1. MAPK/ERK Pathway
  2. JNK
  3. Juglanin

Juglanin 

Cat. No.: HY-N3442 Purity: >98.0%
Handling Instructions

Juglanin is a JNK activator.

For research use only. We do not sell to patients.

Juglanin Chemical Structure

Juglanin Chemical Structure

CAS No. : 5041-67-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 1093 In-stock
Estimated Time of Arrival: December 31
1 mg USD 500 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1188 In-stock
Estimated Time of Arrival: December 31
10 mg   Get quote  
50 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

View All JNK Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Juglanin is a JNK activator.

IC50 & Target[1]

JNK

 

In Vitro

Juglanin inhibits the proliferation of breast cancer cell in a dose- and time-dependent manner and presents less cytotoxic against the normal cells. Juglanin results in the accumulation of cancer cells in G2/M phase and a corresponding decrease in G0/G1 and S phases in both MCF-7 and SKBR3 cells. Juglanin up-regulates the expressions of phosphorylated Chk2, Chk2, phosphorylated Cdc25C, phosphorylated Cdc2, p27, cyclin D and down-regulates the levels of Cdc25C as well as Cdc2. The proportion of apoptosis is negligible for control cells, whereas 24 h of exposure of cells to Juglanin leads to a dose-dependent increase of apoptotic cells in both MCF-7 and SKBR3 cells. Juglanin significantly suppresses anti-apoptotic factor of Bcl-2 expression, and in contrast Bad and Bax are both up-regulated for Juglanin treatment. Juglanin markedly activates caspases and leads to Casapse-9, Casapse-8 and Caspase-3 cleavage. ROS generation is initiated by Juglanin and significantly increased by high concentrations of Juglanin. Juglanin also increases the level of JNK phosphorylation in both MCF-7 and SKBR3 cells[1].

In Vivo

Juglanin at doses of 5 and 10 mg/kg leads to significant decrease in tumor volume after 7 days of drug administration. 5 and 10 mg/kg Juglanin treatments do not cause weight loss in mice[1].

Storage

4°C, sealed storage, away from moisture

Solvent & Solubility
In Vitro: 

Methanol : 16.67 mg/mL (39.85 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3903 mL 11.9517 mL 23.9034 mL
5 mM 0.4781 mL 2.3903 mL 4.7807 mL
10 mM 0.2390 mL 1.1952 mL 2.3903 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Breast cancer cells are planted in 96-well plates with a density of 5×103 cells/well. After 12 h, the cells are treated with different concentrations of Juglanin (0 to 40 μM) for different periods of time (24 h and 48 h). Then the fresh mixture of MTS and PMS is added and incubated for 4 h at 37°C according to the manufacturer's protocol. A microplate reader is conducted to determine the absorbance at 500 nm, and the IC50 values are assessed with the probit model. Each one is performed in triplicate[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Male BALB/c-nude mice, aged 5 weeks, are used. They are maintained under specific pathogenfree conditions and supplied with sterilized food and water. On day 0, about 5×106 MCF-7 cells suspended in 0.1 mL PBS are inoculated subcutaneously in the right flank of each mouse (six mice each group). On day 9, when the tumors reach palpable size of around 200 mm3, mice are randomly assigned to three groups and receive daily intraperitoneal injection with 100 μL of vehicle (10% DMSO, 70% Cremophor/ethanol (3: 1), and 20% PBS), and 5 or 10 Juglanin mg/kg of celastrol. Tumor sizes are measured daily to observe dynamic changes in tumor growth. Body weights are also measured daily. After 7 days of drug administration, when the tumors of control group reach around 1600 mm3, all mice are killed. Tumors are dissected and stored in liquid nitrogen or fixed in formalin for further analysis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

418.35

Formula

C₂₀H₁₈O₁₀

CAS No.

5041-67-8

SMILES

O=C1C(O[[email protected]@H]2O[[email protected]@H](CO)[[email protected]](O)[[email protected]]2O)=C(C3=CC=C(O)C=C3)OC4=CC(O)=CC(O)=C41

Shipping

Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Juglanin
Cat. No.:
HY-N3442
Quantity:

Juglanin

Cat. No.: HY-N3442