cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme

  • Biosci Biotechnol Biochem. 1998 May;62(5):1037-40. doi: 10.1271/bbb.62.1037.
K Yanagisawa  1 ,  Y Sakakibara ,  M Suiko ,  Y Takami ,  T Nakayama ,  H Nakajima ,  K Takayanagi ,  Y Natori ,  M C Liu
Affiliations
  • 1. Department of Biochemistry, University of Texas Health Center, Tyler 75710, USA.
PMID: 9648242 DOI: 10.1271/bbb.62.1037
Abstract

A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities.