Large-scale production of N-acetyllactosamine through bacterial coupling

  • Carbohydr Res. 1999 Mar 31;316(1-4):179-83. doi: 10.1016/s0008-6215(99)00050-6.
T Endo  1 ,  S Koizumi ,  K Tabata ,  S Kakita ,  A Ozaki
Affiliations
PMID: 10420596 DOI: 10.1016/s0008-6215(99)00050-6
Abstract

A large-scale production system of N-acetyllactosamine, a core structure of various oligosaccharides, was established by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes. Two recombinant E. coli strains over-expressed the UDP-Gal biosynthetic genes and the beta-(1-->4)-galactosyltransferase gene of Neisseria gonorrhoeae, respectively. C. ammoniagenes contributed the production of UTP from orotic acid. N-Acetyllactosamine was accumulated at 279 mM (107 g L-1) after a 38 h reaction (2.5 L in volume) starting from orotic acid, D-galactose, and 2-acetamido-2-deoxy-D-glucose.

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