Mitochondrial Membrane Potential Assay

  • Methods Mol Biol. 2022;2474:11-19. doi: 10.1007/978-1-0716-2213-1_2.
Srilatha Sakamuru  1 ,  Jinghua Zhao  1 ,  Matias S Attene-Ramos  2 ,  Menghang Xia  3
Affiliations
  • 1. National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA.
  • 2. Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
  • 3. National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA. [email protected].
PMID: 35294751 DOI: 10.1007/978-1-0716-2213-1_2
Abstract

Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2, HepaRG, and AC16 cells. A homogenous cell-based MMP assay has been optimized and performed in a 1536-well plate format, which can be used to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.

Keywords
1536-well plate; Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone (FCCP); Mitochondrial membrane potential (MMP); Mitochondrial membrane potential indicator (m-MPI); Mitochondrial toxicity.