2. Metabolic Enzyme/Protease MAPK/ERK Pathway NF-κB Anti-infection
  3. Tyrosinase p38 MAPK NF-κB Bacterial
  4. Velutin

Velutin is a flavonoid. Velutin can be extracted from mistletoe. Velutin inhibits mushroom Tyrosinase activity with an IC50 of 910.1 μM. Velutin inhibits p38 phosphorylation, the NF-κB pathway and the MAPK pathway. Velutin prevents articular cartilage degeneration and subchondral bone loss. Velutin slows down the progression of intervertebral disc degeneration. Velutin exhibits inhibitory effects on melanogenesis, skin whitening, anti-inflammatory, anti-allergic, anti-oxidant and antibacterial activities. Velutin can be used in studies related to pigmented diseases, osteoarthritis and intervertebral disc degeneration.

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Velutin

Velutin Chemical Structure

CAS No. : 25739-41-7

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Based on 1 publication(s) in Google Scholar

Other Forms of Velutin:

Velutin Related Antibodies

Top Publications Citing Use of Products

View All p38 MAPK Isoform Specific Products:

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Velutin is a flavonoid. Velutin can be extracted from mistletoe. Velutin inhibits mushroom Tyrosinase activity with an IC50 of 910.1 μM. Velutin inhibits p38 phosphorylation, the NF-κB pathway and the MAPK pathway. Velutin prevents articular cartilage degeneration and subchondral bone loss. Velutin slows down the progression of intervertebral disc degeneration. Velutin exhibits inhibitory effects on melanogenesis, skin whitening, anti-inflammatory, anti-allergic, anti-oxidant and antibacterial activities. Velutin can be used in studies related to pigmented diseases, osteoarthritis and intervertebral disc degeneration[1][2][3].

Cellular Effect
Cell Line Type Value Description References
A2780 IC50
> 20 μg/mL
Compound: 4',5-dihydroxy-3',7-dimethoxyflavone
Cytotoxicity against human A2780 cells
Cytotoxicity against human A2780 cells
[PMID: 15217279]
KB IC50
1.5 μg/mL
Compound: 4, Velutin
Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
[PMID: 8759170]
KB IC50
1.5 μg/mL
Compound: Velutin
Cytotoxicity against human KB cells after 72 hrs by neutral red assay
Cytotoxicity against human KB cells after 72 hrs by neutral red assay
[PMID: 10075750]
Neutrophil IC50
<= 12.51 μM
Compound: 59
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion generation pre-incubated for 2 mins prior to CB induction for 3 mins followed by fMLF addition by spectrophotometric analysis
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in superoxide anion generation pre-incubated for 2 mins prior to CB induction for 3 mins followed by fMLF addition by spectrophotometric analysis
[PMID: 37683361]
Neutrophil IC50
<= 15.25 μM
Compound: 59
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in elastase release using MeO-Suc-Ala-Ala-Pro-Val-pnitroanilide as substrate pre-incubated for 2 mins followed by fMLF/CB-stimulation and measured for 5 mins
Inhibition of fMLF/CB-induced human Neutrophil activation assessed as reduction in elastase release using MeO-Suc-Ala-Ala-Pro-Val-pnitroanilide as substrate pre-incubated for 2 mins followed by fMLF/CB-stimulation and measured for 5 mins
[PMID: 37683361]
P388 IC50
10 μg/mL
Compound: 4, Velutin
Cytotoxicity against mouse P388 cells
Cytotoxicity against mouse P388 cells
[PMID: 8759170]
In Vitro

Velutin (2-200 μM; 10 min) potently scavenges ABTS free radicals in cell-free assays, with an EC50 value of 23.13 μM[1].
Velutin (0.01-1 mM; pre-incubated with tyrosinase for 30 min, followed by incubation with L-tyrosine for 10 min) inhibits mushroom tyrosinase activity in cell-free assays, with an IC50 of 910.1 μM[1].
Velutin (10 μM; 48 h) completely inhibits α-MSH-induced melanogenesis in B16F10 mouse melanoma cells[1].
At a concentration of 10 μM with 48 h incubation, velutin inhibits α-MSH-induced intracellular tyrosinase activity by 87% in B16F10 mouse melanoma cells[1].
Velutin (0-32 μM; 24-48 h) exhibits no cytotoxicity toward primary mouse chondrocytes, with the maximum concentration reaching 32 μM after 24 h or 48 h of incubation[2].
Velutin (32 μM; 7 days) protects primary mouse chondrocytes against IL-1β-induced inflammation and extracellular matrix degradation after 7 days of incubation[2].
Velutin (4 μM; 2 h) inhibits RANKL-induced activation of p38 MAPK in mouse bone marrow-derived macrophages[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Velutin Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: B16F10 murine melanoma cells
Concentration: 20 μM
Incubation Time: 24 h
Result: Showed no significant cytotoxicity, with cell viability remaining near 100% at 20 μM.
In Vivo

Velutin (32 μM; intra-articular injection; once weekly for 8 consecutive weeks) exerts dual protective effects against articular cartilage degeneration and subchondral bone loss in DMM-induced osteoarthritis mice, reduces the OARSI score, increases the expression of aggrecan and type Ⅱ collagen, and inhibits the activation of p-p38 MAPK[2].
Velutin (i.p.; once daily; for 4 consecutive weeks; 5-10 mg/kg) dose-dependently alleviates intervertebral disc degeneration in a mouse model of lumbar instability[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 (7-week-old male, DMM-induced osteoarthritis)[2]
Dosage: 32 μM
Administration: intra-articular injection; once weekly; 8 weeks
Result: Showed significantly more regular articular cartilage surfaces, a thicker AC layer, a thinner calcified cartilage layer, and a lower osteophyte score.
Increased AC thickness, reduced CC thickness, and increased the AC/CC ratio.
Increased subchondral bone thickness, and significantly reduced the number of TRAP (+) multinucleated osteoclasts in SCB.
Increased aggrecan and type II collagen expression.
Reduced AC destruction and deeper Safranin O staining, with lower Osteoarthritis Research Society International scores.
Increased trabecular bone volume/tissue volume and trabecular number, and reduced trabecular separation in SCB, reducing SCB loss.
Significantly reduced p-p38 MAPK expression.
Molecular Weight

314.29

Formula

C17H14O6
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C1C=C(C2=CC=C(O)C(OC)=C2)OC3=CC(OC)=CC(O)=C13
Structure Classification
Initial Source

Flammulina velutipes
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (159.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1818 mL 15.9089 mL 31.8177 mL
5 mM 0.6364 mL 3.1818 mL 6.3635 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.60%

SDS (393 KB)

COA (248 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1818 mL 15.9089 mL 31.8177 mL 79.5444 mL
5 mM 0.6364 mL 3.1818 mL 6.3635 mL 15.9089 mL
10 mM 0.3182 mL 1.5909 mL 3.1818 mL 7.9544 mL
15 mM 0.2121 mL 1.0606 mL 2.1212 mL 5.3030 mL
20 mM 0.1591 mL 0.7954 mL 1.5909 mL 3.9772 mL
25 mM 0.1273 mL 0.6364 mL 1.2727 mL 3.1818 mL
30 mM 0.1061 mL 0.5303 mL 1.0606 mL 2.6515 mL
40 mM 0.0795 mL 0.3977 mL 0.7954 mL 1.9886 mL
50 mM 0.0636 mL 0.3182 mL 0.6364 mL 1.5909 mL
60 mM 0.0530 mL 0.2651 mL 0.5303 mL 1.3257 mL
80 mM 0.0398 mL 0.1989 mL 0.3977 mL 0.9943 mL
100 mM 0.0318 mL 0.1591 mL 0.3182 mL 0.7954 mL
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Velutin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Velutin25739-41-7Tyrosinasep38 MAPKNF-κBBacterialNuclear factor-κBNuclear factor-kappaBosteoclasttyrosinaseB16F10 murine melanoma cellsmelanogenesismouse nucleus pulposus cellsmushroom tyrosinaseAgaricus bisporus tyrosinaseMAPK pathwayNF-κB pathwayInhibitorinhibitorinhibit

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