Iridoids from the Roots of Patrinia scabra and Their Inhibitory Potential on LPS-Induced Nitric Oxide Production

  • J Nat Prod. 2018 Jun 22;81(6):1468-1473. doi: 10.1021/acs.jnatprod.8b00229.
Da Hye Lee  1 ,  Ji-Sun Shin  1 ,  Shin-Young Kang  1 ,  Seung-Bin Lee  1 ,  Jin Su Lee  1 ,  Seung Mok Ryu  2 ,  Kyung Tae Lee  1 ,  Dongho Lee  2 ,  Dae Sik Jang  1
Affiliations
  • 1. Department of Life and Nanopharmaceutical Sciences, Graduate School , Kyung Hee University , Seoul 136-713 , Korea.
  • 2. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology , Korea University , Seoul 130-701 , Republic of Korea.
PMID: 29799195 DOI: 10.1021/acs.jnatprod.8b00229
Abstract

An activity-guided fractionation procedure of the 70% aqueous EtOH extract from the roots of Patrinia scabra led to the isolation and characterization of five new Iridoids, patriscabrins A-E (1-5), along with 13 known compounds. The structures of 1-5 were determined by interpretation of spectroscopic data, particularly by 1D and 2D NMR, ECD, and VCD studies. Thereafter, isolates were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. Of these, the new Iridoids 2 and 5 and the known lignan patrineolignan B (6) exhibited IC50 values of 14.7 to 17.8 μM.