Immunomodulatory Activity of Octenyl Succinic Anhydride Modified Porang (Amorphophallus oncophyllus) Glucomannan on Mouse Macrophage-Like J774.1 Cells and Mouse Primary Peritoneal Macrophages

  • Molecules. 2017 Jul 15;22(7):1187. doi: 10.3390/molecules22071187.
Sellen Gurusmatika  1  2 ,  Kosuke Nishi  3  4 ,  Eni Harmayani  5 ,  Yudi Pranoto  6 ,  Takuya Sugahara  7  8  9
Affiliations
  • 1. Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan. [email protected].
  • 2. Faculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, Indonesia. [email protected].
  • 3. Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan. [email protected].
  • 4. Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime 790-8566, Japan. [email protected].
  • 5. Faculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, Indonesia. [email protected].
  • 6. Faculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, Indonesia. [email protected].
  • 7. Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan. [email protected].
  • 8. Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime 790-8566, Japan. [email protected].
  • 9. South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime 798-4292, Japan. [email protected].
PMID: 28714872 DOI: 10.3390/molecules22071187
Abstract

Porang is a local plant of Indonesia, which has a high content of glucomannan. In this study, porang glucomannan (PG) was esterified with octenyl succinic anhydride (OSA) to enhance emulsion properties to be widely used in food industry. OSA-modified PG (OSA-PG) enhanced the phagocytosis activity of macrophage-like J774.1 cells and mouse peritoneal macrophages. In addition, OSA-PG increased the production of IL-6 and TNF-α by enhancing their gene expression. Immunoblot analysis displayed that OSA-PG tended to activate both nuclear factor-κB and mitogen-activated protein kinase cascades. Treatment of OSA-PG with polymyxin B revealed that cytokine production induced by OSA-PG was not caused by endotoxin contamination. Our findings also indicated that OSA-PG activates macrophages through not only Toll-like Receptor (TLR) 4, but another receptor. Overall findings suggested that OSA-PG has a potential as an immunomodulatory food factor by stimulating macrophages.

Keywords
J774.1 cell; cytokine; macrophage; octenyl succinic anhydride; phagocytosis activity; porang glucomannan.
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