Fomepizole
Based on 4 publication(s) in Google Scholar
Fomepizole (4-Methylpyrazole) is a potent cytochrome P450 (CYP2E1) inhibitor. Fomepizole is a competitive inhibitor of the enzyme alcohol dehydrogenase. Fomepizole blocks further conversion of methanol and ethylene glycol to toxic metabolites. Fomepizole has the potential for an antidote for ethylene glycol or methanol poisoning.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.78%
- CAS. Nr.: 7554-65-6
- Formel: C4H6N2
- Molecular Weight:82.11
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Speicherung:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Chemical Information
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Lösungsmittel & Löslichkeit
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Publications Citing Use of MedChemExpress (MCE) Fomepizole
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Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biologische Aktivität
Beschreibung
IC50 & Target
[3]|
CYP2E1 |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male CD-1 mice weighing 18-25 g[4]
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Dosage:25 mg/kg
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Administration:IP; single dose
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Result:Decreased the dose of ethanol (1-5 g/kg; IP) at which 50% of the animals failed a particular outcome test (toxic dose 50; TD50).
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS. Nr. 7554-65-6
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Appearance Liquid (Density: 0.993 g/cm3)
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Molecular Weight 82.11
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Formel C4H6N2
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Color Off-white to light yellow
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SMILES
CC1=CNN=C1
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Synonyms
4-Methylpyrazole
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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Plant Physiol
Symbiosis between Dendrobium catenatum protocorms and Serendipita indica involves the plant hypoxia response pathway. [Abstract]2023 Jul 3;192(3):2554-2568. PMID: 36988071
Fomepizole purchased from MedChemExpress. Usage Cited in: Plant Physiol. 2023 Jul 3;192(3):2554-2568. [Abstract]
The ADH inhibitors 4-methylpyrazole inhibits the NADH-converting activities of DcADH1 and DcADH2 in vitro.
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Anal Chem
A Computational Integration Strategy Driven by Chemical Similarity Uncovers Comprehensive Metabolic Profiles of Small Bioactive Peptides via UHPLC-HRMS for Doping Control. [Abstract]2025 Nov 18;97(45):25158-25167. PMID: 41202118 -
Food Chem Toxicol
The "steric-like" inhibitory effect and mechanism of doxycycline on florfenicol metabolism: Interaction risk. [Abstract]2022 Nov:169:113431. PMID: 36116547
Fomepizole purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2022 Nov:169:113431. [Abstract]
The influence of specific inhibitors of CYP450 [sulphaphenazole (CYP2C9 inhibitor), quinidine (CYP2D6 inhibitor), fomepizole (CYP2E1 inhibitor), methoxsalen (CYP2A6 inhibitor), diosmetin (CYP1A inhibitor) and ketoconazol (CYP3A4 inhibitor)] on the generation of FFA.
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Chemosphere
A proposed "steric-like effect" for the slowdown of enrofloxacin antibiotic metabolism by ciprofloxacin, and its mechanism. [Abstract]2021 Dec:284:131347. PMID: 34323809
Lösungsmittel & Löslichkeit
In Vitro:
H2O : 100 mg/mL (1217.88 mM; Need ultrasonic)
DMSO : ≥ 100 mg/mL (1217.88 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (30.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (30.45 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 140 mg/mL (1705.03 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Casavant MJ. Fomepizole in the treatment of poisoning. Pediatrics. 2001 Jan;107(1):170. [Content Brief]
[2]. Lepik KJ, et al. Adverse drug events associated with the antidotes for methanol and ethylene glycol poisoning: a comparison of ethanol and fomepizole. Ann Emerg Med. 2009 Apr;53(4):439-450.e10. [Content Brief]
[4]. Páez AM, et al. Effects of 4-methylpyrazole on ethanol neurobehavioral toxicity in CD-1 mice. Acad Emerg Med. 2004 Aug;11(8):820-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 12.1788 mL | 60.8939 mL | 121.7878 mL | 304.4696 mL |
| 5 mM | 2.4358 mL | 12.1788 mL | 24.3576 mL | 60.8939 mL | |
| 10 mM | 1.2179 mL | 6.0894 mL | 12.1788 mL | 30.4470 mL | |
| 15 mM | 0.8119 mL | 4.0596 mL | 8.1192 mL | 20.2980 mL | |
| 20 mM | 0.6089 mL | 3.0447 mL | 6.0894 mL | 15.2235 mL | |
| 25 mM | 0.4872 mL | 2.4358 mL | 4.8715 mL | 12.1788 mL | |
| 30 mM | 0.4060 mL | 2.0298 mL | 4.0596 mL | 10.1490 mL | |
| 40 mM | 0.3045 mL | 1.5223 mL | 3.0447 mL | 7.6117 mL | |
| 50 mM | 0.2436 mL | 1.2179 mL | 2.4358 mL | 6.0894 mL | |
| 60 mM | 0.2030 mL | 1.0149 mL | 2.0298 mL | 5.0745 mL | |
| 80 mM | 0.1522 mL | 0.7612 mL | 1.5223 mL | 3.8059 mL | |
| 100 mM | 0.1218 mL | 0.6089 mL | 1.2179 mL | 3.0447 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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