25CN-NBOH hydrochloride

25CN-NBOH hydrochloride (2 h) is a high-affinity ligand for human 5-HT_2AR (K_i = 1.7 nM). In a [³H]Cimbi-36 competitive binding assay using membranes from tsA201 cells transfected with human receptors, 25CN-NBOH shows 76-fold selectivity for human 5-HT_2AR over human 5-HT_2CR^[1].
25CN-NBOH hydrochloride (1 h) is a potent partial agonist of human 5-HT_2AR (EC₅₀ = 2.4 nM, achieving 77% of the maximal response induced by 5-HT), while acting as a full agonist of human 5-HT_2CR (EC₅₀ = 46 nM, achieving 119% of the maximal response induced by 5-HT), as determined by an IP-One HTRF assay using stable HEK293 cell lines^[1].
25CN-NBOH hydrochloride (30 min) is a potent partial agonist of human 5-HT_2AR (EC₅₀ = 0.41 nM, achieving 80% of the maximal effect of 5-HT). It also acts as a partial agonist of human 5-HT_2CR (EC₅₀ = 5.8 nM, achieving 56% of the maximal effect of 5-HT) in a Ca²⁺/Fluo-4 assay using stable HEK293 cell lines^[1].
25CN-NBOH hydrochloride exhibits high in vitro binding selectivity for 5-HT_2AR, with 52-100-fold higher affinity for 5-HT_2AR than for 5-HT_2CR, 37-fold higher affinity than for 5-HT_2BR, and acts as a potent agonist of 5-HT_2AR^[2].
25CN-NBOH hydrochloride shows 100-fold binding selectivity for human 5-HT_2AR over rat 5-HT_2CR, with a K_i value of 1.3 nM for human 5-HT_2AR and 132 nM for rat 5-HT_2CR^[3].
25CN-NBOH hydrochloride (30 min) is a potent agonist with 90-fold functional selectivity for human 5-HT_2AR over human 5-HT_2CR. The EC₅₀ values are 2.1 nM for human 5-HT_2AR and 190 nM for human 5-HT_2CR^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.