Isolation of angiotensin-converting enzyme inhibitor from tuna muscle

  • Biochem Biophys Res Commun. 1988 Aug 30;155(1):332-7. doi: 10.1016/s0006-291x(88)81089-1.
Y Kohama  1 ,  S Matsumoto ,  H Oka ,  T Teramoto ,  M Okabe ,  T Mimura
Affiliations
  • 1. Faculty of Pharmaceutical Sciences, Osaka University, Japan.
PMID: 3415688 DOI: 10.1016/s0006-291x(88)81089-1
Abstract

A novel inhibitor of angiotensin-converting enzyme (ACE) has been discovered and isolated in a pure form from acid extract of tuna muscle by successive column chromatographies and HPLC. The final preparation showed IC50 values of 1 microM and 2 microM for ACEs from bovine and rabbit lungs, respectively. The amino acid sequence of the inhibitor has been established as Pro-Thr-His-Ile-Lys-Trp-Gly-Asp by the Edman procedure and Carboxypeptidase digestion.

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