Tumor Suppressor miR-613 Alleviates Non-Small Cell Lung Cancer Cell via Repressing M2 Macrophage Polarization

  • J Oncol. 2023 Feb 16:2023:2311231. doi: 10.1155/2023/2311231.
Mingjun Yang  1 ,  Wen Zhou  1 ,  Mingming Xu  1 ,  Xiao Han  1 ,  Yanyan Shi  1 ,  Min Shi  1 ,  Zhipeng Wang  2
Affiliations
  • 1. Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, No. 20, Xisi Road, Nantong, Jiangsu 226001, China.
  • 2. Department of Thoracic Surgery, Haimen People's Hospital, No. 1201, Beijing Road, Nantong, Jiangsu 226001, China.
PMID: 36844868 DOI: 10.1155/2023/2311231
Abstract

Background: Non-small cell lung Cancer (NSCLC) is a crucial crux of cancer-related death, and M2 macrophage polarization facilitates NSCLC development. MicroRNA-613 (miR-613) is a tumor suppressor. This research aimed to clarify the miR-613 function in NSCLC and its impact on M2 macrophage polarization. Methods. miR-613 expressions in NSCLC tissues and cells were evaluated using quantitative Real-Time PCR. For miR-613 function in NSCLC, cell proliferation analysis, cell counting kit-8, flow cytometry, western blot, transwell, and wound-healing were conducted. Meanwhile, the miR-613 impact on M2 macrophage polarization was assessed by the NSCLC models. Results. miR-613 was lessened in NSCLC cells and tissues. It was corroborated that miR-613 overexpression retrained NSCLC cell proliferation, invasion, and migration but facilitated cell Apoptosis. Moreover, miR-613 overexpression restrained NSCLC development by repressing M2 macrophage polarization.

Conclusion: Tumor suppressor miR-613 ameliorated NSCLC by restraining M2 macrophage polarization.

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