2. Metabolic Enzyme/Protease
  3. Amylases Glycosidase
  4. Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside  (Synonyms: pNP-G7)

Cat. No.: HY-112835 Purity: 99.42%
COA
SDS
Handling Instructions Technical Support

Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside (EPS; pNP-G7) serves as a substrate for α-amylase. In the presence of an auxiliary enzyme such as α-glucosidase (α-glucosidase), Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is degraded by amylase (Amylase) to release a chromophore, enabling the measurement of amylase activity. Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is applicable for the diagnosis of pancreatitis[2].

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside 화학구조

CAS No. : 96597-16-9

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside (EPS; pNP-G7) serves as a substrate for α-amylase. In the presence of an auxiliary enzyme such as α-glucosidase (α-glucosidase), Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is degraded by amylase (Amylase) to release a chromophore, enabling the measurement of amylase activity. Ethylidene-4-nitrophenyl-α-D-Maltoheptaoside is applicable for the diagnosis of pancreatitis[1][2].

IC50 & Target[1]

α‑glucosidase

 

In Vitro

Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs).
Assay conditions and protocol for determining the catalytic concentration of α-amylase (37 ℃)[1]:
1. Preparation of working solution:
1) Prepare a 21.7 mmol/L Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside solution in 52 mmol/L HEPES, pH 7.15, with a volume of 100 μL.
2. Experimental procedures:
1) Dissolve appropriate amounts of Sodium chloride and Calcium chloride in 52 mmol/L HEPES, pH 7.15, and mix thoroughly.
2) Add the test sample and Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside working solution to achieve final concentrations of 3.5 mmol/L for Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside, 7.1 kU/L for α-Glucosidase, 70 mmol/L for Sodium chloride, 1.0 mmol/L for Calcium chloride, and 50 mmol/L for HEPES.
3) Mix thoroughly, wait 120 seconds for the lag phase to end, and record the absorbance changes at 405 nm over 180 seconds. Calculate DA/Dt, the average absorbance change per minute.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside Related Antibodies
분자량

1300.13

화학식

C50H77NO38
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[C@@H]2[C@@H](CO)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](CO)O[C@H](O[C@@H]5[C@@H](CO)O[C@H](O[C@@H]6[C@@H](CO)O[C@H](O[C@@H]7[C@@H](CO)O[C@H](OC8=CC=C([N+]([O-])=O)C=C8)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)O[C@H]9[C@@H](OC(C)OC9)[C@@H]1O
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

용액&용해도
In Vitro: 

DMSO : 100 mg/mL (76.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7692 mL 3.8458 mL 7.6915 mL
5 mM 0.1538 mL 0.7692 mL 1.5383 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (1.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (1.92 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서

Purity: 99.42%

SDS (393 KB)

COA (257 KB) RP-HPLC (246 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7692 mL 3.8458 mL 7.6915 mL 19.2288 mL
5 mM 0.1538 mL 0.7692 mL 1.5383 mL 3.8458 mL
10 mM 0.0769 mL 0.3846 mL 0.7692 mL 1.9229 mL
15 mM 0.0513 mL 0.2564 mL 0.5128 mL 1.2819 mL
20 mM 0.0385 mL 0.1923 mL 0.3846 mL 0.9614 mL
25 mM 0.0308 mL 0.1538 mL 0.3077 mL 0.7692 mL
30 mM 0.0256 mL 0.1282 mL 0.2564 mL 0.6410 mL
40 mM 0.0192 mL 0.0961 mL 0.1923 mL 0.4807 mL
50 mM 0.0154 mL 0.0769 mL 0.1538 mL 0.3846 mL
60 mM 0.0128 mL 0.0641 mL 0.1282 mL 0.3205 mL
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Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside96597-16-9pNP-G7AmylasesGlycosidaseacute pancreatitisα-amylasehuman pancreatic α-amylasemacroamylasemia4-nitrophenolbacterial α-glucosidaseglucosesalivary α-amylaseserum macroamylasesInhibitorinhibitorinhibit

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상품명:
Ethylidene-4-nitrophenyl-a-D-Maltoheptaoside
Cat. No.:
HY-112835
수량:
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