Loliolide Presents Antiapoptosis and Antiscratching Effects in Human Keratinocytes

  • Int J Mol Sci. 2019 Feb 2;20(3):651. doi: 10.3390/ijms20030651.
Sang Hee Park  1 ,  Dong Sam Kim  2 ,  Sunggyu Kim  3  4 ,  Laura Rojas Lorz  5 ,  Eunju Choi  6 ,  Hye Yeon Lim  7 ,  Mohammad Amjad Hossain  8 ,  SeokGu Jang  9 ,  Young Im Choi  10 ,  Kyung Ja Park  11 ,  Keejung Yoon  12 ,  Jong-Hoon Kim  13 ,  Jae Youl Cho  14  15
Affiliations
  • 1. Department of Biocosmetics, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 2. Samcheok Prasiola Japonica Research Center, Samcheok City Hall, Samcheok 25914, Korea. [email protected].
  • 3. Department of Biocosmetics, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 4. Research and Business Foundation, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 5. Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 6. Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 7. Department of Biocosmetics, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 8. Department of Veterinary Physiology, College of Medicine, Chonbuk National University, Iksan 54596, Korea. [email protected].
  • 9. Samcheok Prasiola Japonica Research Center, Samcheok City Hall, Samcheok 25914, Korea. [email protected].
  • 10. Samcheok Prasiola Japonica Research Center, Samcheok City Hall, Samcheok 25914, Korea. [email protected].
  • 11. Samcheok Prasiola Japonica Research Center, Samcheok City Hall, Samcheok 25914, Korea. [email protected].
  • 12. Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 13. Department of Veterinary Physiology, College of Medicine, Chonbuk National University, Iksan 54596, Korea. [email protected].
  • 14. Department of Biocosmetics, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
  • 15. Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea. [email protected].
PMID: 30717391 DOI: 10.3390/ijms20030651
Abstract

Loliolide is a monoterpenoid hydroxylactone present in freshwater algae that has anti-inflammatory and antiaging activity; however, its effects on ultraviolet-damaged skin have yet to be elucidated. This study investigated the antiapoptosis and wound-healing effects of loliolide using HaCaT cells (a human keratinocyte cell line). Loliolide inhibited the expression of Reactive Oxygen Species (ROS) induced by ultraviolet radiation as well as wrinkle formation-related matrix metalloproteinase genes and increased the expression of the damage repair-related gene SIRT1. The Apoptosis signaling pathway was confirmed by Western blot analysis, which showed that loliolide was able to reduce the expression of caspases 3, 8, and 9, which are related to ROS-induced Apoptosis. In addition, Western blotting, reverse-transcription polymerase chain reaction (PCR), and Real-Time PCR analyses showed that loliolide enhanced the expression of the epidermal growth factor receptor signaling pathway (PI3K, Akt) and migration factors, such as K6, K16, and K17; keratinocyte growth factor; and inflammatory cytokines, such as interleukin (IL)-1, IL-17, and IL-22 expressed during the cellular scratching process, suggesting a putative wound-healing ability. Because of the antiapoptosis and antiscratching effects on skin of both loliolide and loliolide-rich Prasiola japonica ethanol extract, we consider the former to be an important compound used in the cosmeceutical industry.

Keywords
antiapoptosis; antioxidant activity; antiscratching effect; antiwrinkling activity.
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