time:

20 h
Result:

Down-regulated the expression of FATP5 and increased the expression of SCD mRNA.

In Vivo

Picroside II (0-20 mg/kg, i.g., given in two doses) protects liver cells from damage and prevents apoptosis^[1].
Picroside II (20 mg/kg, intravenous injection, given in four doses) inhibits the activation of NLRP3 inflammasome and NF-κB pathway in mice, reducing the inflammatory response in sepsis and enhancing immune function^[3].
Picroside II (20 mg/kg, intraperitoneal injection, a single dose) protects the blood-brain barrier by inhibiting oxidative signaling pathways in a rat model of ischemia-reperfusion injury^[4].
Picroside II (5-20 mg/kg, i.g., once a day for seven days) has anti-lipid peroxidation effects and protects against mitochondrial damage in mice with liver injury^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

DGalN and LPS(HY-D1056)-induced acute liver injury in mice^[1]
Dosage:

0, 5, 10, 20 mg/kg; administered 10 min before and 4 h after D-GalN and LPS administration
Administration:

i.g.
Result:

Reduced the levels of alanine aminotransferase and aspartate aminotransferase in the serum, decreased MDA content, had a dose-dependent effect on hepatocyte apoptosis, and upregulated the expression of the bcl-2 gene.

Animal Model:

Sepsis mouse model^[3]
Dosage:

20 mg/kg; four times injected (2 h, 14 h, 26 h and 38 h after CLP)
Administration:

Intravenous injection (i.v.)
Result:

Improved survival, reduced sepsis-related infiltration and attenuated lung injury, enhanced bacterial clearance, reduced IL-6, IL-1β, and TNF-α levels, inhibited splenic lymphocyte apoptosis, reduced p-NF-κB (p65) expression, and reduced IL-1β and caspase-1 levels.

Animal Model:

Rats with cerebral ischemia-reperfusion injury^[4]
Dosage:

20 mg/kg; single dose
Administration:

Intraperitoneal injection (i.p.)
Result:

Reduced the nervous system score, neuronal injury, BBB damage, ROS content and NADPH oxidase activity, down-regulated the protein levels of Rac-1, Nox2, ROCK, MLCK and MMP-2, and up-regulated the expression of claudin-5.

Animal Model:

Mouse model of liver injury induced by CCl4, D-GalN and AP^[5]
Dosage:

5, 10, 20mg/kg; daily; 7 days
Administration:

i.g.
Result:

Reduced the high levels of ALT and AST in serum caused by CCl4, D-GalN and AP, significantly alleviated liver cell damage, reduced MDA, and increased SOD and GSH-Px content in a dose-dependent manner, inhibited AP-induced liver toxicity in mice, enhanced ATPase activity, and improved mitochondrial swelling.

Chemical Information

CAS No. 39012-20-9
Appearance Solid
Molecular Weight 512.46
Formula C₂₃H₂₈O₁₃
Color White to yellow
SMILES

O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1O[C@H]2[C@@]3([H])[C@@]([C@H](OC(C4=CC=C(O)C(OC)=C4)=O)[C@@]5([H])[C@]3(CO)O5)([H])C=CO2
Structure Classification
- Terpenoids
- Iridoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (4)

All Cell Proliferation/Viability AssayCell Migration/Invasion AssayIn Vivo Efficacy StudyWB

Sort by:

Journal Impact Factor

ACS Nano

Picroside II-Encapsulated Nanoformulations as Pyroptosis Inhibitor Alleviate Cytokine Storms and Remodel Gut Microbiota Disturbances. [Abstract]

2025 Sep 16;19(36):32569-32584. PMID: 40889346
Redox Biol

Targeting MYOF suppresses pancreatic ductal adenocarcinoma progression by inhibiting ILF3-LCN2 signaling through disrupting OTUB1-mediated deubiquitination of ILF3. [Abstract]

2025 Jul:84:103665. PMID: 40381229

Picroside II purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul:84:103665. [Abstract]

MTS proliferation assay to detect the proliferative ability of cells in Picroside II (300 μM)-treated and untreated groups.

Picroside II purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul:84:103665. [Abstract]

Transwell migration assay treated with Picroside II (300 μM).

Picroside II purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul:84:103665. [Abstract]

Images of subcutaneous xenografts from mice in Picroside II (0.4 mg/kg, i.p.)-treated and untreated groups.

Picroside II purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jul:84:103665. [Abstract]

Expression of MYOF, ILF3, OTUB1, LCN2 after Picroside II (300 μM) treatment.
Eur J Pharmacol

The therapeutic effect of Picroside II in renal ischemia-reperfusion induced acute kidney injury: An experimental study. [Abstract]

2024 Mar 15:967:176391. PMID: 38325794
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (195.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9514 mL

9.7569 mL

19.5137 mL

5 mM

0.3903 mL

1.9514 mL

3.9027 mL

10 mM

0.1951 mL

0.9757 mL

1.9514 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.88 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hua Gao, et al. Inhibitory effect of picroside II on hepatocyte apoptosis. Acta Pharmacol Sin. 2005 Jun;26(6):729-36. [Content Brief]

[2]. Hiteshi Dhami-Shah, et al. Picroside II attenuates fatty acid accumulation in HepG2 cells via modulation of fatty acid uptake and synthesis. Clin Mol Hepatol. 2018 Mar;24(1):77-87. [Content Brief]

[3]. Huang Y, et al. Picroside II protects against sepsis via suppressing inflammation in mice. Am J Transl Res. 2016 Dec 15;8(12):5519-5531. eCollection 2016. [Content Brief]

[4]. Zhai L, et al. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia-reperfusion injury. PLoS One. 2017 Apr 7;12(4):e0174414. [Content Brief]

[5]. Hua Gao, et al. Anti-lipid peroxidation and protection of liver mitochondria against injuries by picroside II. World J Gastroenterol. 2005 Jun 28;11(24):3671-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9514 mL	9.7569 mL	19.5137 mL	48.7843 mL
	5 mM	0.3903 mL	1.9514 mL	3.9027 mL	9.7569 mL
	10 mM	0.1951 mL	0.9757 mL	1.9514 mL	4.8784 mL
	15 mM	0.1301 mL	0.6505 mL	1.3009 mL	3.2523 mL
	20 mM	0.0976 mL	0.4878 mL	0.9757 mL	2.4392 mL
	25 mM	0.0781 mL	0.3903 mL	0.7805 mL	1.9514 mL
	30 mM	0.0650 mL	0.3252 mL	0.6505 mL	1.6261 mL
	40 mM	0.0488 mL	0.2439 mL	0.4878 mL	1.2196 mL
	50 mM	0.0390 mL	0.1951 mL	0.3903 mL	0.9757 mL
	60 mM	0.0325 mL	0.1626 mL	0.3252 mL	0.8131 mL
	80 mM	0.0244 mL	0.1220 mL	0.2439 mL	0.6098 mL
	100 mM	0.0195 mL	0.0976 mL	0.1951 mL	0.4878 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Picroside II

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Antiviral Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Ferroptosis Compound Library

Medicine Food Homology Compound Library

Terpenoids Library

Pyroptosis Compound Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Cancer Natural Product Library

Antidepressant Compound Library

Antiviral Traditional Chinese Medicine Active Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Inflammasomes related Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Picroside II

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Picroside II Related Classifications

Cat. No.: HY-N0408