7-Ketocholesterol

Name: 7-Ketocholesterol
Brand: MedChemExpress
SKU: HY-113342
Rating: 5.0 (5 reviews)

Cat. No.: HY-113342 Purity: 99.87%: Data Sheet
COA

SDS
Handling Instructions
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7-Ketocholesterol is an oxidation product of cholesterol, widely present in atherosclerotic plaques, and has a stronger atherogenic effect than cholesterol. 7-Ketocholesterol can inhibit the rate-limiting enzymes involved in bile acid and cholesterol synthesis, such as cholesterol 7α-hydroxylase and HMG-CoA reductase. 7-Ketocholesterol exhibits pro-inflammatory effects both in vivo and in vitro and can induce cell apoptosis (apoptosis).

For research use only. We do not sell to patients.

CAS No. : 566-28-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of 7-Ketocholesterol:

7-Keto Cholesterol-d₇ In-stock
7-Ketocholesterol (Standard) Get quote

5 Publications Citing Use of MCE 7-Ketocholesterol

Flow Cytometry

In Vivo Efficacy Study

Cell Imaging/Staining

: 7-Ketocholesterol purchased from MedChemExpress. Usage Cited in: Nano Today. 2025 Oct.; Confocal fluorescence images and quantitative fluorescence analysis of the RAW264.7 cells incubated with fluorescently labeled 7-Ketocholesterol /Chol-doped BPSNs after siRNA knockdown of receptors (CD36, LOX-1, MSR1, and LDLR). Scale bar: 20 μm.

: 7-Ketocholesterol purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2025 Jul 23;11(1):338. [Abstract]; We also confirmed that the infiltration of M2 macrophages induced by RBM17-OE can be inhibited by T-CA inhibitors (7-Ketocholesterol, 10 μM, 24 h) in vivo experiment.

: 7-Ketocholesterol purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2025 Jul 23;11(1):338. [Abstract]; Comparing the effects of siHACD3 and 7-Ketocholesterol on serum FFA concentration in mice through in vivo experiments. Data was analyzed using t-tests. ns represents no significant difference.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
ARPE-19	IC₅₀	32.3 μM Compound: 4	Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
C6	IC₅₀	20 μM Compound: 7KC	Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method	[PMID: 24211631]
HEK293	EC₅₀	8.7 μM Compound: 9	Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy	[PMID: 24928400]
HEK293	EC₅₀	9.7 μM Compound: 6	Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis	[PMID: 23830695]
HT-29	IC₅₀	25.7 μM Compound: 4	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HT-29	IC₅₀	25.7 μM Compound: 6	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
Vero	IC₅₀	75 μM Compound: 1	Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay	[PMID: 24084159]

In Vitro

7-Ketocholesterol (50 μM, 2.5-2 hours) can enhance leukocyte-endothelial cell adhesion via the p38 MAPK pathway^[4].
7-Ketocholesterol (2.5-40 μg/mL, 24 hours) induces apoptosis in a dose-dependent manner by activating caspase-3/7, -8, and -12 in human microvascular endothelial cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:	Human umbilical vein endothelial cells (HUVECs)
Concentration:	50 μM
Incubation Time:	0.5-2 h
Result:	Stimulated the phosphorylation of p38 MAPK, reaching a peak within 30-120 minutes, with no significant effect on JNK. Enhanced the adhesion of THP-1 cells to HUVECs and upregulated the expression of E-selectin. Promoted the expression of E-selectin by activating the transcription factor ATF-2.

Cell Viability Assay^[5]

Cell Line:	Human umbilical vein endothelial cells (HUVECs)
Concentration:	2.5, 5, 10, 15, 20, 40 μg/mL
Incubation Time:	24 h
Result:	The average cell viability of HMVEC cells treated with 7-Ketocholesterol at concentrations of 40, 20, 10, 5, and 2.5 μg/ml for 24 hours was 45.33%, 72.83% (p < 0.001), 82.7% (p < 0.05), 88.47%, and 88.5% (p > 0.05), respectively, while the untreated control was 88.83%.

In Vivo

7-Ketocholesterol (5-20 mg/mL, administered via biodegradable wafers implanted into the anterior chamber of rats' eyes, single dose) exhibited pro-angiogenic and pro-inflammatory effects in Brown Norway rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Brown Norway rats (150-200 g)^[3]
Dosage:	5, 10, 15, 20 mg/mL
Administration:	Implanting biodegradable wafers containing 7-Ketocholesterol into the anterior chamber of rats' eyes, single dose
Result:	Significantly increased angiogenesis, with a large infiltration of macrophages detected by immunohistochemistry. Promoted the growth of new blood vessels originating from the limbus and extending through the cornea, peaking between days 7 and 10 after implantation. Led to a marked increase in vascular endothelial growth factor (VEGF), IL-1b, and GRO/KC in the aqueous humor.

Molecular Weight

400.64

Formula

C₂₇H₄₄O₂

CAS No.

566-28-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

C[C@]1(CC2)[C@](CC[C@]1([H])[C@H](C)CCCC(C)C)([H])[C@@](C(C=C3C[C@H]4O)=O)([H])[C@@]2([H])[C@]3(CC4)C

Structure Classification

Initial Source

Endogenous metabolite

human

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

Ethanol : 25 mg/mL (62.40 mM; Need ultrasonic)

DMSO : 6.06 mg/mL (15.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4960 mL	12.4800 mL	24.9601 mL
5 mM	0.4992 mL	2.4960 mL	4.9920 mL
10 mM	0.2496 mL	1.2480 mL	2.4960 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.62 mg/mL (1.55 mM); Clear solution

This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.61 mg/mL (1.52 mM); Clear solution

This protocol yields a clear solution of ≥ 0.61 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.61 mg/mL (1.52 mM); Clear solution

This protocol yields a clear solution of ≥ 0.61 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.1 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (248 KB) HNMR (262 KB) RP-HPLC (1247 KB) LCMS (133 KB)

Handling Instructions (2659 KB)

References

[1]. M A Lyons, et al. 7-Ketocholesterol. Int J Biochem Cell Biol. Mar-Apr 1999;31(3-4):369-75. [Content Brief]

[2]. Amelia Anderson, et al. 7-Ketocholesterol in disease and aging. Redox Biol. 2020 Jan;29:101380. [Content Brief]

[3]. Amaral J, et al. 7-Ketocholesterol induces inflammation and angiogenesis in vivo: a novel rat model. PLoS One. 2013;8(2):e56099. [Content Brief]

[4]. Tani M, et al. 7-Ketocholesterol enhances leukocyte adhesion to endothelial cells via p38MAPK pathway. PLoS One. 2018 Jul 31;13(7):e0200499. [Content Brief]

[5]. Luthra S, et al. 7-Ketocholesterol activates caspases-3/7,-8, and-12 in human microvascular endothelial cells in vitro[J]. Microvascular research, 2008, 75(3): 343-350. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	2.4960 mL	12.4800 mL	24.9601 mL	62.4002 mL
	5 mM	0.4992 mL	2.4960 mL	4.9920 mL	12.4800 mL
	10 mM	0.2496 mL	1.2480 mL	2.4960 mL	6.2400 mL
	15 mM	0.1664 mL	0.8320 mL	1.6640 mL	4.1600 mL
Ethanol	20 mM	0.1248 mL	0.6240 mL	1.2480 mL	3.1200 mL
	25 mM	0.0998 mL	0.4992 mL	0.9984 mL	2.4960 mL
	30 mM	0.0832 mL	0.4160 mL	0.8320 mL	2.0800 mL
	40 mM	0.0624 mL	0.3120 mL	0.6240 mL	1.5600 mL
	50 mM	0.0499 mL	0.2496 mL	0.4992 mL	1.2480 mL
	60 mM	0.0416 mL	0.2080 mL	0.4160 mL	1.0400 mL