Cidofovir (Synonyms: GS 0504; HPMPC; (S)-HPMPC)

Name: Cidofovir
Brand: MedChemExpress
SKU: HY-17438
Rating: 5.0 (14 reviews)

Cat. No.: HY-17438 Purity: 99.95%: Data Sheet
COA

SDS
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Cidofovir (GS 0504) is an acyclic monophosphate nucleotide analogue and CMV inhibitor with antiviral activity. Cidofovir inhibits cytomegalovirus (CMV) replication by selectively inhibiting viral DNA polymerase. Cidofovir induces apoptosis and can be used in studies of AIDS cytomegalovirus retinitis, herpes, and cancer. Cidofovir also has anti-orthopoxvirus and anti-variola activities.

For research use only. We do not sell to patients.

CAS No. : 113852-37-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of Cidofovir:

14 Publications Citing Use of MCE Cidofovir

Cell Proliferation/Viability Assay

RT-PCR

: Cidofovir purchased from MedChemExpress. Usage Cited in: Pharmaceuticals (Basel). 2025 Oct 16;18(10):1560. [Abstract]; The cytotoxicity of three reference compounds (GCV, CDV (Cidofovir), and BCV) on COS-7 cells was tested.

: Cidofovir purchased from MedChemExpress. Usage Cited in: Virol Sin. 2025 Mar 25:S1995-820X(25)00031-8. [Abstract]; HEK293T cells were transfected with pHBoV1-HiBiTNS1 in the presence of Cidofovir (200 μM), favipiravir (50 μM), ivermectin (10 μM), nevirapine (50 μM), remdesivir (10 μM), ribavirin (50 μM), sofosbuvir (50 μM), or zidovudin (50 μM). At 72 h post-transfection, the Nano-Glo luciferase assay was performed.

: Cidofovir purchased from MedChemExpress. Usage Cited in: J Virol. 2025 Jan 23:e0153924. [Abstract]; Antiviral activity of cidofovir against HBoV1. MA104 cells were treated with 50, 100, 200, 400 µM cidofovir for 6 h and then infected with HBoV1 for 12 h, and the intracellular viral DNA copies were detected at 96 h.p.i.

: Cidofovir purchased from MedChemExpress. Usage Cited in: Poult Sci. 2024 Dec;103(12):104238. [Abstract]; DEFs infected DPV at MOI=1, then treated with Cidofovir (10 nmol) for 1 h, 2 h, 3 h, 4 h, 6 h, 8 h, 10 h, 12 h, 14 h and 16 h, qRT-PCR detected DPV copy numbers in the supernatant.

: Cidofovir purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2023 Dec;12(1):2208682. [Abstract]; Cytotoxicity of Cidofovir and its Inhibition efficiency on WIBP-MPXV-001

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cidofovir (GS 0504) is an acyclic monophosphate nucleotide analogue and CMV inhibitor with antiviral activity. Cidofovir inhibits cytomegalovirus (CMV) replication by selectively inhibiting viral DNA polymerase. Cidofovir induces apoptosis and can be used in studies of AIDS cytomegalovirus retinitis, herpes, and cancer^[1]^[3]. Cidofovir also has anti-orthopoxvirus and anti-variola activities^[4].

IC₅₀ & Target

DNA Polymerase

Cellular Effect

Cell Line	Type	Value	Description	References
ARPE-19	CC₅₀	> 100 μM Compound: CDV	Cytotoxicity against human ARPE-19 cells assessed as reduction in cell viability treated for 72 hrs by neutral red assay Cytotoxicity against human ARPE-19 cells assessed as reduction in cell viability treated for 72 hrs by neutral red assay	[PMID: 37140467]
HEL	CC₅₀	64 μM Compound: Cidofovir	Cytostatic activity against human HEL cells assessed as reduction in cell growth measured after 24 hrs Cytostatic activity against human HEL cells assessed as reduction in cell growth measured after 24 hrs	[PMID: 38643669]
HFF	CC₅₀	> 150 μM Compound: 34	Cytotoxicity against human HFF cells assessed as cell growth inhibition by celltiter-glo assay Cytotoxicity against human HFF cells assessed as cell growth inhibition by celltiter-glo assay	[PMID: 38784469]

In Vitro

Cidofovir (5-100 μM, 72 hours) has antiviral activity against feline herpesvirus type-1 (FHV-1) with an IC₅₀ of 11 μM, and can reduce Crandell-Reese feline kidney cell counts in a dose-dependent manner^[1].
Cidofovir (10-1000 μM, 24-120 hours) can reduce cancer cell viability and induces apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	Crandell-Reese feline kidney(CRFK) cells
Concentration:	10-100 μM
Incubation Time:	72 hours
Result:	Reduced CRFK cells by 9.1%.

Cell Viability Assay^[3]

Cell Line:	Caco-2, FTC-133, HeLa, Hep-G2, MDA-MB-231, NCI-H1975 and PC-3 cells
Concentration:	10-1000 μM
Incubation Time:	24, 48, 72, 96, 120 hours
Result:	Resulted in a gradual decrease in tumor cell viability with time and concentration increasing and inhibited the number of FTC-133 cell clones by about 55% at 100 μM comparing to the untreated group.

Apoptosis Analysis^[3]

Cell Line:	FTC-133 cells
Concentration:	100 μM
Incubation Time:	96 hours
Result:	Showed a significant increase in the expression of pro-apoptotic proteins, such as cytochrome c, phospho-p53 (S15) and caspase-3 by 130%, 49%, and 46%, respectively while the anti-apoptotic protein Bcl-x decreased significantly by 57% comparing to the untreated cells.

In Vivo

Cidofovir (subcutaneous injection, 100 mg/kg, 3-6 days interval, 21 days) is highly protective against death from cowpox virus (CPV) infection at high doses in female weanling BALB/c mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female weanling BALB/c mice infected with cowpox virus (CPV)^[2]
Dosage:	100 mg/kg
Administration:	Subcutaneous injection; 3-6 days interval; 21 days
Result:	Prevented 80-100% of mouse deaths when administered on the first 4-3 days before infection. Protected 35-50% of mice when administered on the fourth day after infection, and 10-20% when administered on the sixth day.

Molecular Weight

279.19

Formula

C₈H₁₄N₃O₆P

CAS No.

113852-37-2

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H](OCP(O)(O)=O)CN1C=CC(N)=NC1=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 3.33 mg/mL (11.93 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5818 mL	17.9090 mL	35.8179 mL
5 mM	0.7164 mL	3.5818 mL	7.1636 mL
10 mM	0.3582 mL	1.7909 mL	3.5818 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 4.55 mg/mL (16.30 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (281 KB) SDS (480 KB)

COA (253 KB) HNMR (259 KB) CNMR (260 KB) RP-HPLC (205 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. David J Maggs, et al. In vitro efficacy of ganciclovir, cidofovir, penciclovir, foscarnet, idoxuridine, and acyclovir against feline herpesvirus type-1. Am J Vet Res. 2004 Apr;65(4):399-403. [Content Brief]

[2]. M Bray, et al. Cidofovir protects mice against lethal aerosol or intranasal cowpox virus challenge. J Infect Dis. 2000 Jan;181(1):10-9. [Content Brief]

[3]. Simona Catalani, et al. Reduced cell viability and apoptosis induction in human thyroid carcinoma and mesothelioma cells exposed to cidofovir. Toxicol In Vitro. 2017 Jun;41:49-55. [Content Brief]

[4]. Robert O Baker, et al. Potential antiviral therapeutics for smallpox, monkeypox and other orthopoxvirus infections. Antiviral Res. 2003 Jan;57(1-2):13-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.5818 mL	17.9090 mL	35.8179 mL	89.5448 mL
	5 mM	0.7164 mL	3.5818 mL	7.1636 mL	17.9090 mL
	10 mM	0.3582 mL	1.7909 mL	3.5818 mL	8.9545 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Cidofovir Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cidofovir (Synonyms: GS 0504; HPMPC; (S)-HPMPC)

Customer Review

Other Forms of Cidofovir:

Cidofovir Related Antibodies

14 Publications Citing Use of MCE Cidofovir

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Cidofovir Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Cidofovir Related Classifications

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