AKT Kinase Inhibitor
Based on 18 publication(s) in Google Scholar
AKT Kinase Inhibitor is an Akt kinase inhibitor with anti-tumor activity.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 842148-40-7
- Formula: C16H19N7O3
- Molecular Weight:357.37
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) AKT Kinase Inhibitor
More- Cell. 2026 Jun 11;189(12):3541-3552.e18. [Abstract]
- Cell Stem Cell. 2023 Apr 6;30(4):450-459.e9. [Abstract]
- Small. 2023 Apr;19(16):e2207194. [Abstract]
- Pharmacol Res. 2024 May 18:107218. [Abstract]
- Int J Biol Sci. 2024 Apr 29;20(7):2748-2762. [Abstract]
- Cell Death Dis. 2024 Nov 9;15(11):809. [Abstract]
- Cell Commun Signal. 2022 Mar 19;20(1):35. [Abstract]
- Cell Rep. 2023 May 23;42(6):112547. [Abstract]
- Phytother Res. 2022 Apr;36(4):1724-1735. [Abstract]
- Transl Psychiatry. 2026 Apr 1;16(1):275. [Abstract]
- Life Sci. 2019 Aug 15:231:116554. [Abstract]
- Biofactors. 2025 May-Jun;51(3):e70023. [Abstract]
- Inflammation. 2021 Jun;44(3):890-898. [Abstract]
- Mediators Inflamm. 2020 Jun 24;2020:3621261. [Abstract]
- J Cancer. 2021 May 5;12(13):3809-3818. [Abstract]
- Research Square Preprint. 2023 May 26.
- Oxid Med Cell Longev. 2021 Mar 19:2021:5595376. [Abstract]
- Research Square Preprint. 2020 Dec.
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WB
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RT-PCR
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WB
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IF
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Cell Migration/Invasion Assay
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BT-474 | IC50 |
1.13 μM
Compound: 12c
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Inhibition of AKT-mediated GSK3-beta phosphorylation in human BT474 cells
Inhibition of AKT-mediated GSK3-beta phosphorylation in human BT474 cells
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[PMID: 18800763] |
| Sf9 | IC50 |
0.02 μM
Compound: 12c
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Inhibition of human recombinant ROCK1 expressed in Sf9 cells
Inhibition of human recombinant ROCK1 expressed in Sf9 cells
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[PMID: 18800763] |
The effect of selective inhibition of Akt in proliferating cells expressing Trop-2 is studied. Akt inhibition, either by silencing or with specific drugs, induces a marked decrease in cell proliferation in cells expressing Trop-2, in a dose-dependent fashion[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 842148-40-7
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Appearance Solid
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Molecular Weight 357.37
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Formula C16H19N7O3
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Color White to light yellow
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SMILES
OCC#CC1=NC=C(C2=C1N=C(N2CC)C3=NON=C3N)OCCCN
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (18)
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Journal Impact Factor
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Most Recent
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Cell
2026 Jun 11;189(12):3541-3552.e18. PMID: 41999746 -
Cell Stem Cell
Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming. [Abstract]2023 Apr 6;30(4):450-459.e9. PMID: 36944335
AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2023 Apr 6;30(4):450-459.e9. [Abstract]
Immunofluorescence of LIN28A at the end of stage I under SI_BMP4/B27+AKTi (AKT Kinase Inhibitor: 1 μM) +VTP+J condition.
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Small
2023 Apr;19(16):e2207194. PMID: 36634971
AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Small. 2023 Apr;19(16):e2207194. [Abstract]
AKT Kinase Inhibitor (10 μM). Comparison of migration restriction ability of SK-Hep-1, SNU-449, PANC-1, SW1990 and primary cancer-associated fibroblasts.
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Pharmacol Res
STC1 competitively binding βPIX enhances melanoma progression via YAP nuclear translocation and M2 macrophage recruitment through the YAP/CCL2/VEGFA/AKT feedback loop. [Abstract]2024 May 18:107218. PMID: 38768671
AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 May 18:107218. [Abstract]
Stimulation of B16-F10 cells with TAM-conditioned medium, pre-treated with AKT Kinase Inhibitor (5 μM) for 24 h. Western blot assessing AKT phosphorylation, AKT baseline expression, and STC1 expression.
AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 May 18:107218. [Abstract]
Stimulation of B16-F10 cells with TAM-conditioned medium, pre-treated with AKT kinase inhibitor (5 μM) for 24 h. CCL2 mRNA expression was detected by qPCR.
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Int J Biol Sci
Nuclear Softness Promotes the Metastatic Potential of Large-Nucleated Colorectal Cancer Cells via the ErbB4-Akt1-Lamin A/C Signaling Pathway. [Abstract]2024 Apr 29;20(7):2748-2762. PMID: 38725859
AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 29;20(7):2748-2762. [Abstract]
Western blotting analysis of Akt1, p-Akt1 (Ser473), lamin A/C, and p-lamin A/C (Ser22) in SW480 LNCs treated with or without AKT Kinase Inhibitor (Akti; 0.5 μM).
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Cell Death Dis
Combined metformin and simvastatin therapy inhibits SREBP2 maturation and alters energy metabolism in glioma. [Abstract]2024 Nov 9;15(11):809. PMID: 39521788 -
Cell Commun Signal
Down-regulation of kappa opioid receptor promotes ESCC proliferation, invasion and metastasis via the PDK1-AKT signaling pathway. [Abstract]2022 Mar 19;20(1):35. PMID: 35305679 -
Cell Rep
2023 May 23;42(6):112547. PMID: 37224020 -
Phytother Res
Maltol mitigates cisplatin-evoked cardiotoxicity via inhibiting the PI3K/Akt signaling pathway in rodents in vivo and in vitro. [Abstract]2022 Apr;36(4):1724-1735. PMID: 35174550 -
Transl Psychiatry
Non-gene-edited neural stem cells reverse neuroinflammation and microbiota dysbiosis in a sprague-dawley rat model of autism spectrum disorder. [Abstract]2026 Apr 1;16(1):275. PMID: 41922322 -
Life Sci
Asprosin improves the survival of mesenchymal stromal cells in myocardial infarction by inhibiting apoptosis via the activated ERK1/2-SOD2 pathway. [Abstract]2019 Aug 15:231:116554. PMID: 31194992 -
Biofactors
The Immunotherapeutic Target ANLN Promotes Renal Clear Cell Carcinoma by Regulating PI3K/Akt and P53 Signaling Pathways. [Abstract]2025 May-Jun;51(3):e70023. PMID: 40411279 -
Inflammation
Tadalafil Alleviates LPS-Induced Inflammation and Oxidative Stress of RWPE-1 Cell by Regulating the Akt/Nrf2 Signaling Pathway. [Abstract]2021 Jun;44(3):890-898. PMID: 33398543 -
Mediators Inflamm
Secoisolariciresinol Diglucoside Exerts Anti-Inflammatory and Antiapoptotic Effects through Inhibiting the Akt/I κ B/NF- κ B Pathway on Human Umbilical Vein Endothelial Cells. [Abstract]2020 Jun 24;2020:3621261. PMID: 32684834
AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2020 Jun 24;2020:3621261. [Abstract]
After treatment with HY-10249A, the expression of p-IκB-α and p-NF-κB p65 is reduced.
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J Cancer
YTHDF2 inhibit the tumorigenicity of endometrial cancer via downregulating the expression of IRS1 methylated with m6A. [Abstract]2021 May 5;12(13):3809-3818. PMID: 34093789 -
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Oxid Med Cell Longev
A Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting Melatonin Secretion. [Abstract]2021 Mar 19:2021:5595376. PMID: 33828639 -
Solvent & Solubility
DMSO : 20 mg/mL (55.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.67 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7982 mL | 13.9911 mL | 27.9822 mL | 69.9555 mL |
| 5 mM | 0.5596 mL | 2.7982 mL | 5.5964 mL | 13.9911 mL | |
| 10 mM | 0.2798 mL | 1.3991 mL | 2.7982 mL | 6.9956 mL | |
| 15 mM | 0.1865 mL | 0.9327 mL | 1.8655 mL | 4.6637 mL | |
| 20 mM | 0.1399 mL | 0.6996 mL | 1.3991 mL | 3.4978 mL | |
| 25 mM | 0.1119 mL | 0.5596 mL | 1.1193 mL | 2.7982 mL | |
| 30 mM | 0.0933 mL | 0.4664 mL | 0.9327 mL | 2.3319 mL | |
| 40 mM | 0.0700 mL | 0.3498 mL | 0.6996 mL | 1.7489 mL | |
| 50 mM | 0.0560 mL | 0.2798 mL | 0.5596 mL | 1.3991 mL |