2. Recombinant Proteins
  3. Ubiquitin Related Proteins
  4. DCAF16 Protein, Human (Sf9)

DCAF16 Protein, Human (Sf9)

Cat. No.: HY-P701517
Handling Instructions

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16 Protein, Human (Sf9) is the recombinant human-derived DCAF16 protein, expressed by Sf9 insect cells , with tag free. The total length of DCAF16 Protein, Human (Sf9) is 215 a.a., .

For research use only. We do not sell to patients.

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Description

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16 Protein, Human (Sf9) is the recombinant human-derived DCAF16 protein, expressed by Sf9 insect cells , with tag free. The total length of DCAF16 Protein, Human (Sf9) is 215 a.a., .
Background

DCAF16 protein serves as a crucial substrate recognition component within the CUL4-DDB1 E3 ubiquitin-protein ligase complex, playing a central role in the ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins. This function highlights its involvement in protein modification processes, specifically in the regulation of protein ubiquitination.
Species

Human
Source

Sf9 insect cells
Tag

Tag Free
Accession

Q9NXF7 (G2-L216)
Gene ID

54876
Molecular Construction
N-term
DCAF16 (G2-L216)
Accession # Q9NXF7
C-term
Synonyms
DCAF16; DDB1- and CUL4-associated factor 16
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DCAF16 Protein, Human (Sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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