Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside
Based on 1 Customer Validation
Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside (EPS; pNP-G7) serves as a substrate for α-amylase. In the presence of an auxiliary enzyme such as α-glucosidase (α-glucosidase), Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is degraded by amylase (Amylase) to release a chromophore, enabling the measurement of amylase activity. Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is applicable for the diagnosis of pancreatitis[2].
For research use only. We do not sell to patients.
- Purity : 98.03%
- CAS No.: 96597-16-9
- Formula: C50H77NO38
- Molecular Weight:1300.13
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
α‑glucosidase |
In Vitro
Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs).
Assay conditions and protocol for determining the catalytic concentration of α-amylase (37 ℃)[1]:
1. Preparation of working solution:
1) Prepare a 21.7 mmol/L Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside solution in 52 mmol/L HEPES, pH 7.15, with a volume of 100 μL.
2. Experimental procedures:
1) Dissolve appropriate amounts of Sodium chloride and Calcium chloride in 52 mmol/L HEPES, pH 7.15, and mix thoroughly.
2) Add the test sample and Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside working solution to achieve final concentrations of 3.5 mmol/L for Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside, 7.1 kU/L for α-Glucosidase, 70 mmol/L for Sodium chloride, 1.0 mmol/L for Calcium chloride, and 50 mmol/L for HEPES.
3) Mix thoroughly, wait 120 seconds for the lag phase to end, and record the absorbance changes at 405 nm over 180 seconds. Calculate DA/Dt, the average absorbance change per minute.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 96597-16-9
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Appearance Solid
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Molecular Weight 1300.13
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Formula C50H77NO38
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O[C@@H]2[C@@H](CO)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](CO)O[C@H](O[C@@H]5[C@@H](CO)O[C@H](O[C@@H]6[C@@H](CO)O[C@H](O[C@@H]7[C@@H](CO)O[C@H](OC8=CC=C([N+]([O-])=O)C=C8)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)O[C@H]9[C@@H](OC(C)OC9)[C@@H]1O
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Synonyms
pNP-G7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (76.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.7692 mL | 3.8458 mL | 7.6915 mL | 19.2288 mL |
| 5 mM | 0.1538 mL | 0.7692 mL | 1.5383 mL | 3.8458 mL | |
| 10 mM | 0.0769 mL | 0.3846 mL | 0.7692 mL | 1.9229 mL | |
| 15 mM | 0.0513 mL | 0.2564 mL | 0.5128 mL | 1.2819 mL | |
| 20 mM | 0.0385 mL | 0.1923 mL | 0.3846 mL | 0.9614 mL | |
| 25 mM | 0.0308 mL | 0.1538 mL | 0.3077 mL | 0.7692 mL | |
| 30 mM | 0.0256 mL | 0.1282 mL | 0.2564 mL | 0.6410 mL | |
| 40 mM | 0.0192 mL | 0.0961 mL | 0.1923 mL | 0.4807 mL | |
| 50 mM | 0.0154 mL | 0.0769 mL | 0.1538 mL | 0.3846 mL | |
| 60 mM | 0.0128 mL | 0.0641 mL | 0.1282 mL | 0.3205 mL |
Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside Related Classifications
HY-112835 Related Classifications
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