2. NF-κB
    Epigenetics
    Anti-infection
  3. Keap1-Nrf2
    DNA Methyltransferase
    Virus Protease
  4. Hinokitiol

Hinokitiol  (Synonyms: β-Thujaplicin)

Cat. No.: HY-B2230 Purity: 98.97%
COA Handling Instructions

Hinokitiol is a component of essential oils isolated from Chymacyparis obtusa, reduces Nrf2 expression, and decreases DNMT1 and UHRF1 mRNA and protein expression, with anti-infective, anti-oxidative, and anti-tumor activities.

For research use only. We do not sell to patients.

Hinokitiol Chemical Structure

Hinokitiol Chemical Structure

CAS No. : 499-44-5

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10 mM * 1 mL in DMSO USD 55 In-stock
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10 mM * 1 mL
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Based on 4 publication(s) in Google Scholar

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  • Customer Review

Description

Hinokitiol is a component of essential oils isolated from Chymacyparis obtusa, reduces Nrf2 expression, and decreases DNMT1 and UHRF1 mRNA and protein expression, with anti-infective, anti-oxidative, and anti-tumor activities.

IC50 & Target[1][2]

DNMT1

 

Nrf2

 

In Vitro

In U87MG and T98G glioma cell lines, hinokitiol demonstrates a dose-dependent decrease in viability, with IC50 values of 316.5 ± 35.5 and 152.5 ± 25.3 µM, respectively. Hinokitiol represses ALDH activity and self-renewal ability in glioma stem cells, and inhibits in vitro oncogenicity. Hinokitiol also reduces Nrf2 expression in glioma stem cells in a dose-dependent manner[1]. Hinokitiol (0-100 μM) inhibits colon cancer cell growth in a dose- and time-dependent manner. Hinokitiol (5, 10 μM) decreases DNMT1 and UHRF1 mRNA and protein expression, and increases TET1 expression via enhancement of 5hmC level in HCT-116 cells. Furthermore, hinokitiol reduces methylation status and restores mRNA expression of MGMT, CHST10, and BTG4 genes[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

164.20

Appearance

Solid

Formula

C10H12O2
CAS No.
SMILES

O=C1C(O)=CC(C(C)C)=CC=C1
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (609.01 mM; Need ultrasonic)

H2O : 0.67 mg/mL (4.08 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.0901 mL 30.4507 mL 60.9013 mL
5 mM 1.2180 mL 6.0901 mL 12.1803 mL
10 mM 0.6090 mL 3.0451 mL 6.0901 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (15.23 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (15.23 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (15.23 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 98.97%

COA (248 KB) HNMR (227 KB) LCMS (255 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

U87MG and T98G glioma cells are cultured in Dulbecco's modified Eagle's medium with Ham's F12 medium (DMEM/F-12) containing 10% fetal bovine serum. Cell viability is determined using MTT to evaluate the cytotoxicity of hinokitiol. Cells are seeded in 24-well plates (1×105 cells/well) in the presence of various concentration of hinokitiol or vehicle at 37°C for 24 h followed by incubation with MTT reagent. The blue formazan crystals of viable cells are dissolved in DMSO and then evaluated spectrophotometrically at 570 nm. DMSO-treated group is set as 100%, and data are presented as percentage of DMSO control. IC50 values are calculated by the GraFit software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Hinokitiol499-44-5β-ThujaplicinKeap1-Nrf2DNA MethyltransferaseVirus ProteaseDNMTsDNA MTasesInhibitorinhibitorinhibit

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