Isoimperatorin

Name: Isoimperatorin
Brand: MedChemExpress
SKU: HY-N0286
Rating: 5.0 (6 reviews)

Cat. No.: HY-N0286 Purity: 99.78%: Data Sheet
COA

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Isoimperatorin is a methanolic extract of the roots of Angelica dahurica shows significant inhibitory effects on acetylcholinesterase (AChE) with the IC₅₀ of 74.6 μM.

For research use only. We do not sell to patients.

CAS No. : 482-45-1

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Based on 6 publication(s) in Google Scholar

Other Forms of Isoimperatorin:

Isoimperatorin (Standard) In-stock
Isoimperatorin-d₆ Get quote

6 Publications Citing Use of MCE Isoimperatorin

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

Flow Cytometry

RT-PCR

: Isoimperatorin purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Aug 7;31(1):275. [Abstract]; Examination of the effect of Isoimperatorin (0.1, 0.3, 1, 3, 10, 30, 100 μM) on NCM 460 cell viability using the CCK-8 assay.

: Isoimperatorin purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Aug 7;31(1):275. [Abstract]; Cell scratch assay to examine the effect of Isoimperatorin (0.3, 1, 3 μM, 24 h) on wound healing in NCM460 cells.

: Isoimperatorin purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Aug 7;31(1):275. [Abstract]; Frequencies of Treg cells were analysed by flow cytometry treated with Isoimperatorin (0.3, 1, 3 μM).

: Isoimperatorin purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Aug 7;31(1):275. [Abstract]; The mRNA expression of Foxp3 and IL-10 was analysed by Q-PCR treated with Isoimperatorin (0.3, 1, 3 μM).

: Isoimperatorin purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Aug 7;31(1):275. [Abstract]; Immunofluorescence staining for Occludin and Muc2 in colon tissue treated with Isoimperatorin (40 mg/kg, p.o.).

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AChE BChE

Biological Activity
Purity & Documentation
References
Customer Review

Description

Isoimperatorin is a methanolic extract of the roots of Angelica dahurica shows significant inhibitory effects on acetylcholinesterase (AChE) with the IC₅₀ of 74.6 μM.

IC₅₀ & Target

AChE

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 20 μg/mL Compound: 15	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 29975532]
HepG2	IC₅₀	> 20 μg/mL Compound: 15	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 29975532]
MDA-MB-231	IC₅₀	> 20 μg/mL Compound: 15	Cytotoxicity against human MDA-MB-231 cells by MTT assay Cytotoxicity against human MDA-MB-231 cells by MTT assay	[PMID: 29975532]
Neutrophil	IC₅₀	2.6 μM Compound: 15	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochrome c based assay Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochrome c based assay	[PMID: 29975532]
Neutrophil	IC₅₀	4.2 μM Compound: 17	Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced superoxide anion generation pretreated for 5 mins by spectrophotometry Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced superoxide anion generation pretreated for 5 mins by spectrophotometry	[PMID: 24798144]
Neutrophil	IC₅₀	> 10 μM Compound: 15	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29975532]
Neutrophil	IC₅₀	> 10 μM Compound: 17	Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced elastase release for 5 mins Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced elastase release for 5 mins	[PMID: 24798144]
SH-SY5Y	EC₅₀	3.5 μM Compound: 25	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay	[PMID: 27420919]
SH-SY5Y	EC₅₀	9.2 μM Compound: 25	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 6-OHDA-induced cell death measured after 24 hrs by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 6-OHDA-induced cell death measured after 24 hrs by MTT assay	[PMID: 27420919]
SH-SY5Y	EC₅₀	> 50 μM Compound: 25	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay	[PMID: 27420919]

In Vitro

During a screening program for new agrochemicals from Chinese medicinal herbs, the ethanol extract of Notopterygium incisum rhizomes is found to possess strong nematicidal activity against the two species of nematodes, Bursaphelenchus xylophilus and Meloidogyne incognita. Based on bioactivity-guided fractionation, the four constituents are isolated from the ethanol extract and identified as Columbianetin, Falcarindiol, Falcarinol, and Isoimperatorin. Isoimperatorin also has LC₅₀ values of 21.83 μg/mL against B. xylophilus. When using 15 min UV light treatment, falcarindiol, falcarinol, and isoimperatorin demonstrated almost five times more toxic to the southern root-knot nematodes than in dark treatment while columbianetin showed only two times more toxic. isoimperatorin has been demonstrated to possess insecticidal activity against several insects, such as the cabbage aphid (Brevicoryne brassicae)^[2]. Isoimperatorin is identified in the active fraction of Angelica dahurica (AD) extract^[3]. Isoimperatorin is usually used as the internal standard (IS)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

270.28

Formula

C₁₆H₁₄O₄

CAS No.

482-45-1

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=CC2=C(OC/C=C(C)/C)C3=C(OC=C3)C=C2O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

Ethanol : 6.25 mg/mL (23.12 mM; Need ultrasonic)

DMSO : 2.5 mg/mL (9.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6999 mL	18.4993 mL	36.9987 mL
5 mM	0.7400 mL	3.6999 mL	7.3997 mL
10 mM	0.3700 mL	1.8499 mL	3.6999 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.62 mg/mL (2.29 mM); Clear solution

This protocol yields a clear solution of ≥ 0.62 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (6.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/0.1% Tween-80 in Saline water
Solubility: 6.25 mg/mL (23.12 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 50% PEG300 50% PBS
Solubility: 3.33 mg/mL (12.32 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (280 KB) SDS (394 KB)

COA (254 KB) RP-HPLC (228 KB)

Handling Instructions (2659 KB)

References

[1]. Kim DK, et al. Acetylcholinesterase inhibitors from the roots of Angelica dahurica. Arch Pharm Res. 2002 Dec;25(6):856-9. [Content Brief]

[2]. Liu G, et al. Identification of Nematicidal Constituents of Notopterygium incisum Rhizomes against Bursaphelenchus xylophilus and Meloidogyne incognita. Molecules. 2016 Sep 23;21(10). pii: E1276. [Content Brief]

[3]. Park EY, et al. Angelica dahurica Extracts Improve Glucose Tolerance through the Activation of GPR119. PLoS One. 2016 Jul 8;11(7):e0158796. [Content Brief]

[4]. Yu XA, et al. The pharmacokinetics, bioavailability and excretion of bergapten after oral and intravenous administration in rats using high performance liquid chromatography with fluorescence detection. Chem Cent J. 2016 Oct 14;10:62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	3.6999 mL	18.4993 mL	36.9987 mL	92.4967 mL
DMSO / Ethanol	5 mM	0.7400 mL	3.6999 mL	7.3997 mL	18.4993 mL
Ethanol	10 mM	0.3700 mL	1.8499 mL	3.6999 mL	9.2497 mL
	15 mM	0.2467 mL	1.2333 mL	2.4666 mL	6.1664 mL
	20 mM	0.1850 mL	0.9250 mL	1.8499 mL	4.6248 mL

Isoimperatorin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isoimperatorin482-45-1Cholinesterase (ChE)BacterialInhibitorinhibitorinhibit

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Isoimperatorin

Customer Review

Other Forms of Isoimperatorin:

Isoimperatorin Related Antibodies

6 Publications Citing Use of MCE Isoimperatorin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cholinesterase (ChE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Isoimperatorin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Isoimperatorin Related Classifications

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