1. NF-κB
  2. Keap1-Nrf2
    DNA Methyltransferase

Hinokitiol (Synonyms: β-Thujaplicin)

Cat. No.: HY-B2230 Purity: >98.0%
Handling Instructions

Hinokitiol is a component of essential oils isolated from Chymacyparis obtusa, with anti-infective, anti-oxidative, and anti-tumor activities.

For research use only. We do not sell to patients.
Hinokitiol Chemical Structure

Hinokitiol Chemical Structure

CAS No. : 499-44-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
50 mg USD 60 In-stock
100 mg USD 100 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Hinokitiol is a component of essential oils isolated from Chymacyparis obtusa, with anti-infective, anti-oxidative, and anti-tumor activities.

In Vitro

In U87MG and T98G glioma cell lines, hinokitiol demonstrates a dose-dependent decrease in viability, with IC50 values of 316.5 ± 35.5 and 152.5 ± 25.3 µM, respectively. Hinokitiol represses ALDH activity and self-renewal ability in glioma stem cells, and inhibits in vitro oncogenicity. Hinokitiol also reduces Nrf2 expression in glioma stem cells in a dose-dependent manner[1]. Hinokitiol (0-100 μM) inhibits colon cancer cell growth in a dose- and time-dependent manner. Hinokitiol (5, 10 μM) decreases DNMT1 and UHRF1 mRNA and protein expression, and increases TET1 expression via enhancement of 5hmC level in HCT-116 cells. Furthermore, hinokitiol reduces methylation status and restores mRNA expression of MGMT, CHST10, and BTG4 genes[2].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 6.0901 mL 30.4507 mL 60.9013 mL
5 mM 1.2180 mL 6.0901 mL 12.1803 mL
10 mM 0.6090 mL 3.0451 mL 6.0901 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

Hinokitiol is dissolved in DMSO.

U87MG and T98G glioma cells are cultured in Dulbecco's modified Eagle's medium with Ham's F12 medium (DMEM/F-12) containing 10% fetal bovine serum. Cell viability is determined using MTT to evaluate the cytotoxicity of hinokitiol. Cells are seeded in 24-well plates (1×105 cells/well) in the presence of various concentration of hinokitiol or vehicle at 37°C for 24 h followed by incubation with MTT reagent. The blue formazan crystals of viable cells are dissolved in DMSO and then evaluated spectrophotometrically at 570 nm. DMSO-treated group is set as 100%, and data are presented as percentage of DMSO control. IC50 values are calculated by the GraFit software. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight








Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 10 mM

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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