Cat. No.: HY-B1000A

L-SelenoMethionine

(Synonyms: L-SeMet)

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

L-SelenoMethionine, an L-isomer of Selenomethionine, is a major natural food-form of selenium. L-SelenoMethionin is a cancer chemopreventive agent that can reduce cancer incidence by dietary supplementation and induce apoptosis of cancer cells. L-SelenoMethionine also can increase expression of glutathione peroxidase.

For research use only. We do not sell to patients.

Purity: 99.79%
CAS No.: 3211-76-5
Formula: C₅H₁₁NO₂Se
Molecular Weight:196.11

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

L-SelenoMethionine Chemical Structure

CAS No.: 3211-76-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

All Endogenous Metabolite Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	65 μM Compound: 3	Anticancer activity against human A549 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human A549 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
DU-145	IC₅₀	40 μM Compound: 3	Anticancer activity against human DU-145 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human DU-145 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
HT-29	IC₅₀	130 μM Compound: 3	Anticancer activity against human HT-29 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human HT-29 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
UACC-375	IC₅₀	50 μM Compound: 3	Anticancer activity against human UACC-375 cells assessed as cell growth inhibition measured after 6 days by SRB assay Anticancer activity against human UACC-375 cells assessed as cell growth inhibition measured after 6 days by SRB assay	[PMID: 34217061]
V79	IC₅₀	267 μM Compound: L-selenomethionine	Cytotoxicity activity against V79 cells Cytotoxicity activity against V79 cells	[PMID: 12852761]

In Vitro

L-SelenoMethionine (1-500 μM; 24-72 h) is selectively inhibits the growth of prostate cancer cells, compared with normal cells^[2].
L-SelenoMethionine (500 μM; 48 h) induce apoptosis in prostate cancer cells^[2].
L-SelenoMethionine (500 μM; 48 h) causes an increase in arrest in the G2-M phase of the cell cycle selectively in prostate cancer cells^[2].
L-SelenoMethionine (5 μM; 24 h) mitigates gene expression associated with the cellular stress response from 10 cGy irradiation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:

1, 5, 10, 50, 100, 500 μM
Incubation Time:

24, 48, 72 hours
Result:

Inhibited the growth of prostate cancer cells, with a lower IC₅₀ (1-90 μM) at 72 h than normal prostate cells (>500 μM).

Apoptosis Analysis^[2]

Cell Line:

Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:

500 μM
Incubation Time:

48 hours
Result:

Exhibited the highest level of DNA condensation in androgen-responsive LNCaP carcinoma cells, followed by PC-3 and DU145 cells.
Exhibited the nicked-end DNA labeling in prostate cancer cells.
Promoted the PARP cleavage in prostate cancer cells.

Cell Cycle Analysis^[2]

Cell Line:

Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:

500 μM
Incubation Time:

48 hours
Result:

Increased the sub-G0-G1 cell fraction of LNCaP (41.5%), PC-3 (12.1%), and DU145 cells (11.2%).
Caused a significant increases in G2 cells , particularly in LNCaP (13%), PC-3 (32%), and DU145 (20%) cells.

RT-PCR^[3]

Cell Line:

Human thyroid epithelial cells (HTori-3)
Concentration:

5 μM
Incubation Time:

24 hours
Result:

Downregulated the CDC6, GADD45A, FAS and ATF3 in irradiated cells.

In Vivo

L-SelenoMethionine (0.06-12 μg/g diet; p.o. for 3 d) partially or completely prevent the decrease in the serum or plasma levels of total antioxidants in rats exposed to gamma rays, protons or HZE particles^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 3211-76-5
Appearance Solid
Molecular Weight 196.11
Formula C₅H₁₁NO₂Se
Color White to off-white
SMILES

O=C(O)[C@@H](N)CC[Se]C
Synonyms

L-SeMet
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- other families
- Animals
- Microorganisms
- Endogenous metabolite
Widespread
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (5)

All Cell Imaging/StainingBio/Physico-chemical Assay

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Journal Impact Factor

Bioact Mater

Supramolecular self-assembly of EGCG-selenomethionine nanodrug for treating osteoarthritis. [Abstract]

2023 Oct 7:32:164-176. PMID: 37822916
Antioxidants (Basel)

L-Selenomethionine Alleviates Cryo-Induced Ferroptosis Through the NRF2-SLC7A11-GPX4 Pathway, Improving Post-Thaw In Vitro Quality of Dairy Goat Spermatozoa. [Abstract]

2026 Mar 20;15(3):392. PMID: 41897537

L-SelenoMethionine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2026 Mar 20;15(3):392. [Abstract]

Quantitative analysis showing that plasma membrane integrity was significantly higher in the 4, 6, and 8 μM L-SeMet (L-SelenoMethionine) groups than in the control (p < 0.05), with the greatest improvement observed at 6 μM.

L-SelenoMethionine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2026 Mar 20;15(3):392. [Abstract]

Quantitative analysis showing that acrosome integrity was significantly improved in the 6 μM L-SeMet (L-SelenoMethionine) group compared with the control (p < 0.05).

L-SelenoMethionine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2026 Mar 20;15(3):392. [Abstract]

Principal component analysis (PCA) showing distinct clustering between the control and L-SeMet (L-SelenoMethionine) groups, indicating clear separation of global protein expression profiles.

L-SelenoMethionine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2026 Mar 20;15(3):392. [Abstract]

Spermatozoa treated with 6 μM L-SeMet (L-SelenoMethionine) showing improved mitochondrial morphology, characterized by preserved double membranes, dense matrix, and clearly defined cristae.

L-SelenoMethionine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2026 Mar 20;15(3):392. [Abstract]

Superoxide dismutase (SOD) activity increased significantly at 4, 6, 8, and 10 μM (p < 0.05), and glutathione (GSH) concentrations were higher in all L-SeMet (L-SelenoMethionine)-treated groups (2–10 μM) than in the control (p < 0.05).
Water Biol Secur

2025 Nov 13.
J Biochem Mol Toxicol

p38MAPK/HSPB1 is involved in the regulatory effects of selenomethionine on the apoptosis, viability and testosterone secretion of sheep Leydig cells exposed to heat. [Abstract]

2024 Sep;38(9):e23826. PMID: 39188067
In Vitro Model

2025 Oct 28.

Solvent & Solubility

In Vitro:

H₂O : 14.29 mg/mL (72.87 mM; Need ultrasonic)

DMSO : 1 mg/mL (5.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.0992 mL

25.4959 mL

50.9918 mL

5 mM

1.0198 mL

5.0992 mL

10.1984 mL

10 mM

0.5099 mL

2.5496 mL

5.0992 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 9.09 mg/mL (46.35 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (789 KB)
- English - EN (789 KB)
- Français - FR (789 KB)
- Deutsch - DE (789 KB)
- Norwegian - NO (789 KB)
- Español - ES (789 KB)
- Swedish - SV (789 KB)
- Italian - IT (789 KB)
- Korean - KR (789 KB)
- Portuguese - PT (789 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Schrauzer GN, et, al. Selenomethionine: a review of its nutritional significance, metabolism and toxicity. J Nutr. 2000 Jul;130(7):1653-6. [Content Brief]

[2]. Menter DG, et, al. Selenium effects on prostate cell growth. Cancer Epidemiol Biomarkers Prev. 2000 Nov;9(11):1171-82. [Content Brief]

[3]. Jornot L, et, al. Differential regulation of glutathione peroxidase by selenomethionine and hyperoxia in endothelial cells. Biochem J. 1995 Mar 1;306 ( Pt 2)(Pt 2):581-7. [Content Brief]

[4]. Nuth M, et, al. Mitigating effects of L-selenomethionine on low-dose iron ion radiation-induced changes in gene expression associated with cellular stress. Oncol Lett. 2013 Jul;6(1):35-42. [Content Brief]

[5]. Guan J, et, al. Effects of dietary supplements on space radiation-induced oxidative stress in Sprague-Dawley rats. Radiat Res. 2004 Nov;162(5):572-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	5.0992 mL	25.4959 mL	50.9918 mL	127.4795 mL
DMSO / H₂O	5 mM	1.0198 mL	5.0992 mL	10.1984 mL	25.4959 mL
H₂O	10 mM	0.5099 mL	2.5496 mL	5.0992 mL	12.7479 mL
	15 mM	0.3399 mL	1.6997 mL	3.3995 mL	8.4986 mL
	20 mM	0.2550 mL	1.2748 mL	2.5496 mL	6.3740 mL
	25 mM	0.2040 mL	1.0198 mL	2.0397 mL	5.0992 mL
	30 mM	0.1700 mL	0.8499 mL	1.6997 mL	4.2493 mL
	40 mM	0.1275 mL	0.6374 mL	1.2748 mL	3.1870 mL
	50 mM	0.1020 mL	0.5099 mL	1.0198 mL	2.5496 mL
	60 mM	0.0850 mL	0.4249 mL	0.8499 mL	2.1247 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

L-SelenoMethionine

5 Cited Publications

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L-SelenoMethionine

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Publications Citing Use of MedChemExpress (MCE) L-SelenoMethionine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-SelenoMethionine Related Classifications

HY-B1000A Related Classifications