β-1,3-glucanase disrupts biofilm formation and increases antifungal susceptibility of Candida albicans DAY185

  • Int J Biol Macromol. 2018 Mar:108:942-946. doi: 10.1016/j.ijbiomac.2017.11.003.
Yulong Tan  1 ,  Su Ma  2 ,  Matthias Leonhard  3 ,  Doris Moser  4 ,  Berit Schneider-Stickler  3
Affiliations
  • 1. Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: [email protected].
  • 2. Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
  • 3. Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
  • 4. Department of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria.
PMID: 29104052 DOI: 10.1016/j.ijbiomac.2017.11.003
Abstract

β-1,3-glucan plays a role in Candida biofilm formation and survival of biofilm-forming Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1→3)-glucose of Candida albicans biofilms. Biofilm was dispersed by 55.96%. β-1,3-glucanase had no effect on Candida planktonic growth as well as adhesion. β-1,3-glucanase markedly enhanced the Antifungal susceptibility of fluconazole and amphotericin B. The examination using confocal laser scanning microscopy and scanning electron microscope confirmed the antibiofilm activity of β-1,3-glucanase. Our findings demonstrate that β-1,3-glucanase may be useful as an antibiofilm agent.

Keywords
Antibiofilm; Antifungal; Enzyme.
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