α-Methyl-DL-tryptophan
Based on 1 Customer Validation
α-Methyl-DL-tryptophan (α-Methyltryptophan), a tryptophan derivative, is a selective SLC6A14 blocker. In estrogen receptor (ER)-positive breast cancer cells, α-Methyl-DL-tryptophan inhibits mTOR and activates autophagy and apoptosis. α-Methyl-DL-tryptophan also has the effect of reducing weight.
For research use only. We do not sell to patients.
- Purity: 99.99%
- CAS No.: 153-91-3
- Formula: C12H14N2O2
- Molecular Weight:218.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
α-Methyl-DL-tryptophan (α-MT) (2.5 mM; 24-72 h) increases the levels of asparagine synthetase and CHOP mRNA in MCF-7 cells. α-Methyl-DL-tryptophan does not increase the levels of these mRNAs in SLC6A14-negative MB-231 (a cancer cell line) and MCF-10A (a non-malignant cell line) cells[1].
α-Methyl-DL-tryptophan (2.5 mM; 48 h) induces cell autophagy in MCF-7 cells[1].
α-Methyl-DL-tryptophan (2.5 mM; 24-72 h) inhibits mTOR in MCF-7 cells[1].
α-Methyl-DL-tryptophan (2.5 mM; 48 h) induces apoptotic cell death in MCF-7 cells[1].
α-Methyl-DL-tryptophan (2.5 mM; 48 h) has no significant effect on cell migration and invasion in ZR-75-1 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 cells[1]
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Concentration:2.5 mM
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Incubation Time:24 h, 48 h, 72 h
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Result:Increased the levels of asparagine synthetase and CHOP mRNAs.
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Cell Line:MCF-7 cells[1]
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Concentration:2.5 mM
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Incubation Time:48 h
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Result:Induced autophagy as shown by punctate localization of constitutively expressed LC3 and presence of autophagosomes.
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Cell Line:MCF-7 cells[1]
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Concentration:2.5 mM
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Incubation Time:48 h
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Result:Apoptotic cell death was demonstrated by annexin V labeling and cleavage of lamin A.
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Cell Line:MCF-7 cells[1]
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Concentration:2.5 mM
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Incubation Time:24 h, 48 h, 72 h
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Result:Inhibited mTOR as evident from decreased phosphorylation of S6 and S6 kinase.
α-Methyl-DL-tryptophan (1 mg/mL; administered in drinking water; continuously administered; for 1 week) reduces body weight in wild-type mice on a high-fat diet[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude mice (female) inoculated with ZR-75-1 cells[1]
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Dosage:2 mg/mL
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Administration:Administered in drinking water
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Result:Reduced the growth of ZR-75-1 cells. Tumor size was significantly smaller than that in the control group.
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Animal Model:Wild-type C57BL/6 male mice fed a high-fat diet[2]
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Dosage:1 mg/mL
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Administration:Administered in drinking water, continuously for 1weeks
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Result:Led to a decrease in body weight.
Chemical Information
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CAS No. 153-91-3
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Appearance Solid
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Molecular Weight 218.26
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Formula C12H14N2O2
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Color White to light yellow
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Synonyms
α-Methyltryptophan
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
Ethanol : 10 mg/mL (45.82 mM; Need ultrasonic)
DMSO : 8.33 mg/mL (38.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (11.45 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (4.58 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (4.58 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1 mg/mL (4.58 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Karunakaran S, et al. SLC6A14 (ATB0,+) protein, a highly concentrative and broad specific amino acid transporter, is a novel and effective drug target for treatment of estrogen receptor-positive breast cancer. J Biol Chem. 2011 Sep 9;286(36):31830-8. [Content Brief]
[2]. Sathish Sivaprakasam, et al. α-Methyl-l-tryptophan as a weight-loss agent in multiple models of obesity in mice. Biochem J. 2021 Apr 16;478(7):1347-1358. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 4.5818 mL | 22.9089 mL | 45.8178 mL | 114.5444 mL |
| 5 mM | 0.9164 mL | 4.5818 mL | 9.1636 mL | 22.9089 mL | |
| 10 mM | 0.4582 mL | 2.2909 mL | 4.5818 mL | 11.4544 mL | |
| 15 mM | 0.3055 mL | 1.5273 mL | 3.0545 mL | 7.6363 mL | |
| 20 mM | 0.2291 mL | 1.1454 mL | 2.2909 mL | 5.7272 mL | |
| 25 mM | 0.1833 mL | 0.9164 mL | 1.8327 mL | 4.5818 mL | |
| 30 mM | 0.1527 mL | 0.7636 mL | 1.5273 mL | 3.8181 mL | |
| Ethanol | 40 mM | 0.1145 mL | 0.5727 mL | 1.1454 mL | 2.8636 mL |
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