Cytoprotective constituent of Hoveniae Lignum on both Hep G2 cells and rat primary hepatocytes

  • Arch Pharm Res. 2007 Jun;30(6):674-7. doi: 10.1007/BF02977626.
Ren-Bo An  1 ,  Eun-Jeon Park ,  Gil-Saeng Jeong ,  Dong-Hwan Sohn ,  Youn-Chul Kim
Affiliations
  • 1. College of Pharmacy and Phytofermentation Research Center, Wonkwang University, Iksan 570-749, Korea.
PMID: 17679542 DOI: 10.1007/BF02977626
Abstract

A phytochemical investigation of the EtOH extract of Hoveniae Lignum yielded four phenolic compounds, phloretin (1), 5-(4'-hydroxyphenyl)-gamma-valerolactone (2), (-)-epiafzelechin (3), and maesopsin (4). Compound 1 was hepatoprotective against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells with an EC50 value of 37.55 +/- 0.42 microM. Compound 1 (0.4-200 microM) also significantly reduced tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes as measured by the cellular leakage of Lactate Dehydrogenase and the level of aspartate transaminase.

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