Quaternary ammonium carboxymethyl chitosan composite hydrogel with efficient antibacterial and antioxidant properties for promoting wound healing

  • Int J Biol Macromol. 2024 May;268(Pt 2):131871. doi: 10.1016/j.ijbiomac.2024.131871.
Yahao Ma  1 ,  Xujie Zhou  1 ,  Zhendong Mo  1 ,  Qing Zhou  1 ,  Bingyu Hui  1 ,  Zhuangzhuang Cai  1 ,  Xiaoying Wang  2 ,  Hang Li  3 ,  Shunqing Tang  4
Affiliations
  • 1. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
  • 2. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: [email protected].
  • 3. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: [email protected].
  • 4. Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address: [email protected].
PMID: 38677691 DOI: 10.1016/j.ijbiomac.2024.131871
Abstract

Multifunctional hydrogels have been developed to meet the various requirements of wound healing. Herein, an innovative hydrogel (QCMC-HA-PEG) was formed through the Schiff base reaction, composed of quaternary ammonium-modified carboxymethyl chitosan (QCMC), hyaluronic acid (HA), and 8-arms Polyethylene Glycol aldehyde (8-ARM-PEG-CHO). The resulting hydrogels exhibited good mechanical and adhesive properties with improved Antibacterial efficacy against both Gram-positive and Gram-negative bacteria compared to CMC hydrogels. QCMC-HA-PEG hydrogels demonstrated remarkable adhesive ability in lap-shear test. Furthermore, the incorporation of MnO2 nanosheets into the hydrogel significantly enhanced its reactive oxygen species (ROS) scavenging and oxygen generation capabilities. Finally, experimental results from a full-thickness skin wound model revealed that the QCMC-HA-PEG@MnO2 hydrogel promoted skin epithelization, Collagen deposition, and inflammatory regulation significantly accelerated the wound healing process. Therefore, QCMC-HA-PEG@MnO2 hydrogel could be a promising wound dressing to promote wound healing.

Keywords
Adhesive; Antibacterial; Antioxidant; Hydrogel; Wound healing.
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