2. NF-κB
  3. NF-κB
  4. Arctiin

Arctiin  (Synonyms: Arctii; NSC 315527; Arctigenin-4-glucoside)

Cat. No.: HY-N0034 Purity: 99.52%
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Arctiin is an orally active inhibitor of NF-κB. Arctiin suppresses cyclin D1 protein expression in human tumor cells. Arctiin also reduces malondialdehyde and pro-in ammatory cytokines levels. Arctiin can used in study glomerulonephritis.

For research use only. We do not sell to patients.

CAS No. : 20362-31-6

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10 mM * 1 mL in DMSO
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Arctiin:

Arctiin Related Antibodies

Top Publications Citing Use of Products

    Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927.  [Abstract]

    Protein immunoblot analysis was performed to examine the expression of AdipoR1 in the retina of EAU mice at day 20 treated with Arctiin (1, 10 μM).

    Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927.  [Abstract]

    The mRNA expression of T cell-related inflammatory factors in the retina was determined by RT-PCR treated with Arctiin (10 μM).

    Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927.  [Abstract]

    CD4+ T cells were treated with DMSO or Arctiin (0.1, 1 and 10 μM) for 48 h, then cytotoxicity of Arctiin was detected by apoptosis assay.

    Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927.  [Abstract]

    The protein expression of IFN-γ, IL-17 A and IL-10 in culture supernatant was determined by ELISA treated with Arctiin (10 μM).

    Arctiin purchased from MedChemExpress. Usage Cited in: Cell Immunol. 2025 Jan 16:409-410:104927.  [Abstract]

    CFSE labled CD4+ T cells were treated with DMSO or Arctiin (10 μM) for 96 h, then cell proliferation was detected by flow cytometry.

    View All NF-κB Isoform Specific Products:

    View All Isoforms
    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Arctiin is an orally active inhibitor of NF-κB. Arctiin suppresses cyclin D1 protein expression in human tumor cells. Arctiin also reduces malondialdehyde and pro-in ammatory cytokines levels. Arctiin can used in study glomerulonephritis[4][6].

    Cellular Effect
    Cell Line Type Value Description References
    MRC5 CC50
    240 μM
    Compound: 8
    Cytotoxicity against MRC5 cells
    Cytotoxicity against MRC5 cells
    		[PMID: 17239594]
    MRC5 IC50
    16 μM
    Compound: 8
    Antiviral activity against HCMV in MRC5 cells by plaque reduction assay
    Antiviral activity against HCMV in MRC5 cells by plaque reduction assay
    		[PMID: 17239594]
    RAW264.7 IC50
    > 0.3 mM
    Compound: 22
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs
    		[PMID: 18986199]
    In Vitro

    Arctiin (100 μM) down-regulates cyclin D1 protein expression in human tumor cells[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Arctiin Related Antibodies

    Cell Differentiation Assay[6]

    Cell Line: HaCaT, MG63, A549, HCT116, HeLa, MCF-7, and 293T cell line
    Concentration: 10, 25,100 and 250 µM
    Incubation Time: 0, 6, 12, 24 and 48 h
    Result: Inhibited the HaCaT cells growth with 100 µM at 48 h.
    Significantly repressed the expression of the cyclin D1 protein with 100 µM in all tumor cells.
    Dephosphorylated RB protein in HaCaT cells at 48 h.
    In Vivo

    Arctiin (120 mg/kg for p.o.;Once daily for 3 weeks) significantly improves cBSA-induced glomerulonephritis in glomerulonephritis rat model[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Glomerulonephritis rat model[4]
    Dosage: 30, 60, and 120 mg/kg
    Administration: Oral gavage (p.o.);Once daily for 3 weeksv
    Result: Significantly decreased the levels of urine protein.
    Markedly ameliorated the renal function.
    Dramatically reduced IL-6 and TNF-a concentration in rat serum.
    Dramatically decreased the NF-kB DNA binding activity.
    Significantly reduced the MDA level and increased the SOD activity.
    Molecular Weight

    534.55

    Formula

    C27H34O11
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](OC2=C(OC)C=C(C[C@H]3C(OC[C@@H]3CC4=CC(OC)=C(OC)C=C4)=O)C=C2)[C@@H]1O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 200 mg/mL (374.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 10 mg/mL (18.71 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8707 mL 9.3537 mL 18.7073 mL
    5 mM 0.3741 mL 1.8707 mL 3.7415 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.89 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 10 mg/mL (18.71 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.52%

    SDS (393 KB)

    COA (259 KB) LCMS (88 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.8707 mL 9.3537 mL 18.7073 mL 46.7683 mL
    5 mM 0.3741 mL 1.8707 mL 3.7415 mL 9.3537 mL
    10 mM 0.1871 mL 0.9354 mL 1.8707 mL 4.6768 mL
    15 mM 0.1247 mL 0.6236 mL 1.2472 mL 3.1179 mL
    DMSO 20 mM 0.0935 mL 0.4677 mL 0.9354 mL 2.3384 mL
    25 mM 0.0748 mL 0.3741 mL 0.7483 mL 1.8707 mL
    30 mM 0.0624 mL 0.3118 mL 0.6236 mL 1.5589 mL
    40 mM 0.0468 mL 0.2338 mL 0.4677 mL 1.1692 mL
    50 mM 0.0374 mL 0.1871 mL 0.3741 mL 0.9354 mL
    60 mM 0.0312 mL 0.1559 mL 0.3118 mL 0.7795 mL
    80 mM 0.0234 mL 0.1169 mL 0.2338 mL 0.5846 mL
    100 mM 0.0187 mL 0.0935 mL 0.1871 mL 0.4677 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Arctiin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Arctiin20362-31-6Arctii NSC 315527 Arctigenin-4-glucosideNSC315527NSC 315527NSC-315527NF-κBNuclear factor-κBNuclear factor-kappaBNuclear factor-kBMCF-7Cyclin D1 proteinMDAIL-6TNF-aGlomerulonephritisPC-3Inhibitorinhibitorinhibit

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