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  3. G3-CNP

G3-CNP is an α-amylase substrate. The absorbance of G3-CNP cleavage product 2-chloro-4-nitrophenol is measured at 405 nm, which can be used to detect enzyme activity.

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G3-CNP Chemical Structure

G3-CNP Chemical Structure

CAS No. : 118291-90-0

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Based on 1 publication(s) in Google Scholar

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Description

G3-CNP is an α-amylase substrate. The absorbance of G3-CNP cleavage product 2-chloro-4-nitrophenol is measured at 405 nm, which can be used to detect enzyme activity[1].

In Vitro

G3-CNP (50 µL; 10 min) is lysed by α-amylase at 37°C to produce 2-chloro-4-nitrophenol[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

659.98

Formula

C24H34ClNO18

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H](O[C@@H]([C@@H]([C@H]1O)O)OC2=C(C=C(C=C2)[N+]([O-])=O)Cl)[C@H]1O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O[C@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)CO)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (189.40 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5152 mL 7.5760 mL 15.1520 mL
5 mM 0.3030 mL 1.5152 mL 3.0304 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.5152 mL 7.5760 mL 15.1520 mL 37.8799 mL
5 mM 0.3030 mL 1.5152 mL 3.0304 mL 7.5760 mL
10 mM 0.1515 mL 0.7576 mL 1.5152 mL 3.7880 mL
15 mM 0.1010 mL 0.5051 mL 1.0101 mL 2.5253 mL
20 mM 0.0758 mL 0.3788 mL 0.7576 mL 1.8940 mL
25 mM 0.0606 mL 0.3030 mL 0.6061 mL 1.5152 mL
30 mM 0.0505 mL 0.2525 mL 0.5051 mL 1.2627 mL
40 mM 0.0379 mL 0.1894 mL 0.3788 mL 0.9470 mL
50 mM 0.0303 mL 0.1515 mL 0.3030 mL 0.7576 mL
60 mM 0.0253 mL 0.1263 mL 0.2525 mL 0.6313 mL
80 mM 0.0189 mL 0.0947 mL 0.1894 mL 0.4735 mL
100 mM 0.0152 mL 0.0758 mL 0.1515 mL 0.3788 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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G3-CNP
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HY-153102
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