5,7-Dihydroxytryptamine hydrobromide
5,7-Dihydroxytryptamine (5,7-DHT) hydrobromide is an autofluorescent (Ex ≈ 365 nm), selective neurotoxin and a transport substrate for MAO-A and 5-HT. 5,7-Dihydroxytryptamine hydrobromide can specifically target and damage central and peripheral 5-HTergic neurons, while affecting 5-HT-related pathways and neurotransmitter balance. 5,7-Dihydroxytryptamine hydrobromide can be used to establish 5-HTergic neuron injury models for studies on neural development, neurodegenerative diseases, as well as mechanisms related to platelet function and retinal neurons.
For research use only. We do not sell to patients.
- CAS No.: 1841081-30-8
- Formula: C10H13BrN2O2
- Molecular Weight:273.13
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
5,7-dihydroxytryptamine hydrobromide (25 μM; 60 min at 37°C) specifically labels serotonergic cells (but not dopaminergic cells) in 10-day-old primary mesencephalic cultures from 14-day-old Wistar rat foetuses[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
5,7-Dihydroxytryptamine (10 μg base; intracerebroventricular injection; single administration; 10 days prior to seizure induction) hydrobromide depletes 5-HT levels in the spinal cord of rats, but does not affect electrically induced spinal cord seizures in rats[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats (adult male, 175-200 g at surgery)[1]
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Dosage:8 μg
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Administration:bilateral stereotaxic infusion into nucleus accumbens; over 2 min, injector left in place for 1 additional min post-infusion
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Result:Increased mean amplitude of the acoustic startle reflex significantly across all stimulus intensities in the absence of background noise compared to the Sham-lesion group.
Showed no significant differences in startle amplitude between the DHT-lesion, Sham-lesion, and intact groups at any stimulus intensity in the presence of background noise.
Confirmed nearly complete loss of serotonergic neurons in the nucleus accumbens via immunohistochemistry.
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Animal Model:C57BL/6 (male, 2 and 10 months old, 20-30 g)[2]
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Dosage:12.5 μg; 25 μg; 50 μg
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Administration:i.c.v.; single dose
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Result:Had a damaging effect on the noradrenergic system, and this effect was independent of the age of the mice.
Exerted stronger toxic effects on serotonergic neurons in young individuals.
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Animal Model:M/511-Wistar (male, 240-280 g)[4]
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Dosage:4 µg (calculated as the base) per injection site
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Administration:stereotactic injection; 1 µl/min rate; needle left in situ for 1 min post-injection
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Result:Reduced ³H-5-HT uptake but did not change ³H-NA uptake in the cortex (medial bundle lesion group).
Reduced ³H-NA uptake to 75% of control (non-significant) in the cortex (medial plus lateral bundle lesion group).
Induced mouse killing behavior in 4 of 8 rats (medial bundle lesion group) and 6 of 7 rats (medial plus lateral bundle lesion group).
Required significantly more trials to reach habituation criterion for touch (both lesion groups).
Required significantly more trials for acoustic stimulation habituation (only medial plus lateral lesion group).
Showed a significantly higher number of boxing positions in the shock elicited fighting test (both lesion groups).
Showed no significant changes in home cage activity (both lesion groups).
Chemical Information
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CAS No. 1841081-30-8
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Molecular Weight 273.13
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Formula C10H13BrN2O2
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SMILES
NCCC1=CNC2=C(C=C(C=C12)O)O.Br
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Synonyms
5,7-DHP hydrobromide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Finnegan KT, et al. The amine-depleting effects of 5,7-dihydroxytryptamine (5,7-DHT) in C57BL/6 mice do not increase with age. Brain Res. 1989;496(1-2):251-256. [Content Brief]
[2]. Franke H, et al. 5,7-Dihydroxytryptamine--a selective marker of dopaminergic or serotonergic neurons?. Naunyn Schmiedebergs Arch Pharmacol. 2002;366(4):315-318. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
5,7-Dihydroxytryptamine hydrobromide Related Classifications
HY-N18471A Related Classifications
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