Labelling of a live obligate anaerobe using fluorescent D-amino acids

  • Anaerobe. 2025 Feb:91:102939. doi: 10.1016/j.anaerobe.2025.102939.
Benjamin Lewis  1 ,  Adenrele Oludiran  1 ,  Ann Progulske-Fox  2 ,  William Dunn  3
Affiliations
  • 1. Center for Molecular Microbiology, University of Florida, Gainesville, FL, 32610, USA.
  • 2. Center for Molecular Microbiology, University of Florida, Gainesville, FL, 32610, USA. Electronic address: [email protected].
  • 3. Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL, 32610, USA.
PMID: 39814196 DOI: 10.1016/j.anaerobe.2025.102939
Abstract

The probing of live bacteria via the incorporation of fluorescent D-amino acids (FDAAs) during peptidoglycan synthesis has been shown to be practical for visualizing both gram-positive and gram-negative Bacterial species. This study demonstrates the reliability and applications of FDAA labelling for the fluorescent imaging of an obligate anaerobe.

Keywords
Anaerobic; Fluorescence; Microscopy; P. gingivalis; Peptidoglycan; Porphyromonas gingivalis.
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