Elucidation of triacylglycerols in cod liver oil by liquid chromatography electrospray tandem ion-trap mass spectrometry

  • Talanta. 2010 Sep 15;82(4):1261-70. doi: 10.1016/j.talanta.2010.06.055.
Ying-Xu Zeng  1 ,  Pedro Araujo ,  Zhen-Yu Du ,  Thu-Thao Nguyen ,  Livar Frøyland ,  Bjørn Grung
Affiliations
  • 1. National Institute of Nutrition and Seafood Research NIFES, PO Box 2029, Nordnes, N-5817 Bergen, Norway.
PMID: 20801327 DOI: 10.1016/j.talanta.2010.06.055
Abstract

Though liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS(2)) has been widely used in the structural elucidation of triacylglycerols (TAG) in vegetable oils, its potentiality for the identification of TAG molecules in omega-3 rich oils remains unexplored till date. Hence, this article investigates the applicability of LC-ESI-MS(2) for the structural characterization of naturally occurring TAG in cod liver oil without the TAG fractionation during the sample preparation. A computational algorithm was developed to automatically interpret the mass spectra and elucidate the TAG structures respectively. The results were compared against the Lipase benchmark method. A principal component analysis study revealed that it is possible to discriminate genuine from adulterated cod liver oil.

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