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amylase substrate

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By Targets:	Amylases (3) Bacterial (2) Biochemical Assay Reagents (4) Endogenous Metabolite (3) Glycosidase (2) Integrin (1) NF-κB (1) P-glycoprotein (1) Reference Standards (1) TNF Receptor (2)
By Research Areas:	Cardiovascular Disease (1) Inflammation/Immunology (2) Metabolic Disease (2)

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Biochemical Assay Reagents

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Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-N2464

Maltotetraose 1 Publications Verification Amylotetraose; Fujioligo 450; α-1,4-Tetraglucose	TNF Receptor Endogenous Metabolite Bacterial Integrin NF-κB	Cardiovascular Disease Inflammation/Immunology
Maltotetraose can serve as a substrate for enzyme-linked assays to measure amylase activity in biological fluids. Maltotetraose has oral active, and reduces TNF-α-induced inflammatory responses by inhibiting NF-κB activity and decreasing ICAM-1 expression. Maltotetraose also inhibits PDGF-induced vascular smooth muscle cell migration and neovascularization. Additionally, Maltotetraose derivatives can function as probes for detecting bacterial infections by targeting the maltodextrin transporter. With good long-term safety, Maltotetraose holds promise for research in atherosclerosis-related diseases .

HY-N1495

Maltopentaose Maltopentose	Amylases	Others
Maltopentaose is the shortest chain oligosaccharide. Maltopentaose is a substrate for *α-amylases. Maltopentaose can be classified as maltodextrin and is also used in a study to investigate glycation and phosphorylation of α-lactalbumin. Maltopentaose is used to study the inhibition kinetics of human pancreatic α-amylase* by dehydrodieugenol B .

HY-153102

G3-CNP	Biochemical Assay Reagents	Others
G3-CNP is an α-amylase substrate. The absorbance of G3-CNP cleavage product 2-chloro-4-nitrophenol is measured at 405 nm, which can be used to detect enzyme activity .

HY-112835

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside pNP-G7	Amylases Glycosidase	Inflammation/Immunology
Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside (EPS; pNP-G7) serves as a substrate for α-amylase. In the presence of an auxiliary enzyme such as α-glucosidase (α-glucosidase), Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is degraded by amylase (Amylase) to release a chromophore, enabling the measurement of amylase activity. Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is applicable for the diagnosis of pancreatitis ^[2].

HY-153101

Gal-G2-CNP	Biochemical Assay Reagents	Others
Gal-G2-CNP is a galactopyranosyl maltoside. Gal-G2-CNP is an amylase substrate specific to salivary amylase, which produces a yellow hydrolyzate upon decomposition. Gal-G2-CNP can serve as a matrix for the assays of novel amylases and pancreatic amylases .

HY-N8326

Maltononaose	Others	Others
Maltononaose is a linear oligosaccharide consisting of 9 glucose units linked by alpha-1, 4-glucoside bonds. Maltononaose is used as a substrate to study the subsites affinity of glucoamylase. Maltononaose can be used to determine the activity of amylase and to optimize the process of starch hydrolysis .

HY-137228

4-Nitrophenylmaltohexaoside	Biochemical Assay Reagents	Others
4-Nitrophenylmaltohexaoside is a chromogenic substrate targeting α-amylase.

HY-137785

2-Chloro-4-nitrophenyl-β-D-maltotrioside	Biochemical Assay Reagents	Others
2-Chloro-4-nitrophenyl-β-D-maltotrioside is a chromogenic substrate used in bioluminescent and fluorescent assays to detect α-amylase.

HY-N2464R

Maltotetraose (Standard) Amylotetraose (Standard); Fujioligo 450 (Standard); α-1,4-Tetraglucose (Standard)	TNF Receptor Reference Standards Endogenous Metabolite Bacterial	Metabolic Disease
Maltotetraose (Standard) is the analytical standard of Maltotetraose. This product is intended for research and analytical applications. Maltotetraose can be used as a substrate for the enzyme-coupled determination of amylase activity in biological fluids.

HY-162373

α-Amylase/α-Glucosidase-IN-10	Amylases Glycosidase P-glycoprotein	Metabolic Disease
α-Amylase/α-Glucosidase-IN-10 (compound 5d) is an α-amylase and α-glucosidase inhibitor (IC₅₀: 30.39 μM and 65.1 μM) with potential diabetes inhibitory effects. α-Amylase/α-Glucosidase-IN-10 exhibits high gastrointestinal (GI) absorption in ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) prediction. While α-Amylase/α-Glucosidase-IN-10 acts as a substrate for P-gp and does not cross the blood-brain barrier (BBB), there may be a risk of central nervous system side effects .

HY-E70325

Maltogenic amylase	Endogenous Metabolite	Others
Maltogenic amylase is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on enhancing enzyme reaction kinetics, substrate selectivity, and activity under harsh conditions such as low or high pH. By introducing stimulus responsiveness to these enzyme modifications, dynamic control of activity is also possible .

G3-CNP	Biochemical Assay Reagents
G3-CNP is an α-amylase substrate. The absorbance of G3-CNP cleavage product 2-chloro-4-nitrophenol is measured at 405 nm, which can be used to detect enzyme activity .

Gal-G2-CNP	Biochemical Assay Reagents
Gal-G2-CNP is a galactopyranosyl maltoside. Gal-G2-CNP is an amylase substrate specific to salivary amylase, which produces a yellow hydrolyzate upon decomposition. Gal-G2-CNP can serve as a matrix for the assays of novel amylases and pancreatic amylases .

4-Nitrophenylmaltohexaoside	Biochemical Assay Reagents
4-Nitrophenylmaltohexaoside is a chromogenic substrate targeting α-amylase.

2-Chloro-4-nitrophenyl-β-D-maltotrioside	Biochemical Assay Reagents
2-Chloro-4-nitrophenyl-β-D-maltotrioside is a chromogenic substrate used in bioluminescent and fluorescent assays to detect α-amylase.

Maltotetraose 1 Publications Verification Amylotetraose; Fujioligo 450; α-1,4-Tetraglucose	Human Gut Microbiota Metabolites Polysaccharides Microorganisms Classification of Application Fields Gramineae Hordeum vulgare L. Metabolic Disease Plants Endogenous metabolite Disease Research Fields Saccharides Source Classification	TNF Receptor Endogenous Metabolite Bacterial Integrin NF-κB
Maltotetraose can serve as a substrate for enzyme-linked assays to measure amylase activity in biological fluids. Maltotetraose has oral active, and reduces TNF-α-induced inflammatory responses by inhibiting NF-κB activity and decreasing ICAM-1 expression. Maltotetraose also inhibits PDGF-induced vascular smooth muscle cell migration and neovascularization. Additionally, Maltotetraose derivatives can function as probes for detecting bacterial infections by targeting the maltodextrin transporter. With good long-term safety, Maltotetraose holds promise for research in atherosclerosis-related diseases .

Maltopentaose Maltopentose	Human Gut Microbiota Metabolites Polysaccharides Microorganisms Classification of Application Fields Gramineae Hordeum vulgare L. Plants Endogenous metabolite Inflammation/Immunology Disease Research Fields Saccharides Source Classification	Amylases
Maltopentaose is the shortest chain oligosaccharide. Maltopentaose is a substrate for *α-amylases. Maltopentaose can be classified as maltodextrin and is also used in a study to investigate glycation and phosphorylation of α-lactalbumin. Maltopentaose is used to study the inhibition kinetics of human pancreatic α-amylase* by dehydrodieugenol B .

Maltononaose	Structural Classification Polysaccharides Microorganisms Saccharides Source Classification	Others
Maltononaose is a linear oligosaccharide consisting of 9 glucose units linked by alpha-1, 4-glucoside bonds. Maltononaose is used as a substrate to study the subsites affinity of glucoamylase. Maltononaose can be used to determine the activity of amylase and to optimize the process of starch hydrolysis .

Maltotetraose (Standard) Amylotetraose (Standard); Fujioligo 450 (Standard); α-1,4-Tetraglucose (Standard)	Structural Classification Human Gut Microbiota Metabolites Polysaccharides Microorganisms Gramineae Hordeum vulgare L. Plants Endogenous metabolite Saccharides Source Classification	TNF Receptor Reference Standards Endogenous Metabolite Bacterial
Maltotetraose (Standard) is the analytical standard of Maltotetraose. This product is intended for research and analytical applications. Maltotetraose can be used as a substrate for the enzyme-coupled determination of amylase activity in biological fluids.

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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amylase substrate

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Biochemical Assay Reagents

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