UPLC-ESI-TOF-MS Profiling of Metabolome Alterations in Barley (Hordeum vulgare L.) Leaves Induced by Bipolaris sorokiniana

  • J Agric Food Chem. 2025 Oct 1;73(39):24662-24687. doi: 10.1021/acs.jafc.5c05419.
Lisa Kurzweil  1 ,  Timo D Stark  2 ,  Karina Hille  2 ,  Felix Hoheneder  3 ,  Jana Mrtva  2 ,  Johann Hausladen  4 ,  Miriam Lenk  5 ,  Mohammed Saddik Motawia  6 ,  Nicole Strittmatter  7 ,  A Corina Vlot  5  8 ,  Klaus Pillen  9 ,  Mette So̷rensen  6  10 ,  Birger L Mo̷ller  6 ,  Ralph Hückelhoven  3 ,  Corinna Dawid  1  2  11
Affiliations
  • 1. Professorship for Functional Phytometabolomics, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
  • 2. Chair of Food Chemistry and Molecular Sensory Science, TUM School of Life Sciences, Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
  • 3. Chair of Phytopathology, TUM School of Life Sciences, Technical University of Munich, Emil-Ramann-Str. 2, 85354 Freising, Germany.
  • 4. Plant Technology Center, TUM School of Life Sciences, Technical University of Munich, Dürnast 9, 85354 Freising, Germany.
  • 5. Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
  • 6. Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiskberg C, Copenhagen Denmark.
  • 7. Professorship for Analytical Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748 Garching, Germany.
  • 8. Chair of Crop Plant Genetics, Faculty of Life Sciences: Food, Nutrition and Health, University of Bayreuth, Fritz-Hornschuch-Straße 13, 95326 Kulmbach, Germany.
  • 9. Chair of Plant Breeding, Martin-Luther-University Halle-Wittenberg, Betty-Heimann-Str. 3, 06120 Halle (Saale), Germany.
  • 10. Novo Nordisk Pharmatech, Ko̷benhavnsvej 216, 4600 Ko̷ge, Copenhagen Denmark.
  • 11. Leibniz Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Str. 34, 85354 Freising, Germany.
PMID: 40963221 DOI: 10.1021/acs.jafc.5c05419
Abstract

Spot blotch of barley (Hordeum vulgare L.), caused by Bipolaris sorokiniana, is responsible for major losses in crop yield. Breeding-resistant barley varieties have proven to be an effective countermeasure for protecting agricultural production. Plants react to pathogen attacks by up-regulating secondary metabolites. Marker compounds for a B. sorokiniana Infection are examined by untargeted UPLC-TOF-MS metabolomics and lipidomics techniques. Through the analysis of nine quantitatively resistant and susceptible barley genotypes, derived from the nested association mapping population HEB-25, followed by structure identification experiments and spore germination assays, 57 metabolites are identified. In addition to previously known metabolites, the unknown compounds 5-carboxydidehydroblumenol C-9-O-ß-d-glucoside (46) and grasshopper ketone 3-sulfate (47) were elucidated. 5-Carboxyblumenol C-9-O-ß-d-glucoside (45) was described for the first time in barley leaves. Pheophytin derivatives, oxylipins, linolenate-conjugated lipids, and flavone glycosides were described for the first time in connection with infections by phytopathogenic fungi or resistance in barley.

Keywords
Bipolaris sorokiniana; MS imaging; barley; lipids; metabolomics; untargeted LC−MS.
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