Daidzin (Synonyms: Daidzoside; NPI-031D; Daidzein 7-O-glucoside)

Name: Daidzin
Brand: MedChemExpress
SKU: HY-N0018
Rating: 5.0 (12 reviews)

Cat. No.: HY-N0018 Purity: 99.77%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Daidzin is an isoflavone with antioxidant, anticancer, and antiatherosclerotic activities. Daidzin is a potent and selective inhibitor of mitochondrial ALDH-2. Daidzin reduces ethanol consumption.

For research use only. We do not sell to patients.

CAS No. : 552-66-9

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Daidzin:

Daidzin (Standard) In-stock

12 Publications Citing Use of MCE Daidzin

Cell Imaging/Staining

Bio/Physico-chemical Assay

Flow Cytometry

In Vivo Imaging

: Daidzin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 4:e2408373. [Abstract]; MM1S cells were treated with 6‐MP or Daidzin (0-20 μM) at the indicated concentrations for 48 h and the expression of CAV1 was detected using western blot.

: Daidzin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 4:e2408373. [Abstract]; MM1S cells were treated with Bortezomib (1.25 nM) in combination with 6‐MP or Daidzin (20 μM) for 48 h and cell apoptosis was determined using flow cytometry.

: Daidzin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 4:e2408373. [Abstract]; B-NDG mice were inoculated with MM1S-Luc cells and randomly divided into six groups (n = 5-7). After 11 days of inoculation, these mice were intraperitoneally injected with 6-MP (10-20 mg/kg), Daidzin (30 mg/kg), or vehicle daily until day 67. After 15 days of inoculation, mice were intraperitoneally injected with bortezomib (0.5 mg/kg) three times a week until day 67. The distribution of MM1S-Luc cells in these mice was measured at the indicated days after inoculation using a living imaging system.

: Daidzin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 May;21(5):510-526. [Abstract]; Representative immunofluorescence images showing NET formation in human peripheral blood neutrophils (Daidzin (20 μM)).

: Daidzin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 May;21(5):510-526. [Abstract]; The accumulation of H3Cit was significantly decreased by Alda-1 treatment and increased by Daidzin (20 μM) treatment, as observed in both ALDH2^wt and ALDH2^rs671 mutant neutrophils.

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View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

View All Isoforms

ALDH1 ALDH2 ALDH3

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	61.2 μM Compound: 1	Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24974349]

In Vitro

Daidzin inhibits hamster and rat ALDH-2, with K_is of 0.082 and 0.052 μM respectively^[1].
Daidzin (500 μM) shows antioxidant activity, measured by its ability to inhibit cytochrome c reduction by Phorbol myristic acetate (PMA)-stimulated neutrophils^[3].
Daidzin (0-30 μM, 30 min) inhibits the formation of 5-HIAA (IC₅₀ value of ≈2.7 μM), and increases the amounts of 5-HIAL and 5-HTOL in the incubation media of hamster liver mitochondrial^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Daidzin (30 mg/kg, intragastrically) decreases blood alcohol levels (induced by 3 g ethanol/kg body weight), and shortens sleep time induced by Ethanol intoxication (7 g/kg by intubation) in fasted rats^[3].
Daidzin (70 meq/hamster/day, i.p.) inhibits hamster ethanol intake by 62%^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

416.38

Formula

C₂₁H₂₀O₉

CAS No.

552-66-9

Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(C2=COC(C=C(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)C=C4)=C4C2=O)C=C1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 200 mg/mL (480.33 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4017 mL	12.0083 mL	24.0165 mL
5 mM	0.4803 mL	2.4017 mL	4.8033 mL
10 mM	0.2402 mL	1.2008 mL	2.4017 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.00 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 25 mg/mL (60.04 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 0.5% CMC-Na/1% Tween-80 in Saline water
Solubility: 39.6 mg/mL (95.11 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (251 KB) HNMR (191 KB) LCMS (110 KB)

Handling Instructions (2659 KB)

References

[1]. Xie CI, et al. Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication. Alcohol Clin Exp Res. 1994 Dec;18(6):1443-7. [Content Brief]

[2]. Keung WM, et al. Kudzu root: an ancient Chinese source of modern antidipsotropic agents. Phytochemistry. 1998 Feb;47(4):499-506. [Content Brief]

[3]. Keung WM, et al. Daidzin inhibits mitochondrial aldehyde dehydrogenase and suppresses ethanol intake of Syrian golden hamsters. Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1675-9. [Content Brief]

[4]. Keung WM, Vallee BL. Daidzin and its antidipsotropic analogs inhibit serotonin and dopamine metabolism in isolated mitochondria. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2198-203. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4017 mL	12.0083 mL	24.0165 mL	60.0413 mL
	5 mM	0.4803 mL	2.4017 mL	4.8033 mL	12.0083 mL
	10 mM	0.2402 mL	1.2008 mL	2.4017 mL	6.0041 mL
	15 mM	0.1601 mL	0.8006 mL	1.6011 mL	4.0028 mL
	20 mM	0.1201 mL	0.6004 mL	1.2008 mL	3.0021 mL
	25 mM	0.0961 mL	0.4803 mL	0.9607 mL	2.4017 mL
	30 mM	0.0801 mL	0.4003 mL	0.8006 mL	2.0014 mL
	40 mM	0.0600 mL	0.3002 mL	0.6004 mL	1.5010 mL
	50 mM	0.0480 mL	0.2402 mL	0.4803 mL	1.2008 mL
	60 mM	0.0400 mL	0.2001 mL	0.4003 mL	1.0007 mL
	80 mM	0.0300 mL	0.1501 mL	0.3002 mL	0.7505 mL
	100 mM	0.0240 mL	0.1201 mL	0.2402 mL	0.6004 mL

Daidzin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Daidzin552-66-9Daidzoside NPI-031D Daidzein 7-O-glucosideMitochondrial MetabolismReverse TranscriptaseAldehyde Dehydrogenase (ALDH)isoflavone with antioxidantanticancerantiatherosclerotichamsterInhibitorinhibitorinhibit

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Daidzin (Synonyms: Daidzoside; NPI-031D; Daidzein 7-O-glucoside)

Customer Review

Other Forms of Daidzin:

Daidzin Related Antibodies

12 Publications Citing Use of MCE Daidzin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Aldehyde Dehydrogenase (ALDH) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Daidzin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Daidzin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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